1887

Abstract

SUMMARY

Antibodies induced by the injection of alfalfa mosaic virus (AMV) or the protein of turnip crinkle virus (TCV) reacted with the viruses and their respective protein subunits in gel-diffusion tests. The ratios of virus-reactive antibodies to protein-reactive antibodies were different in two fractions obtained by chromatography of the sera on DEAE-Sephadex. The first DEAE-Sephadex fraction contained a greater proportion of virus-reactive antibodies than did the second fraction or the unfractionated antiserum. Electrophoretic mobility measurements at pH 8.6 showed that AMV and TCV had a greater negative charge per unit surface area than did their respective proteins.

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1974-08-01
2024-05-05
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References

  1. Allen W. R., Tremaine J. H. 1965; Characteristics of antigens and antibodies associated with the Prunus necrotic ringspot virus immune system. Virology 25:122–132
    [Google Scholar]
  2. Ansevin A. T., Stevens C. L., Lauffer M. A. 1964; Polymerization-depolymerization of tobacco mosaic virus protein. III. Changes in ionization and in electrophoretic mobility. Biochemistry 3:1512–1518
    [Google Scholar]
  3. Brown F. 1958; Immunoelectrophoretic evidence for the presence of two precipitating antibodies in serum following infection with the virus of foot-and-mouth disease. Nature, London 181:1130–1131
    [Google Scholar]
  4. Hamilton R. I. 1961; Properties of brome mosaic virus and its related antigens. Virology 15:452–464
    [Google Scholar]
  5. Kelly J. J., Kaesberg P. 1962; Preparation of protein subunits from alfalfa mosaic virus under mild conditions. Biochimica et biophysica acta 55:236–237
    [Google Scholar]
  6. Leberman R. 1968; The disaggregation and assembly of simple viruses. In The Molecular Biology of Viruses pp 183–206 Edited by Crawford L. V., Stoker M. G. P. Cambridge: University Printing House;
    [Google Scholar]
  7. Moed J. R., Veldstra H. 1968; Alfalfa mosaic virus: comparative investigation of top component and bottom component by means of finger-printing and immunological techniques. Virology 37:404–415
    [Google Scholar]
  8. Mozes E., Robbins J. B., Sela M. 1967; Heterogeneity of the light chains of rabbit immunoglobulin G fractions and of a series of antibodies directed towards antigens of differing complexity. Immuno-chemistry 4:239–245
    [Google Scholar]
  9. Rude E., Mozes E., Sela M. 1968; Role of the net electrical charge of the complete antigen in determining the chemical nature of anti-p-azobenzenearsonate antibodies. Biochemistry 7:2971–2975
    [Google Scholar]
  10. Scheele R. B., Lauffer M. A. 1967; Acid-base titrations of tobacco mosaic virus and tobacco mosaic virus protein. Biochemistry 6:3076–3081
    [Google Scholar]
  11. Sela M., Givol D., Mozes E. 1963; Resolution of rabbit y-globulin into two fractions by chromatography on diethyl-aminoethyl-Sephadex. Biochimica et biophysica acta 78:649–657
    [Google Scholar]
  12. Sela M., Mozes E. 1966; Dependence of the chemical nature of antibodies on the net electrical charge of antigens. Proceedings of the National Academy of Sciences of the United States of America 55:445–452
    [Google Scholar]
  13. Tremaine J. H., Stace-Smith R. 1969; Amino acid analyses of two strains of alfalfa mosaic virus. Phytopathology 59:521–522
    [Google Scholar]
  14. Van regenmortel M. H. V. 1966; Plant virus serology. Advances in Virus Research 12:207–271
    [Google Scholar]
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