1887

Abstract

SUMMARY

Pike fry rhabdovirus (PFR) RNA has been characterized as a single-stranded non-segmented molecule of about 4 × 10 daltons. In sucrose gradients in 0.1 -NaCl it has a sedimentation coefficient of 40 to 45 S, somewhat lower than that of Semliki Forest virus RNA. The sedimentation velocity of the RNA is strongly influenced by the salt concentration and divalent cations. It shows a buoyant density of 1.65 g/ml in caesium sulphate. The base composition of the RNA is 21.6% G, 25.1% A, 22.4% C and 30.9% U. It is thus comparable to the RNAs of other rhabdoviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-29-1-133
1975-10-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/29/1/JV0290010133.html?itemId=/content/journal/jgv/10.1099/0022-1317-29-1-133&mimeType=html&fmt=ahah

References

  1. Bishop D. H. L., Claybrook J. R., Spiegelman S. 1967; Electrophoretic separation of viral nucleic acid on polyacrylamide gels. Journal of Molecular Biology 26:373–387
    [Google Scholar]
  2. Boedtker H. 1968; Dependence of the sedimentation coefficient on molecular weight of RNA after reaction with formaldehyde. Journal of Molecular Biology 35:61–70
    [Google Scholar]
  3. Kinkelin De P., Le Berre M., Lenoir G. 1974; Rhabdovirus des poissons. I. Propriétés in vitro du virus de la maladie rouge de l’alevin de brochet. Annales de Microbiologie (Institut Pasteur) 125 A:93–111
    [Google Scholar]
  4. Davidson J. N., Smellie R. M. S. 1952; Phosphorus compounds in the cell. 3. The incorporation of radioactive phosphorus into the ribonucleotide fraction of liver tissue. Biochemical Journal 52:599–606
    [Google Scholar]
  5. Hearst J. E., Vinograd J. 1961; The net hydration of T-4 bacteriophage deoxyribonucleic acid and the effect of hydration on buoyant behaviour in a density gradient at equilibrium in the ultracentrifuge. Proceedings of the National Academy of Science of the United States of America 47:1005–1014
    [Google Scholar]
  6. Knudson D. L. 1973; Rhabdoviruses. Journal of General Virology 20: (supplement) 105–130
    [Google Scholar]
  7. Mccain B. B., Fryer J. L., Pilcher K. S. 1974; Physico-chemical properties of RNA of salmonid hematopoietic necrosis virus (Oregon strain). Proceedings of the Society for Experimental Biology and Medicine 146:630–634
    [Google Scholar]
  8. Peacock A. C., Dingman C. W. 1968; Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry 7:668–674
    [Google Scholar]
  9. Roy P., Clark H. F., Madore H. P., Bishop D. H. L. 1975; RNA Polymerase associated with virions of pike fry rhabdovirus. Journal of Virology 15:338–347
    [Google Scholar]
  10. Simmons D. T., Strauss J. H. 1972; Replication of Sindbis virus. I. Relative size and genetic content of 26S and 49S RNA. Journal of Molecular Biology 71:599–613
    [Google Scholar]
  11. Van Der Zeijst B. A. M., Bloemers H. P. J. 1975; 4698 Isokinetic glycerol and sucrose gradients for density gradient centrifugation. In Handbook of Biochemistry 3rd edition (in the press) Edited by Fasman G. D. Cleveland, Ohio, USA: The Chemical Rubber Company;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-29-1-133
Loading
/content/journal/jgv/10.1099/0022-1317-29-1-133
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error