1887

Abstract

SUMMARY

The plaquing characteristics of a comprehensive range of influenza viruses in epithelial cell cultures of bovine and specific-pathogen-free chicken kidney are described. All type B viruses produced standard large plaques with high efficiency in bovine kidney cell cultures. They also plaqued in chicken kidney, but morphology was more varied and efficiency was slightly reduced. By comparison, the behaviour of a range of influenza A viruses was heterogeneous. Five out of 16 viruses failed to plaque in calf kidney cell cultures and the plaque morphology of the remainder varied with individual isolates and not with serotypes. E.o.p. was variable. Results in chicken kidney cell cultures were better and there were relatively fewer non-plaque-formers. Influenza A recombinants generally behaved like other influenza A viruses and it was concluded that plaquing capacity was genetically determined.

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/content/journal/jgv/10.1099/0022-1317-22-3-347
1974-03-01
2024-05-06
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References

  1. Beare A. S., Hall T. S. 1971; Recombinant influenza A viruses as live vaccines for man. Lancet ii:1271–1273
    [Google Scholar]
  2. Beare A. S., Keast K. 1971; Plaque formation by influenza B viruses in primary calf kidney cell monolayers. Journal of General Virology 13:321–325
    [Google Scholar]
  3. Kilbourne E. D. 1968; Recombination of influenza A viruses of human and animal origin. Science, New York 160:74–76
    [Google Scholar]
  4. Kilbourne E. D. 1969; Future influenza vaccines and the use of genetic recombinants. Bulletin of the World Health Organization 41:643–645
    [Google Scholar]
  5. Leibovitz A. 1963; The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. American Journal of Hygiene 78:173–180
    [Google Scholar]
  6. Maassab H. F. 1959; The propagation of multiple viruses in chick kidney cultures. Proceedings of the National Academy of Sciences of the United States of America 45:1035–1039
    [Google Scholar]
  7. Maassab H. F. 1968; Plaque formation of influenza virus at 25 °C. Nature, London 219:645–646
    [Google Scholar]
  8. McCahon D., Schild G. C. 1972; Segregation of antigenic and biological characteristics during influenza virus recombination. Journal of General Virology 15:73–77
    [Google Scholar]
  9. Murphy B. R., Chalhub E. G., Musinoff S. R., Chanock R. M. 1972; Temperature-sensitive mutants of influenza virus. II. Attenuation of ts recombinants for man. Journal of Infectious Diseases 126:170–178
    [Google Scholar]
  10. Okuno Y., Nakamura K. 1966; Prophylactic effectiveness of live influenza vaccine in 1965. Bikerfs Journal 9:89–95
    [Google Scholar]
  11. World Health Organization 1971; A revised system of nomenclature for influenza viruses. Bulletin of the World Health Organization 45:119–124
    [Google Scholar]
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