1887

Abstract

SUMMARY

Normal rat kidney cells infected by a variety of transformation-defective temperature-sensitive avian leukosis sarcoma virus mutants were tested for the expression of transformation characteristics at permissive and restrictive temperature. Morphology, growth behaviour and agglutinability by concanavalin A corresponded fully to the phenotype of the infected cells: at permissive temperature the cells resembled wild type virus transformed cells, whereas when grown under restrictive conditions they became virtually indistinguishable from normal cells.

The quantitative expression of allo- or xenogeneic cell surface antigens was not significantly affected by the phenotype of the cells. Two out of the five tested mutants induced tumour antigens in the expected temperature-dependent manner, whereas the other three mutants were able to induce tumour-specific cell surface antigens even in the revertant cells cultured at the restrictive temperature.

These findings extend previous results about tumour antigen induction in mutant-infected cells of the natural host, the chicken embryo fibroblasts. The value of transformation-defective tumour antigen-positive mutants for vaccination purposes will be discussed.

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1975-08-01
2024-05-01
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References

  1. Alexander P. 7972; Foetal ‘antigens’ in cancer. Nature, London 235:137–140
    [Google Scholar]
  2. Anderson H. R., Dresser W. D. 1971; The preparation and efficacy of purified anti-lymphocyte antibody and the effect of labelling this material with 125I. European Journal of Immunology 1:31–35
    [Google Scholar]
  3. Bauer H. 1974; Virion and tumour cell antigens of C-type RNA tumour viruses. Advances in Cancer Research 20:275–341
    [Google Scholar]
  4. Bauer H., Kurth R., Rohrschneider L., Pauli G., Friis R. R., Gelderblom H. 1975; On the role of cell surface changes in RNA-tumour virus transformed cells. Cold Spring Harbor Symposium of Quantitative Biology 39: (in the press)
    [Google Scholar]
  5. Beemon K., Duesberg P., Vogt P. K. 1974; Evidence for crossing-over betweenavian tumour viruses based on analysis of viral RNAs. Proceedings of the National Academy of Sciences of the United States of America 71:1254–1258
    [Google Scholar]
  6. Beveridge W. I. B. 1973; Immunity to cancer, Part I. Bulletin of the World Health Organization 49:81–91 Part II: Bulletin of the World Health Organization 49, 205–213
    [Google Scholar]
  7. Billeter M. A., Parsons J. T., Coffin J. M. 1974; The nucleotide sequence complexity of avian tumour virus RNA. Proceedings of the National Academy of Sciences of the United States of America 71:3560–3564
    [Google Scholar]
  8. Burger M. M., Martin G. S. 1972; Agglutination of cells transformed by Rous sarcoma virus by wheat germ agglutinin and concanavalin A. Nature New Biology 237:9–12
    [Google Scholar]
  9. Burger M. M. 1973; Surface changes in transformed cells detected by lectins. Federation Proceedings 32:91–101
    [Google Scholar]
  10. Duc-Nguyen J., Rosenblum E. M., Zeigel R. F. 1966; Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. Journal of Bacteriology 92:1133–1140
    [Google Scholar]
  11. Gelderblom H., Bauer H. 1973; Common avian oncornavirus-induced tumour antigens in different species as revealed by immunoferritin techniques. International Journal of Cancer 11:466–472
    [Google Scholar]
  12. Gelderblom H., Bauer H., Graf T. 1972; Cell surface antigens induced by avian RNA tumour viruses: detection by immunoferritin techniques. Virology 47:416–425
    [Google Scholar]
  13. Graf T., Bauer H., Gelderblom H., Bolognesi D. P. 1971; Studies on the reproductive and cell-converting abilities of avian sarcoma viruses. Virology 43:427–441
    [Google Scholar]
  14. Graf T., Friis R. R. 1973; Differential expression of transformation in rat and chicken cells infected with an avian sarcoma virus ts mutant. Virology 56:369–374
    [Google Scholar]
  15. Hellström K. E., Hellström I. i974; Lymphocyte mediated cytotoxicity and blocking serum activity to tumour antigens. Advances in Immunology 18:209–277
    [Google Scholar]
  16. Kawai S., Hanafusa H. 1971; The effects of reciprocal changes in temperature on the transformed state of cells infected with a Rous sarcoma virus mutant. Virology 46:470–479
    [Google Scholar]
  17. Klein G. 1966; Tumour antigens. Annual Review of Microbiology 20:223–252
    [Google Scholar]
  18. Kurth R., Bauer H. 1972a; Common tumour-specific surface antigens on cells of different species transformed by avian RNA tumour viruses. Virology 49:145–159
    [Google Scholar]
  19. Kurth R., Bauer H. 1972b; Cell surface antigens induced by RNA tumour viruses: detection by a cytotoxic microassay. Virology 47:426–433
    [Google Scholar]
  20. Kurth R., Bauer H. 1973a; Avian oncornavirus induced tumour antigens of embryonic and unknown origin. Virology 56:496–504
    [Google Scholar]
  21. Kurth R., Bauer H. 1973b; Influence of dibutyryl cyclic AMP and theophylline on cell surface antigens of oncornavirus transformed cells. Nature New Biology 243:243–245
    [Google Scholar]
  22. Kurth R., Bauer H. 1975; Avian RNA tumour viruses: a model for studying tumour associated cell surface alterations. Biochimica et Biophysica Acta Reviews on Cancer (in the press)
    [Google Scholar]
  23. Kurth R., Friis R. R., Wyke J. A., Bauer H. 1975; Expression of tumour specific surface antigens on cells infected with temperature-sensitive mutants of avian sarcoma virus. Virology (in the press)
    [Google Scholar]
  24. Martin G. S. 1970; Rous sarcoma virus: a function required for the maintenance of the transformed state. Nature, London 227:1021–1023
    [Google Scholar]
  25. Nossal G. J. V., Warner N. L., Lewis H., Sprent J. 1972; Quantitative features of a sandwich radio-immunolabelling technique for lymphocyte surface receptors. Journal of Experimental Medicine 135:405–428
    [Google Scholar]
  26. Pross H. F., Eidinger D. 1974; Antigenic competition: a review of non-specific antigen-induced suppression. Advances in Immunology 18:133–168
    [Google Scholar]
  27. Sjögren H. O., Hellström I., Bansal S. C., Hellström K. E. 1971; Suggestive evidence that the ‘blocking antibodies’ of tumour bearing individuals may be antigen-antibody complexes. Proceedings of the National Academy of Sciences of the United States of America 68:1372–1375
    [Google Scholar]
  28. Taussig M. J. 1974; Antigenic competition. Current Topics in Microbiology and Immunology 60:125–174
    [Google Scholar]
  29. Toyoshima K., Vogt P. K. 1969; Temperature-sensitive mutants of an avian sarcoma virus. Virology 39:930–931
    [Google Scholar]
  30. Wyke J. A. 1973; The selective isolation of temperature-sensitive mutants of Rous sarcoma virus. Virology 52:587–590
    [Google Scholar]
  31. Wyke J. A., Linial M. 1973; Temperature-sensitive avian sarcoma viruses: a physiological comparison of twenty mutants. Virology 53:152–161
    [Google Scholar]
  32. Wyke J. A., Bell J. G., Beamand J. A. 1975; Genetic recombination among temperature sensitive mutants of Rous sarcoma virus. Cold Spring Harbor Symposium on Quantitative Biology 39: (in the press)
    [Google Scholar]
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