1887

Abstract

SUMMARY

A murine leukaemia virus helper-independent strain of Moloney sarcoma virus (MSV), designated S + L -, was isolated from cells transformed by MSV in the absence of replicating leukaemia virus. The virus is non-infectious in tissue culture as evidenced by its failure to replicate in a variety of murine cell lines.

Sucrose gradient and gel electrophoretic analyses of the purified S + L - isolate showed that the virus contains 60 to 70 S RNA. The virus also possesses RNA-dependent DNA polymerase (RDDP) activity.

On the basis of these data it would appear that factors other than high mol. wt. RNA and RDDP may account for the lack of infectivity of this strain of MSV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-20-2-239
1973-08-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/20/2/JV0200020239.html?itemId=/content/journal/jgv/10.1099/0022-1317-20-2-239&mimeType=html&fmt=ahah

References

  1. Bassin R. H., Phillips L. A., Kramer M. J., Haapala D. K., Peebles P. T., Nomura S., Fischinger P. J. 1971a; Transformation of mouse 3T3 cells by murine sarcoma virus: release of virus-like particles in the absence of replicating murine leukemia helper virus. Proceedings of the National Academy of Sciences of the United States of America 68:1520–1524
    [Google Scholar]
  2. Bassin R. H., Phillips L. A., Kramer M. J., Haapala D. K., Peebles P. T., Nomura S., Fischinger P. J. 1971b; Properties of 3T3 cells transformed by murine sarcoma virus in the absence of replicating murine leukemia helper virus. In Proceedings Vth International Symposium of Comparative Leukemia Research, Padova, Italy Edited by Chieco-Banchi. L., Dutcher. R. M.
    [Google Scholar]
  3. Bassin R. H., Simons P. J., Chesterman F. C., Harvey J. J. 1968; Murine sarcoma virus (Harvey): Characteristics of focus formation in mouse embryo cell cultures, and virus production by hamster tumor cells. International Journal of Cancer 3:265–272
    [Google Scholar]
  4. Bassin R. H., Tuttle N., Fischinger P. J. 1970; Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures. International Journal of Cancer 6:95–107
    [Google Scholar]
  5. Fischinger P. J., Schafer W., Seifert E. 1972; Detection of some murine leukemia virus antigens in virus particles derived from 3T3 cells transformed only by murine sarcoma virus. Virology 47:229–235
    [Google Scholar]
  6. Gazdar A. F., Phillips L. A., Sarma P. S., Peebles P. T., Chopra H. C. 1971; Presence of sarcoma genome in a ‘non-infectious’ mammalian virus. Nature New Biology 234:69–72
    [Google Scholar]
  7. Hartley J. W., Rowe W. P. 1966; Production of altered cell foci in tissue culture by defective Moloney sarcoma virus particles. Proceedings of the National Academy of Sciences of the United States of America 55:780–786
    [Google Scholar]
  8. Naso R. B., Vidrine J. G., Wang C. S., Arlinghaus R. B. 1972; C-type virus particles produced by normal mouse spleen-thymus cultures. Virology 47:521–524
    [Google Scholar]
  9. O’conner T. E., Fischinger P. J. 1968; Titration patterns of a murine sarcoma-leukemia virus complex: Evidence for existence of competent sarcoma virions. Science, New York 159:325–329
    [Google Scholar]
  10. Vidrine J. G., Wang C. S., Arlinghaus R. B. 1972; Stimulation of amino acid incorporation by Rauscher murine leukemia virus RNA in a cell-free system. Biochemical and Biophysical Research Communications 46:538–544
    [Google Scholar]
  11. Wright B. S., Lasfarques J. C. 1965; Long-term propagation of the Rauscher murine leukemia virus in tissue culture. Journal of the National Cancer Institute 35:319–327
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-20-2-239
Loading
/content/journal/jgv/10.1099/0022-1317-20-2-239
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error