1887

Abstract

SUMMARY

Aerosol-inactivation of bacteriophage T is prevented by peptone and by apolar amino acids (leucine, phenylalanine). The protecting concentration is related to the salt concentration in the spray-medium, which determines the amino acid concentration in the aerosol particle after evaporation to equilibrium. The protective action of surface active agents supports the hypothesis that inactivation is due to surface inactivation. The surface occupation of the protecting amino acids was calculated with the Gibbs adsorption formula and agrees with amounts necessary to cover the air/water interface, thus preventing the phage from reaching the surface.

The decrease of amino acid concentration in the aerosol particle by surface adsorption is calculated in the appendix.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-17-1-1
1972-10-01
2024-05-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/17/1/JV0170010001.html?itemId=/content/journal/jgv/10.1099/0022-1317-17-1-1&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1948; Surface inactivation of bacterial viruses and of proteins. Journal of General Physiology 31:417–431
    [Google Scholar]
  2. Adams M. H. 1966 The Bacteriophages New York: Interscience Publishers Inc;
    [Google Scholar]
  3. Benbough J. E. 1969; The effect of relative humidity on the survival of airborne Semliki Forest virus. Journal of General Virology 4:473–477
    [Google Scholar]
  4. Benbough J. E. 1971; Some factors affecting the survival of airborne viruses. Journal of General Virology 10:209–220
    [Google Scholar]
  5. Dubovi E. J., Akers T. G. 1970; Airborne stability of tailless bacterial viruses S-13 and MS-2. Applied Microbiology 19:624–628
    [Google Scholar]
  6. Hatch M. T., Warren J. C. 1969; Enhanced recovery of airborne T3 coliphage and Pasteurella pestis bacteriophage by means of a presampling humidification technique. Applied Microbiology 17:685–689
    [Google Scholar]
  7. Hemmes J. H. 1959; De overleving van microorganismen in lucht. Academisch proefschrift (thesis) Utrecht:
    [Google Scholar]
  8. James L. K., Augenstein L. G. 1966; Adsorption of enzymes at interfaces. Advances in Enzymology 28:1–40
    [Google Scholar]
  9. de Jong J. C., Winkler K. C. 1968; The inactivation of poliovirus in aerosols. Journal of Hygiene 66:557–565
    [Google Scholar]
  10. Landolt-Bornstein 1931 Physikalisch-chemische Tabellen Berlin: Springer Verlag;
    [Google Scholar]
  11. McRitchie F., Alexander A. E. 1963; Kinetics of adsorption of proteins at interfaces. Journal of Colloid and Interface Science 18:453–463
    [Google Scholar]
  12. Mihara Y. 1966; Frost protection by fog droplets coated with monomolecular films. Nature, London 212:602–603
    [Google Scholar]
  13. Quinn P., Dawson R. M. C. 1970a; An analysis of the interaction of protein with lipid monolayers at the air/water interface. Biochemical Journal 116:671–680
    [Google Scholar]
  14. Quinn P., Dawson R. M. C. 1970b; The penetration of serum albumin into phospholipid monolayers of different fatty acid chain length and interfacial charge. Biochemical Journal 119:21–25
    [Google Scholar]
  15. Trouwborst T. 1971; Inactivering in aerosolen van microorganismen en macromoleculen. Thesis Utrecht:
    [Google Scholar]
  16. Trouwborst T., de Jong J. C., Winkler K. C. 1972; Mechanism of the inactivation of the bacteriophage T1 in aerosols. Journal of General Virology 15:235–242
    [Google Scholar]
  17. Walwick E. R., Brady J. T., Kay R. E. 1967; Evaluation of T3 coliphage injuries and efficacy of selected materials in preventing them. Applied Microbiology 15:885–892
    [Google Scholar]
  18. Warren J. C., Akers T. G., Dubovi E. J. 1969; Effect of prehumidification on sampling of selected airborne viruses. Applied Microbiology 18:893–896
    [Google Scholar]
  19. Weast R. C. 1968 Handbook of Chemistry and Physics. The Chemical Rubber Co. Ohio:, 49th edition.
    [Google Scholar]
  20. Webb S. J., Bather R., Hodges R. W. 1963; The effect of relative humidity and inositol on airborne viruses. Canadian Journal of Microbiology 9:87–92
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-17-1-1
Loading
/content/journal/jgv/10.1099/0022-1317-17-1-1
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error