1887

Abstract

SUMMARY

Using a non-cytotoxic dose of ethidium bromide, a differential inhibitor of mitochondrial DNA and RNA synthesis, some early event(s), required after infection of Balb 3T3 cells with murine sarcoma virus, did not occur. This led to an inhibition of virus replication. The cells became refractory to inhibition 24 h after infection. The most effective inhibition was observed when cells were treated with ethidium bromide for 15 to 18 h before infection, suggesting the depletion of a cellular material involved in the establishment of virus infection. The degree of inhibition by ethidium bromide appeared to be affected by the multiplicity of infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-25-2-197
1974-11-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/25/2/JV0250020197.html?itemId=/content/journal/jgv/10.1099/0022-1317-25-2-197&mimeType=html&fmt=ahah

References

  1. Bader A. V. 1973; Role of mitochondria in the production of RNA-containing tumor viruses. Journal of Virology 11:314–324
    [Google Scholar]
  2. Balcavage W. X., Baxter-Gabbard K. L., Ko M., Rea M., Padgett F., Levine A. S. 1972; Mitochondrial alterations associated with avian reticuloendotheliosis virus (strain T) pathogenicity. Biochemical and Biophysical Research Communications 48:605–611
    [Google Scholar]
  3. Bishop J. M. 1974; Viral Transformation and Endogenous Viruses. Flexner Symposium Vanderbilt University; Nashville, Tenn., U.S.A:
    [Google Scholar]
  4. Bose S. K., Zlotnick B. J. 1973a; Growth- and density-dependent inhibition of deoxyglucose transport in Balb 3T3 cells and its absence in cells transformed by murine sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 70:2374–2378
    [Google Scholar]
  5. Bosmann H. B. 1971; Mitochondrial biochemical events in a synchronized mammalian cell population. Journal of Biological Chemistry 246:3817–3823
    [Google Scholar]
  6. Clayton D. A., Vinograd J. 1969; Complex mitochondrial DNA in leukemic and normal human myeloid cells. Proceedings of the National Academy of Sciences of the United States of America 62:1124–1129
    [Google Scholar]
  7. Gadaleta M. N., Greco M., Saccone C. 1970; The effect of rifampicin on mitochondrial RNA polymerase from rat liver. FEES Letters 10:54–56
    [Google Scholar]
  8. Gelb L. D., Aaronson S. A., Martin M. A. 1971; Heterogeneity of murine leukemia virus in vitro DNA;detection of viral DNA in mammalian cells. Science, New York 172:1353–1355
    [Google Scholar]
  9. Hirschman S. Z. 1971; Inhibitors of DNA polymerases of murine leukemia viruses: Activity of ethidium bromide. Science, New York 173:441–443
    [Google Scholar]
  10. Jackisch R., Jung A., Schlegel W., Mayer D. 1972; Nachweis und anreicherung einer rifampicin-insensitiven DNA-abhangigen RNA-polymerase aus Ehrlich-ascites-tumorzell-mitochondrien. Hoppe-Seyler’s Zeitschrift fur Physiologische Chemie 353:1705–1715
    [Google Scholar]
  11. Kara J., Mach O., Cerna H. 1971; Replication of Rous sarcoma virus and biosynthesis of the oncogenic subviral ribonucleoprotein particles (‘virosomes’) in the mitochondria isolated from Rous sarcoma tissue. Biochemical and Biophysical Research Communications 44:162–170
    [Google Scholar]
  12. King M. E., Godman G. C., King D. W. 1972; Respiratory enzymes and mitochondrial morphology of HeLa and L cells treated with chloramphenicol and ethidium bromide. Journal of Cell Biology 53:127–142
    [Google Scholar]
  13. Mukerjee H., Goldfeder A. 1973; Purification and properties of ribonucleic acid polymerase from rat liver mitochondria. Biochemistry 12:5096–5101
    [Google Scholar]
  14. Nass M. M. K. 1970; Abnormal DNA patterns in animal mitochondria: Ethidium bromide-induced breakdown of closed circular DNA and conditions leading to oligomer accumulation. Proceedings of the National Academy of Sciences of the United States of America 67:1926–1933
    [Google Scholar]
  15. Nass M. M. K. 1972; Differential effects of ethidium bromide on mitochondrial and nuclear DNA synthesis in vivo in cultured mammalian cells. Experimental Cell Research 72:211–222
    [Google Scholar]
  16. Nass M. M. K. 1973; Temperature-dependent formation of dimers and oligomers of mitochondrial DNA in cells transformed by a thermosensitive mutant of Rous sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 70:3739–3743
    [Google Scholar]
  17. Richert N. J., Hare J. D. 1972; Distinctive effects of inhibitors of mitochondrial function on Rous sarcoma virus replication and malignant transformation. Biochemical and Biophysical Research Communications 46:5–10
    [Google Scholar]
  18. Robinson W. S., Pitkanen A., Rubin H. 1965; The nucleic acid of the Bryan strain of Rous sarcoma virus: purification of the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences of the United States of America 54:137–144
    [Google Scholar]
  19. Robinson H. L., Robinson W. S. 1971; Inhibition of growth of uninfected and Rous sarcoma virus-infected chick-embryo fibroblasts by rifampicin. Journal of the National Cancer Institute 46:785–788
    [Google Scholar]
  20. Salzberg S., Robin M. S., Green M. 1973; Appearance of virus-specific RNA, virus particles, and cell surface changes in cells rapidly transformed by the murine sarcoma virus. Virology 53:186–195
    [Google Scholar]
  21. Schmerling Z. G. 1969; The effect of rifamycin on RNA synthesis in the rat liver mitochondria. Biochemical and Biophysical Research Communications 37:965–969
    [Google Scholar]
  22. Shoyab M., Baluda M. A., Evans R. 1974; Acquisition of new DNA sequences after infection of chicken cells with avian myeloblastosis virus. Journal of Virology 13:331–339
    [Google Scholar]
  23. Soslau G., Nass M. M. K. 1971; Effects of ethidium bromide on the cytochrome content and ultrastructure of L cell mitochondria. Journal of Cell Biology 51:514–524
    [Google Scholar]
  24. Ting R. C., Yang S. S., Gallo R. C. 1972; Reverse transcriptase, RNA tumor virus transformation and derivatives of rifamycin SV. Nature New Biology 236:163–166
    [Google Scholar]
  25. Waring M. J. 1965; Complex formation between ethidium bromide and nucleic acids. Journal of Molecular Biology 13:269–282
    [Google Scholar]
  26. Zlotnick B. J., Bose S. K., Roa R. C. 1974; Deoxyglucose transport changes in murine sarcoma virus-infected cells – a quantitative assay for virus transforming activity. Journal of General Virology 24:365
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-25-2-197
Loading
/content/journal/jgv/10.1099/0022-1317-25-2-197
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error