1887

Abstract

SUMMARY

Infection of human embryonic fibroblast cell monolayers with neutral red and light-inactivated herpes simplex virus, type 2 (HSV-2) at supra-optimal temperature (42 °C) resulted in persistence of viable cells in suspension culture at 37 °C which have properties in common with virus transformed cells: formation of cell aggregates, HSV-2-specific antigens and colony formation in soft methyl cellulose medium. These data are consistent with the idea that photodynamic inactivated HSV-2 has potential oncogenic activity.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-30-2-257
1976-02-01
2024-05-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/30/2/JV0300020257.html?itemId=/content/journal/jgv/10.1099/0022-1317-30-2-257&mimeType=html&fmt=ahah

References

  1. Darai G., Munk K. 1973; Human embryonic lung cells abortively infected with herpes virus hominis type 2 show some properties of cell transformation. Nature New Biology 241:268–269
    [Google Scholar]
  2. Duff R., Rapp F. 1971; Properties of hamster embryo fibroblasts transformed in vitro after exposure to ultraviolet-irradiated herpes simplex virus type 2. Journal of Virology 8:469–477
    [Google Scholar]
  3. Dulbecco R., Hartwell L. H., Vogt M. 1965; Induction of cellular DNA synthesis by polyoma virus. Proceedings of the National Academy of Sciences of the United States of America 53:403–410
    [Google Scholar]
  4. Elber T. D. 1971; Photoinactivation may find use against herpesvirus. Journal of the American Medical Association 217:270–271
    [Google Scholar]
  5. Fox T. O., Levine A. J. 1971; Relationship between virus-induced cellular deoxyribonucleic acid synthesis and transformation by simian virus 40. Journal of Virology 7:473–477
    [Google Scholar]
  6. Friedrick E. G. 1973; Relief for herpes vulvitis. Obstetrics and Gynecology 41:74–77
    [Google Scholar]
  7. Gerber P., Hoyer B. H. 1971; Induction of cellular DNA synthesis in human leucocytes by Epstein-Barr virus. Nature, London 231:46–47
    [Google Scholar]
  8. Kaufman R. H., Gardner H. L., Brown D., Wallis C., Rawls W. E., Melnick J. L. 1973; Herpes genitalis treated by photodynamic inactivation of virus. American Journal of Obstetrics and Gynecology 117:1144–1146
    [Google Scholar]
  9. Kucera L., Gusdon J. P. jun 1975; Abstract. Transformation of human embryonic fibroblast cells with dye-light inactivated herpes simplex virus type 2. Gynecologic Investigations 6:42
    [Google Scholar]
  10. Kucera L., Simonson J. 1974; Isolation and characterization of a cell line from the cocultivation of Lucke renal tumor cells and nontransformed feeder cells. Journal of the National Cancer Institute 53:415–421
    [Google Scholar]
  11. Jui-Lien H. L., Jerkofsky M. A., Rapp F. 1975; Demonstration of oncogenic potential of mammalian cells transformed by DNA-containing viruses following photodynamic inactivation. International Journal of Cancer 15:190–202
    [Google Scholar]
  12. Macnab J. C. M. 1974; Transformation of rat embryo cells by temperature-sensitive mutants of herpes simplex virus. Journal of General Virology 24:143–153
    [Google Scholar]
  13. Macpherson I., Montagnier L. 1964; Agar suspension culture for the selective assay of cells transformed by polyoma virus. Virology 23:291–294
    [Google Scholar]
  14. Marcon M., Kucera L. S. 1975; The effect of temperature on DNA synthesis and infectious virus production in herpes simplex virus type 2 (HSV-2) infected human cells. Abstracts of the Annual Meeting of the American Society for Microbiology-1975 p 239
    [Google Scholar]
  15. Melvin P., Kucera L. S. 1975; Induction of human cell DNA synthesis by herpes simplex virus type 2. Journal of Virology 15:534–539
    [Google Scholar]
  16. Rapp F., Duff R. 1974; Oncogenic conversion of normal cells by inactivated herpes simplex viruses. Cancer 34:1353–1362
    [Google Scholar]
  17. Rapp F., Jui-Lien H. L., Jerkofsky M. 1973; Transformation of mammalian cells by DNA-containing virus following photodynamic inactivation. Virology 55:339–345
    [Google Scholar]
  18. St Jeor S. C., Albrecht T. B., Funk F. D., Rapp F. 1794; Stimulation of cellular DNA synthesis by human cytomegalovirus. Journal of Virology 13:353–362
    [Google Scholar]
  19. Takahashi M., Yamanishi K. 1974; Transformation of hamster embryo and human embryo cells by temperature sensitive mutants of herpes simplex virus type 2. Virology 61:306–311
    [Google Scholar]
  20. Wallis C., Melnick J. L. 1964; Irreversible photosensitization of viruses. Virology 23:520–527
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-30-2-257
Loading
/content/journal/jgv/10.1099/0022-1317-30-2-257
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error