1887

Abstract

SUMMARY

X-ray diffraction studies of oriented gels and electron microscope studies of stained sections of the strain of tobacco rattle virus are described. The X-ray diffraction patterns are similar to those obtained by Finch (1965) and are interpreted in the same way. They show that the virus has a helical arrangement of protein subunits of pitch 25.5 ± 0.5 Å and that there is an integral number (3 + 1), in three turns of the helix. The cylindrically averaged diameter is 205 ± 5 Å. The electron miscroscope studies show that the particles pack together with a centre-to-centre distance of 203 ± 10 Å in the dried state. Two dark annular rings, of radii 41 ± 2 Å and 80 ± 4 Å, are seen in the transverse sections. The larger of these probably represents the radial position of the nucleic acid.

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/content/journal/jgv/10.1099/0022-1317-13-3-433
1971-12-01
2024-05-06
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References

  1. Bernal J. D., Fankuchen I. 1941; X-ray and crystallographic studies of plant virus preparations. III. Journal of General Physiology 25:147
    [Google Scholar]
  2. Brandes J., Paul H. L. 1957; Das Elektronenmikroskop als Hilfsmittel bei der Diagnose pflanzlicher Virosen. Betrachtungen zur Vermessung foden – under stäbchenförmigen Virusteilchen. Archiv. für Mikrobiologie 26:358
    [Google Scholar]
  3. Finch J. T. 1965; Preliminary x-ray diffraction studies of tobacco rattle and barley stripe mosaic virus. Journal of Molecular Biology 12:612
    [Google Scholar]
  4. Gregory J., Holmes K. C. 1965; Methods of preparing oriented tobacco mosaic virus sols for x-ray diffraction. Journal of Molecular Biology 13:796
    [Google Scholar]
  5. Harrison B. D., Klug A. 1966; Relation between length and sedimentation coefficient for particles of tobacco rattle virus. Virology 30:738
    [Google Scholar]
  6. Harrison B. D., Nixon H. L. 1959; Separation and properties of particles of tobacco rattle virus with different lengths. Journal of General Microbiology 21:569
    [Google Scholar]
  7. Harrison B. D., Woods R. D. 1966; Serotypes and particle dimensions of tobacco rattle viruses from Europe and America. Virology 28:610
    [Google Scholar]
  8. Labaw L. W., Rossmann M. G. 1969; Electron microscopic observations of L-lactose dehydrogenase crystals. Journal of Ultrastructural Research 27:105
    [Google Scholar]
  9. Langridge R., Wilson H. R., Hooper C. W., Wilkins M. A. F., Hamilton L. D. 1960; The molecular configuration of deoxyribose nucleic acid. I. X-ray diffraction study of a crystalline form of the lithium salt. Journal of Molecular Biology 2:19
    [Google Scholar]
  10. Lesnaw J. A., Reichmann M. E. 1970; Determination of molecular weights of plant viral protein subunits by polyacrylamide gel electrophoresis. Virology 42:724
    [Google Scholar]
  11. Lister R. M. 1966; Possible relationships of virus-specific products of tobacco rattle virus infections. Virology 28:350
    [Google Scholar]
  12. Miki T., Okada Y. 1970; Comparative studies on some strains of tobacco rattle virus. Virology 42:993
    [Google Scholar]
  13. Nixon H. L., Harrison B. D. 1959; Electron microscope evidence on the structure of the particles of tobacco rattle virus. Journal of General Microbiology 21:582
    [Google Scholar]
  14. Offord R. E. 1966; Electron microscopic observations on the substructure of tobacco rattle virus. Journal of Molecular Biology 17:370
    [Google Scholar]
  15. Offord R. E., Harris J. I. 1965; The protein subunit of tobacco rattle virus. Federation of European Biochemical Societies 2nd Meeting: Abstracts216 Vienna: Verlag der Medizinischen Akademie;
    [Google Scholar]
  16. Semancik J. S. 1970; Identity of structural protein from two isolates of TRV with different length of associated short particles. Virology 40:618
    [Google Scholar]
  17. Tollin P., Wilson H. R., Young D. W. 1968; X-ray diffraction evidence of the helical structure of narcissus mosaic virus. Journal of Molecular Biology 34:189
    [Google Scholar]
  18. Tollin P., Wilson H. R., Young D. W., Cathro A., Mowat W. P. 1967; X-ray diffraction and electron microscope studies of narcissus mosaic virus and comparison with potato virus X. Journal of Molecular Biology 26:353
    [Google Scholar]
  19. Wilson H. R., Tollin P. 1969; Some observations on the structure of potato virus X. Journal of General Virology 5:151
    [Google Scholar]
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