1887

Abstract

SUMMARY

RNA extracted from cells previously stimulated to synthesize the antiviral protein, interferon, causes species-specific interferon synthesis when added to heterospecific cell cultures. Our results confirm the report of De Maeyer-Guignard, De Maeyer & Montagnier (1972). We have used this observation to obtain a relative quantitative assay for the interferon messenger RNA activity. At appropriate concentrations, the yield of interferon is proportional to the concentration of RNA adsorbed to recipient cell cultures.

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1975-05-01
2024-04-28
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References

  1. Aviv H., Packman S., Swan D., Ross J., Leder P. 1973; In vitro synthesis of DNA complementary to mRNA derived from a light chain-producing myeloma tumor. Nature New Biology 241:174–176
    [Google Scholar]
  2. Berns A. J. M., Van kraaikamp M., Bloemendal H., Lane C. D. 1972; Calf crystallin synthesis in frog cells: the translation of lens-cell 14S RNA in oocytes. Proceedings of the National Academy of Sciences of the United States of America 69:1606–1609
    [Google Scholar]
  3. Bhoopalam N., Yakulis V., Costea N., Heller P. 1972; Surface immunoglobulins of circulating lymphocytes in mouse plasmacytoma. II. The influence of plasmacytoma RNA on surface immunoglobulins of lymphocytes. Blood 39:465–471
    [Google Scholar]
  4. Brachet J., Huez G., Hubert E. 1973; Microinjection of rabbit hemoglobin messenger RNA into amphibian oocytes and embryos. Proceedings of the National Academy of Sciences of the United States of America 70:543–547
    [Google Scholar]
  5. De maeyer-guignard J., De maeyer E., Montagnier L. 1972; Interferon messenger RNA: translation in heterologous cells. Proceedings of the National Academy of Sciences of the United States of America 69:1203–1207
    [Google Scholar]
  6. Diggelman H., Faust c. H., Mach B. 1973; Enzymatic synthesis of DNA complementary to purified 14S messenger RNA of immunoglobulin light chain. Proceedings of the National Academy of Sciences of the United States of America 70:693–696
    [Google Scholar]
  7. Duesberg P. H., Robinson W. S. 1965; Isolation of the nucleic acid of Newcastle disease virus. Proceedings of the National Academy of Sciences of the United States of America 54:794–800
    [Google Scholar]
  8. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with poliomyelitis virus. Journal of Experimental Medicine 99:167–182
    [Google Scholar]
  9. Finter N. B. 1973; Interferon assay methods. In Interferons and Their Inducers pp. 139–142 Edited by Finter N. B. New York: American Elsevier;
    [Google Scholar]
  10. Gross K., Ruderman J., Jacobs-lorena M., Baglioni C., Gross P. R. 1973; Cell-free synthesis of histones directed by messenger RNA from sea urchin embryos. Nature New Biology 241:272–274
    [Google Scholar]
  11. Gurdon J. B., Lane C. D., Woodland H. R., Marbaix G. 1971; The use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells. Nature, London 233:177–182
    [Google Scholar]
  12. Harris S. E., Means A. R., Mitchell W. M., O’malley B. W. 1973; Synthesis of [3H]DNA complementary to ovalbumin messenger RNA: evidence for limited copies of the ovalbumin gene in chick oviduct. Proceedings of the National Academy of Sciences of the United States of America 70:3776–3780
    [Google Scholar]
  13. Jacobs-lorena M., Baglioni c., Borun T. W. 1972; Translation of messenger RNA for histones from HeLa cells by a cell-free extract from mouse ascites tumor. Proceedings of the National Academy of Sciences of the United States of America 69:2095–2099
    [Google Scholar]
  14. Kacian D. L., Spiegelman S., Bank A., Terada M., Metafora S., Dow L., Marks P. A. 1972; In Vitro synthesis of DNA components of human genes for globins. Nature New Biology 235:167–169
    [Google Scholar]
  15. Kacian D. L., Gambino R., Dow L., Grossbard E., Natta C., Ramirez F., Spiegelman S., Marks P. A., Bank A. 1973; Decreased globin messenger RNA in thalassemia detected by molecular hybridization. Proceedings of the National Academy of Sciences of the United States of America 70:1886–1890
    [Google Scholar]
  16. Kronenberg L. H. 1974; Cellular and molecular mechanisms controlling the interferon response. Ph.D. Thesis University of California, San Diego;
    [Google Scholar]
  17. Laskey R. A., Gurdon J. B., Crawford L. V. 1972; Translation of encephalomyocarditis viral RNA in oocytes of Xenopus laevis. Proceedings of the National Academy of Sciences of the United States of America 69:3665–3669
    [Google Scholar]
  18. Mach B., Faust C., Vassali P. 1973; Purification of 14S messenger RNA of immunoglobulin light chain that codes for a possible light-chain precursor. Proceedings of the National Academy of Sciences of the United States of America 70:451–455
    [Google Scholar]
  19. Moar V. A., Gurdon J. B., Lane C. D., Marbaix G. 1971; Translational capacity of living frog eggs and oocytes as judged by messenger RNA injection. Journal of Molecular Biology 61:93–103
    [Google Scholar]
  20. Orlova T. G., Georgadze J.J., Kognovitskaya A. I., Solovyov V. D. 1974; Interferon-inducing and interferon-translating functions of RNA isolated from Newcastle disease virus-infected cells. Acta Virologica 18:210–216
    [Google Scholar]
  21. Rhoads R. E., Mcknight G. s., Schimke R. T. 1973; Quantitative measurement of ovalbumin messenger ribonucleic acid activity. Journal of Biological Chemistry 248:2031–2039
    [Google Scholar]
  22. Rollins J. W., Flickinger R. A. 1972; Collagen synthesis in Xenopus oocytes after injection of nuclear RNA of frog embryo. Science, New York 178:1204–1205
    [Google Scholar]
  23. Ross J., Aviv H., scolnick E., Leder P. 1972; In vitro synthesis of DNA complementary to purified rabbit globin mRNA. Proceedings of the National Academy of Sciences of the United States of America 69:264–268
    [Google Scholar]
  24. Schechter I. 1973; Biologically and chemically pure mRNA coding for a mouse immunoglobulin L-chain prepared with the aid of antibodies and immobilized oligothymidine. Proceedings of the National Academy of Sciences of the United States of America 70:2256–2260
    [Google Scholar]
  25. Schutz G., Beato ?., Feigelson P. 1973; Messenger RNA for hepatic tryptophan oxygenase: its partial purification, its translation in a heterologous cell-free system, and its control by glucocorticoid hormones. Proceedings of the National Academy of Sciences of the United States of America 70:1218–1221
    [Google Scholar]
  26. Stevens R. H., Williamson A. R. 1972; Specific IgG mRNA molecules from myeloma cells in heterogeneous nuclear and cytoplasmic RNA containing poly-A. Nature, London 239:143–146
    [Google Scholar]
  27. Stewart W. E. 1974; Distinct molecular species of interferon. Virology 61:80–86
    [Google Scholar]
  28. Tan Y. H., Armstrong J. A., HO M. 1971; Intracellular interferon: kinetics of formation and release. Virology 45:837–840
    [Google Scholar]
  29. Taylor J. M., Schimke R. T. 1973; Synthesis of rat liver albumin in a rabbit reticulocyte cell-free protein- synthesizing system. Journal of Biological Chemistry 248:7661–7668
    [Google Scholar]
  30. Tomkins G. M., Levinson B. B., Baxter J. D., Dethlefsen L. 1972; Further evidence for post-transcriptional control of inducible tyrosine aminotransferase synthesis in cultured hepatoma cells. Nature New Biology 239:9–14
    [Google Scholar]
  31. Tuohimaa P., Segal S. J., Koide S. S. 1972; Induction of avidin synthesis by RNA obtained from chick oviduct. Proceedings of the National Academy of Sciences of the United States of America 69:2814–2817
    [Google Scholar]
  32. Verma I. M., Temple G. F., Fan H., Baltimore D. 1972; In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA. Nature New Biology 235:163–167
    [Google Scholar]
  33. Vilcek J., Ng M. H. 1971; Post-transcriptional control of interferon synthesis. Journal of Virology 7:588–594
    [Google Scholar]
  34. Watts R. L., Mathias A. P. 1967; The use of bentonite in the isolation of plant polyribosomes. Biochimica et Biophysica Acta 145:828–831
    [Google Scholar]
  35. Yamamoto Y., Tsukui K., Ohwaki M., Kawade Y. 1974; Electrophoretic characterization of purified mouse L cell interferon of high specific activity. Journal of General Virology 23:23–32
    [Google Scholar]
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