1887

Abstract

SUMMARY

A standard procedure for the isolation of virus specific double-stranded RNA from cowpea mosaic virus-infected leaves is described. The double stranded RNA is characterized by its buoyant density in caesium sulphate and its melting temperature ( ). The buoyant density and the are compared with those of other double-stranded RNA’s on basis of their guanine+cytosine content. The frequency distribution of the lengths of the molecules, determined by electron microscopy, indicate the occurrence of double-stranded RNA molecules specific for each of the two RNA’s of cowpea mosaic virus. During hybridization with labelled cowpea mosaic virus RNA, the virus RNA is specifically incorporated into the double-stranded structure.

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1973-03-01
2024-05-07
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References

  1. Arnott S., Fuller W., Hodgson A., Prutton I. 1968; Molecular conformations and structure transition of RNA complementary helices and their possible biological significance. Nature, London 220:561–564
    [Google Scholar]
  2. Bishop J. M., Koch G. 1967; Purification and characterization of poliovirus induced infectious doublestranded ribonucleic acid. Journal of Biological Chemistry 29:1736–1743
    [Google Scholar]
  3. Bockstahler L. E. 1967; Biophysical studies on double-stranded RNA from turnip yellow mosaic virus-infected plants. Molecular and General Genetics 100:337–348
    [Google Scholar]
  4. Borst P., Van Bruggen E. F. J., Ruttenberg G. J. C., M. & Kroon A. M. 1969; Mitochondrial DNA II. Sedimentation analysis and electron microscopy of mitochondrial DNA from chick liver. Biochemica et biophysica acta 149:156–172
    [Google Scholar]
  5. Bove J. M. 1967; Virus de la mosa'ique jaune du navet: Synthese asymmetrique in vitro d’un segment de RNA viral. These de doctorat d’Etat es. Sci. Nat. No CNRS AO 1289 Paris:
    [Google Scholar]
  6. Burdon R. H., Billeter M. A., Weissmann C., Warner R. C., Ochoa S., Knight C. A. 1964; Replication of viral RNA V. Presence of a virus specific double-stranded RNA in leaves infected with TMV. Proceedings of the National Academy of Sciences of the United States of America 52:768–775
    [Google Scholar]
  7. Erikson R. L., Franklin R. M. 1966; Formation and properties of a replicative intermediate in the biosynthesis of viral ribonucleic acid. Bacteriological Reviews 30:267–278
    [Google Scholar]
  8. Gomatos P. J., Tamm I. 1963; The secondary structure of reovirus RNA. Proceedings of the National Academy of Sciences of the United States of America 50:878–885
    [Google Scholar]
  9. Granboulan N., Franklin R. M. 1966; Electron microscopy of viral RNA, replicative form and replicative intermediate of bacteriophage R17. Journal of Molecular Biology 22:173–177
    [Google Scholar]
  10. Kaerner H. C., Hoffmann-Berling H. 1964; Synthesis of double stranded RNA in RNA-phage infected Escherichia coli cells. Nature, London 202:1012–1013
    [Google Scholar]
  11. Kingsbury D. W. 1966; Newcastle disease virus RNA. II. Preferential synthesis of RNA complementary to parental viral RNA by chick embryo cells. Journal of Molecular Biology 18:204–214
    [Google Scholar]
  12. Miura K. I., Kimura I., Suzuki W. 1966; Double-stranded ribonucleic acid from rice dwarf virus. Virology 28:571–579
    [Google Scholar]
  13. Montagnier L., Sanders F. K. 1963; Replicative form of encephalomyocarditis virus ribunocleic acid. Nature, London 199:664–667
    [Google Scholar]
  14. Nicolaieff A., Koenig-Nikes A. M., Pinck L., Hirth L. 1970; Electron microscopy of the replicative RNA from tobacco mosaic virus and alfalfa mosaic virus: morphology and association of the doublestranded RNA. Virology 41:688–700
    [Google Scholar]
  15. Pinck L., Hirth L., Bernardi G. 1968; Isolation of replicative RNA from alfalfa mosaic virus infected plants by chromatography on hydroxyapatite columns. Biochemical and Biophysical Research Communications 31:481–487
    [Google Scholar]
  16. Solymosy F., Fedorcsak I., Gulyas A., Farkas G. L., Ehrenberg L. 1968; A new method based on the use of diethylpyrocarbonate as a nuclease inhibitor for the extraction of undegraded nucleic acid from plant tissues. European Journal of Biochemistry 5:520–527
    [Google Scholar]
  17. Steere R. L. 1956; Purification and properties of tobacco ringspot virus. Phytopathology 46:60–69
    [Google Scholar]
  18. Sueoka N., Marmur J., Doty P. 1959; Dependence of the density of deoxyribonucleic acids on guanine-cytosine content. Nature, London 183:1429–1431
    [Google Scholar]
  19. Van Griensven L. J. L., D. & Van Kammen A. 1969; The isolation of a ribonuclease-resistant RNA induced by cowpea mosaic virus: evidence for two double-stranded RNA components. Journal of General Virology 4:423–428
    [Google Scholar]
  20. Van Kammen A. 1968; The relationship between the components of cowpea mosaic virus. Two ribonucleo-protein particles necessary for the infectivity of CPMV. Virology 34:312–318
    [Google Scholar]
  21. Van Kammen A. 1971; Cowpea mosaic virus, un virus au genome divise. Physiologie vegetale 9:479–485
    [Google Scholar]
  22. Van Kammen A., Van Griensven L. J. L. D. 1970; The relationship between the components of cowpea mosaic virus. II. Further characterization of the nucleo-protein components of CPMV. Virology 41:274–280
    [Google Scholar]
  23. Weissmann C., Borst P., Burdon R. H., Billeter M. A., Ochoa S. 1964; Replication of viral RNA. III. Double-stranded replicative form of MS2 phage RNA. Proceedings of the National Academy of Sciences of the United States of America 51:682–690
    [Google Scholar]
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