1887

Abstract

SUMMARY

Mouse L cell interferon that had been either partially or completely inactivated by heating at 56 °C, boiling at 100 °C, shaking, repeated freezing and thawing, or treatment with mercaptoethanol and urea could be partly reactivated by addition of the anionic detergents, decyl-, dodecyl-, or tetradecyl sulphate. For complete reactivation it was necessary to disrupt disulphide bonds of interferon in solutions containing detergents.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-24-3-567
1974-09-01
2024-05-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/24/3/JV0240030567.html?itemId=/content/journal/jgv/10.1099/0022-1317-24-3-567&mimeType=html&fmt=ahah

References

  1. Edy V., Billiau A., De Somer P. 1974; Stabilization of mouse and human interferons by acid pH against inactivation due to shaking and guanidine hydrochloride. Proceedings of the Society for Experimental Biology and Medicine 146:249–253
    [Google Scholar]
  2. Fish W. W., Reynolds J. A., Tanford C. 1970; Gel chromatography of proteins in denaturing solvents. Journal of Biological Chemistry 245:5166–5168
    [Google Scholar]
  3. Ke H. Y., Ho M. 1968; Studies on physicochemical inactivation of rabbit interferons. Proceedings of the Society for Experimental Biology and Medicine 129:433–436
    [Google Scholar]
  4. Mogensen K. E., Cantell K. 1974; Human leukocyte interferon: role for disulphide bond. Journal of General Virology 22:95–103
    [Google Scholar]
  5. Ng M. H., Vilcek J. 1972; Interferons: physicochemical properties and control of cellular synthesis. Advances in Protein Chemistry 26:173–241
    [Google Scholar]
  6. Stewart W. E. 1974; Distinct molecular species of interferons. Virology (in the press)
    [Google Scholar]
  7. Stewart W. E., DE Clercq E., DE somer P. 1974; Stabilization of interferons by ‘defensive’ reversible denaturation. Nature, London 249:460–461
    [Google Scholar]
  8. Weil R., Dorner F. 1973; Interferon structure: facts and speculations. In Selective Inhibitors of Viral Functions107–122 Edited by Carter W. A. Cleveland: CRC Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-24-3-567
Loading
/content/journal/jgv/10.1099/0022-1317-24-3-567
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error