1887

Abstract

SUMMARY

Comparative karyological studies have been made on the J-96 line of human leukaemic cells, which are susceptible to enteroviruses, and on cell strains derived from this line which are persistently infected with Coxsackie B5 virus or free from infective virus but possessing high specific resistance to Coxsackie B3 or B5 viruses. It was shown that the karyotypes of these cell strains were characterized by reduced numbers of small acrocentric chromosomes of group G. It is suggested that group G chromosomes in cells of human origin incorporate genes which control alkaline phosphatase activity and the production of specific substances essential to adsorption and intracellular development of Coxsackie B viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-23-1-17
1974-04-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/23/1/JV0230010017.html?itemId=/content/journal/jgv/10.1099/0022-1317-23-1-17&mimeType=html&fmt=ahah

References

  1. Alter A. A., Lee S. L., Pourfar M., Dobkin G. 1963; Studies of leucocyte alkaline phosphatase in mongolism: a possible chromosome marker. Blood 22:165–177
    [Google Scholar]
  2. Amchenkova A. M., Gulevitch N. E. 1968; Morphological and cytochemical study of leukaemic cells sensitive and resistant to Coxsackie B3 virus. Voprosȳ virusologii 13 no. 1 67–72
    [Google Scholar]
  3. Amchenkova A. M., Sovjetova G. P. 1968; Cytologic mechanisms of specific anti-viral immunity. Voprosȳ virusologii 13 no. 5 560–566
    [Google Scholar]
  4. Chessin L. N., Hirschhorn K. 1961; Virus resistance and sensitivity in cultured human synovial cells as a possible genetic marker. Experimental Cell Research 23:138–144
    [Google Scholar]
  5. De Carli L., Maio J. J., Nuzzo F. 1963; Alkaline phosphatase activity and chromosome variation in human cells in culture. Journal of the National Cancer Institute 31:1501–1509
    [Google Scholar]
  6. Ebina T., Takahashi K., Homma M., Ishida N. 1969; Alkaline phosphatase activity of HeLa S3 cells persistently infected with hemadsorption type 2 virus. Virology 39:597–599
    [Google Scholar]
  7. Gulevitch N. E., Bahutashvily V. L., Grinberg K. N. 1970; Comparative study of chromosomes in virus-sensitive and -resistant cells. In Materials of the XVth All-Union Conference of epidemiologists, microbiologists and infectionists, Moscow 2:276–277
    [Google Scholar]
  8. Khesin YA. E., Amchenkova A. M. 1972; On the relationship between the enzymatic composition and the susceptibility of the cell to viruses. Journal of General Virology 15:105–111
    [Google Scholar]
  9. Königsberg U. R., Nitowsky H. M. 1964; Studies of the karyotype of clonal strains of Chang liver differing in alkaline phosphatase activity. Journal of the National Cancer Institute 29:699–710
    [Google Scholar]
  10. Kusano T., Wang R., Pollack R., Green H. 1970; Human-mouse hybrid lines and susceptibility to poliovirus. II. Polio sensitivity and chromosome constitution of the hybrids. Journal of Virology 5:682–685
    [Google Scholar]
  11. Moorhead P. S., Nowell P. C., Mellmann W. J., Battips D. M., Hugeford D. A. 1960; Chromosome preparations of leucocyte cultures from human peripheral blood. Experimental Cell Research 20:613–616
    [Google Scholar]
  12. Osgood E. E., Brooke J. H. 1955; Continuous tissue culture of leucocytes from human leucemic bloods by application of ‘gradient’ principles. Blood 10:1010–1022
    [Google Scholar]
  13. Patau K. 1960; The identification of individual chromosomes, especially in man. American Journal of Human Genetics 12:250–254
    [Google Scholar]
  14. Robinson A. 1960; A proposed standard system of nomenclature of human mitotic chromosomes. Journal of the American Medical Association 174:159–162
    [Google Scholar]
  15. Soloviev V. D., Khesin Y. A. E. 1970; Chronic infection and antiviral immunity of cells. Vestnik Academii meditsinskikh nauk SSSR no. 10 20–31
    [Google Scholar]
  16. Sovjetova G. P., Marchenko V. I., Amchenkova A. M., Balandin I. G., Lushnikov A. A. 1971; Persistent virus infection in continuous cultures of human leukemia cells (J-96) and murine fibroblasts (L) produced by inoculation of cultures with Coxsackie B5 and vesicular stomatitis viruses in the presence of antisera to the infected cells. Voprosȳ virusologii 16 no. 1 10–16
    [Google Scholar]
  17. Varshaver N. B., Gulevitch N. E. 1964; Studies in genetic mechanism of cell immunity. 2. A karyological investigation of resistant leukemia cell lines. Voprosȳ virusologii 9 no. 4 482–489
    [Google Scholar]
  18. Vogt M. 1959; A study of the relationship between karyotype and phenotype in cloned lines of strain HeLa. Genetics 44:1257–1270
    [Google Scholar]
  19. Walker D. L. 1964; The viral carrier state in animal cell culture. In Progress in Medical Virology 6:111–148
    [Google Scholar]
  20. Wang R., Pollack R., Kusano T., Green H. 1970; Human-mouse hybrid cell lines and susceptibility to poliovirus. 1. Conversion from polio sensitivity to polio resistance accompanying loss of human gene-dependent polio receptors. Journal of Virology 5:677–681
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-23-1-17
Loading
/content/journal/jgv/10.1099/0022-1317-23-1-17
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error