f Sequential Steps in Attachment of Human Rhinovirus Type 2 to HeLa Cells
- Authors: Jane Noble-Harvey, K. Lonberg-Holm
- First Published Online: 01 October 1974, Journal of General Virology 25: 83-91, doi: 10.1099/0022-1317-25-1-83
- Subject: Articles
- Issue Published:
When human rhinovirus type 2 attaches to HeLa cells, it first forms a reversible complex which can be dissociated by addition of EDTA to the medium. After prolonged incubation at 25 °C, most of the cell-associated virus progresses to an irreversible complex from which it cannot be eluted by EDTA, although some noninfectious material elutes spontaneously. The addition of a sufficient concentration of SDS to block cell-mediated eclipse of the virus does not block the formation of the irreversible complex at 25 °C, while virus adsorbed and incubated at 0 °C remains reversibly bound. The number of receptor sites per cell is not influenced by SDS, but at 0 °C cells appear to have fewer receptor sites than at physiological temperature.
© Society for General Microbiology, 1974 | Published by the Microbiology Society
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