1887

Abstract

SUMMARY

Light and electron microscopy of spinach and leaf tissue infected with parsnip yellow fleck virus (PYFV) and of chervil containing PYFV either alone or together with its ‘helper’ virus, anthriscus yellows (AYV), showed that cells of all species contained inclusion bodies not found in virus-free plants. Inclusions occurred in most types of leaf cell and consisted of vesicles probably derived from the endoplasmic reticulum, other smaller vesicular structures, and straight tubules about 30 nm in diam.; mitochondria and Golgi bodies often occurred around the periphery. Older inclusion bodies were composed almost entirely of the straight tubules.

Tubules about 45 nm in diam. and containing virus-like particles were often associated with the plasmodesmata and were sheathed by outgrowths of cell-wall material. Tubular structures occurred also in the sieve tubes and some of them contained virus-like particles. Most cell organelles appeared normal but many chloroplasts possessed peripheral vesicles bounded by a single membrane and some contained rectangular microcrystalline structures. No effects attributable to AYV were noted.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-26-3-277
1975-03-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/26/3/JV0260030277.html?itemId=/content/journal/jgv/10.1099/0022-1317-26-3-277&mimeType=html&fmt=ahah

References

  1. Carroll T. W. 1970; Relation of barley stripe mosaic virus to plastids. Virology 42:1015–1022
    [Google Scholar]
  2. Conti G. G., Vegetti G., Bassi M., Fa Vali M. A. 1972; Some ultrastructural and cytochemical observations on Chinese cabbage leaves infected with cauliflower mosaic virus. Virology 47:694–700
    [Google Scholar]
  3. De Zoeten G. A., Assink A. M., Van Kammen A. 1974; Association of cowpea mosaic virus-induced doublestranded RNA with a cytopathological structure in infected cells. Virology 59:341–355
    [Google Scholar]
  4. El Nagar S., Murant A. F. 1973 Parsnip yellow fleck virus. Report of the Scottish Horticultural Research Institute for 1972 p. 66
    [Google Scholar]
  5. Esau K. 1968 In Viruses in Plant Hosts pp. 7–20 Madison: University of Wisconsin Press;
    [Google Scholar]
  6. Gerola F. M., Bassi M. 1966; An electron microscopy study of leaf vein tumours from maize plants experimentally infected with maize rough dwarf virus. Caryologia 19:13–40
    [Google Scholar]
  7. Halk E. L., Mcguire J. M. 1973; Translocation of tobacco ringspot virus in soybean. Phytopathology 63:1291–1300
    [Google Scholar]
  8. Hatta T., Bullivant S., Matthews R. E. F. 1973; Fine structure of vesicles induced in chloroplasts of Chinese cabbage leaves by infection with turnip yellow mosaic virus. Journal of General Virology 20:37–50
    [Google Scholar]
  9. Hooper G. R., Wiese M. V. 1972; Cytoplasmic inclusions in wheat affected by wheat spindle streak mosaic. Virology 47:664–672
    [Google Scholar]
  10. Jones A. T., Kinninmonth A. M., Roberts I. M. 1973; Ultrastructural changes in differentiated leaf cells infected with cherry leaf roll virus. Journal of General Virology 18:61–64
    [Google Scholar]
  11. Kim K. S., Fulton J. P. 1971; Tubules with virus-like particles in leaf cells infected with bean pod mottle virus. Virology 43:329–337
    [Google Scholar]
  12. Kitajima E. W., Lauritis J. A. 1969; Plant virions in plasmodesmata. Virology 37:681–684
    [Google Scholar]
  13. Kolehmainen L., Zech H., Von Wettstein D. 1965; The structure of cells during tobacco mosaic virus reproduction. Journal of Cell Biology 35:77–97
    [Google Scholar]
  14. Matthews R. E. F. 1973; Induction of disease by viruses, with special reference to turnip yellow mosaic virus. Annual Review of Phytopathology 11:147–170
    [Google Scholar]
  15. Murant A. F., Goold R. A. 1968; Purification, properties and transmission of parsnip yellow fleck virus, a semi-persistent aphid-borne virus. Annals of Applied Biology 62:123–137
    [Google Scholar]
  16. Murant A. F., Roberts I. M., Goold R. A. 1973; Cytopathological changes and extractable infectivity in Nicotiana clerelandii leaves infected with carrot mottle virus. Journal of General Virology 21:269–283
    [Google Scholar]
  17. Peterson J. F. 1970; Electron microscopy of soil-borne wheat mosaic virus in host cells. Virology 42:304–310
    [Google Scholar]
  18. Roberts I. M., Harrison B. D. 1970; Inclusion bodies and tubular structures in Chenopodium amaranticolor plants infected with strawberry latent ringspot virus. Journal of General Virology 7:47–54
    [Google Scholar]
  19. Roberts I. M., Hutcheson A. M. 1974 Handling and staining epoxy resin sections for light microscopy. Journal of Microscopy (in the press)
    [Google Scholar]
  20. Robertson W. M., Roberts I. M. 1972; A simple device for the hulk staining and storage of ultrathin sections on grids. Journal of Microscopy 95:425–428
    [Google Scholar]
  21. Van Der Scheer C., Groenewegen J. 1971; Structure in cells of Vigna unguiculada infected with cowpea mosaic virus. Virology 46:493–497
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-26-3-277
Loading
/content/journal/jgv/10.1099/0022-1317-26-3-277
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error