1887

Abstract

SUMMARY

Brome mosaic virus (BMV) can be radioactively labelled by allowing virus synthesis to take place in barley leaves whose cut ends are placed in solutions of radioactive precursors of BMV. When barley plants are infected by inoculation of the first emerging leaf, the rate of incorporation of both [C]-leucine and [P]-orthophosphate into virus particles was found to be maximal in the second leaf, cut and labelled 4 to 5 days after inoculation. The rate of incorporation declined when the labelling or the cutting and labelling were delayed, even though assembly of particles still took place from pre-formed components. An exposure of 72 h to an appropriate amount of P yielded highly radioactive BMV RNA for possible sequencing studies. A specific radioactivity (P) of the order of 1 mCi/mg RNA can be obtained readily.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-26-3-321
1975-03-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/26/3/JV0260030321.html?itemId=/content/journal/jgv/10.1099/0022-1317-26-3-321&mimeType=html&fmt=ahah

References

  1. Argetsinger Steitz J. 1969; Polypeptide chain initiation: Nucleotide sequence of the three ribosomal binding sites in bacteriophage R17 RNA. Nature, London 224:957–967
    [Google Scholar]
  2. Bockstahler L. E., Kaesberg P. 1965; Infectivity studies of brome grass mosaic virus RNA. Virology 27:418–424
    [Google Scholar]
  3. Dahlberg J. E. 1968; Terminal sequences of bacteriophage RNAs. Nature, London 220:548–552
    [Google Scholar]
  4. Hadidi A., Fraenkel-Conrat H. 1973; Characterization and specificity of soluble RNA polymerase of brome mosaic virus. Virology 52:363–372
    [Google Scholar]
  5. Horst J., Fraenkel-Conrat H., Mandeles S. 1971; Terminal heterogeneity of both ends of the satellite tobacco necrosis virus ribonucleic acid. Biochemistry 10:4748–4752
    [Google Scholar]
  6. Matthews R. E. F. 1970 In Plant Virology New York and London: Academic Press;
    [Google Scholar]
  7. Semal J., Kummert J. 1971; Sequential synthesis of double-stranded and single-stranded RNA by cell-free extracts of barley leaves infected with brome mosaic virus. Journal of General Virology 10:79–89
    [Google Scholar]
  8. Shih D. S., Lane L. C., Kaesberg P. 1972; Origin of the small component of brome mosaic virus. Journal of Molecular Biology 64:353–362
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-26-3-321
Loading
/content/journal/jgv/10.1099/0022-1317-26-3-321
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error