1887

Abstract

SUMMARY

During studies on the mechanisms of virus latency, reactivation and resultant tissue injury in mice infected with murine cytomegalovirus (MCMV) or at birth, we found the occurrence of three distinct pathological groups. In the first group, mice died within 4 weeks of exposure to virus and showed evidence of tissue injury due to MCMV in multiple tissues and organs of the body. The second group consisted of mice which survived the initial infection and was composed of a minority (about 25%) which shed virus (chronically infected). The third group (about 75%) consisted of mice in which shedding of virus could not be detected (latently infected). Study of the latter group indicated that virus was not detected in brain, thymus, liver, kidneys, urine or serum by co-cultivation techniques or by cellular DNA-MCMV DNA hybridization. In contrast, virus could be activated from spleen cells by co-cultivation with allogenic but not syngeneic feeder cells and MCMV-DNA was detected in amounts equivalent to 3 to 4 virus genomes per 100 spleen cells. In both the latently infected and chronically infected mice, in all strains studied evidence of virus-antivirus immune complex deposits in the renal glomeruli occurred. Only one of the six infected strains (C57 Br/cdJ) studied showed manifestations of autoimmune disease with the formation of antibodies to nuclear antigens, DNA and soluble nucleoprotein.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-33-2-267
1976-11-01
2024-05-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/33/2/JV0330020267.html?itemId=/content/journal/jgv/10.1099/0022-1317-33-2-267&mimeType=html&fmt=ahah

References

  1. Britten R. J., Kohne D. E. 1968; Repeated sequences in DNA. Science, New York 161:529–540
    [Google Scholar]
  2. Cannat A., Varet B. 1972; Induction of antinuclear antibodies in (C × B6) F1 mice inoculated with Graffi and Rauscher leukemogenic viruses (36936). Proceedings of the Society for Exeprimental Biology and Medicine 141:1077–1080
    [Google Scholar]
  3. Caul E. O., Clarke S. K. R., Mott M. G., Perham T. G. M., Wilson R. S. E. 1971; Cytomegalovirus infections after open heart surgery. Lancet 1:777–781
    [Google Scholar]
  4. Craighead J. E., Hanshaw J. B., Carpenter C. B. 1967; Cytomegalovirus infection after renal allotransplantation. Journal of the American Medical Association 201:725–728
    [Google Scholar]
  5. Crawford L. V. 1969; Purification of polyoma virus. In Fundamental Techniques in Virology pp 75–81 Edited by Habel K., Saltzman N. New York: Academic Press;
    [Google Scholar]
  6. Croker B. P., Del-Villano B. C., Jensen F. C., Lerner R. A., Dixon F. J. 1974; Immunopathogenicity and oncogenicity of murine leukemia viruses. I. Induction of immunologic disease and lymphoma in (BALB/c × NZB) F, mice by Scripps leukemia virus. Journal of Experimental Medicine 140:1028–1048
    [Google Scholar]
  7. Dixon F. J., Tonietti G., Oldstone M. B. A., Aoki T., McConahey P. J. 1971; The effect of chronic viral infections on NZ mice. In Sixth International Symposium of Immunopathology pp 377–384 Edited by Miescher P. Basel: Schwabe & Co;
    [Google Scholar]
  8. Duwall C. P., Casazza A. R., Grimley P. M., Carbone P. P., Rowe W. P. 1966; Recovery of cytomegalovirus from adults with neoplastic disease. Annals of Internal Medicine 64:531–541
    [Google Scholar]
  9. Fenner F., McAuslan B. R., Mims C. A., Sambrook J., White D. O. 1974 In The Biology of Animal Viruses 2nd edition New York: Academic Press;
    [Google Scholar]
  10. Kaariainen L., Paloheimo J., Klemola E., Makela T., Koivuneimi A. 1966; Cygomegalovirus-mono-nucleosis: isolation of the virus and demonstration of subclinical infections after fresh blood transfusions in connection with open heart surgery. Annales Medicinae Experimentalis et Biologiae Fenniae 44:297–301
    [Google Scholar]
  11. Kantor G. L., Goldbey L. S., Johnson B. L. 1970; Immunological abnormalities induced by postperfusion cytomegalovirus infection. Annals of Internal Medicine 73:553–558
    [Google Scholar]
  12. Kim K. S., Carp R. L. 1971; Growth of murine cytomegalovirus in various cell lines. Journal of Virology 7:720–725
    [Google Scholar]
  13. Kingsbury D. T., Lerner R. A. 1974; Encapsulation of lymphocyte DNA by vesicular stomatitis virus. Proceedings of the National Academy of Sciences of the United States of America 71:1753–1757
    [Google Scholar]
  14. Kohne D. E. 1968; Isolation and characterization of bacterial ribosomal RNA cistrons. Biophysical Journal 7:1104–1118
    [Google Scholar]
  15. Krech U. H., Jung M., Jung F. 1971 In Cytomegalovirus Infections in Man pp 36–39 Basel: S. Karger, AG;
    [Google Scholar]
  16. Lang D. J., Scolnick E. M., Willerson J. T. 1968; Association of cytomegalovirus infection with the’post perfusion syndrome. New England Journal of Medicine 278:1147–1149
    [Google Scholar]
  17. Mancini G., Carbonara A. O., Heremans J. F. 1965; Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 2:235–254
    [Google Scholar]
  18. McCordock H. A., Smith M. G. 1936; The visceral lesions produced in mice by the salivary gland virus of mice. Journal of Experimental Medicine 63:303–310
    [Google Scholar]
  19. McCracken G. H. Jun, Shinefield H. R., Cobb K. 1969; Congenital cytomegalic inclusion disease: a longitudinal study of 20 patients. American Journal of Diseases of Children 117:522–539
    [Google Scholar]
  20. Müller-Eberhard H. J. 1960; Anew supporting medium for preparative electrophoresis. Scandinavian Journal of Clinical and Laboratory Investigation 12:33–37
    [Google Scholar]
  21. Northway J. D., Tan E. M. 1972; Differentiation of antinuclear antibodies giving speckled staining patterns in immunofluorescence. Clinical Immunology and Immunopathology 1:140–154
    [Google Scholar]
  22. Olding L. B., Jensen F. C., Oldstone M. B. A. 1975; Pathogenesis of cytomegalovirus infection. I. Activation of virus from bone marrow derived lymphocytes by in vitro allogenic reaction. Journal of Experimental Medicine 141:561–572
    [Google Scholar]
  23. Oldstone M. B. A. 1975; Virus neutralization and virus induced immune complex disease: virus-antibody union resulting in immunoprotection or immunologic injury – two different sides of the same coin. In Progress in Medical Virology vol 19 pp 84–119 Edited by Melnick J. L. Basel: S. Karger;
    [Google Scholar]
  24. Oldstone M. B. A., Del Villano B. C., Dixon F. J. 1976; Autologous immune responses to the major oncornavirus polypeptides in unmanipulated AKR/J mice. Journal of Virology (in the press)
    [Google Scholar]
  25. Oldstone M. B. A., Dixon F. J. 1969; Pathogenesis of chronic disease associated with persistent lymphocytic choriomeningitis viral infection. I. Relationship of antibody production to disease in neonatally infected mice. Journal of Experimental Medicine 129:483–505
    [Google Scholar]
  26. Oldstone M. B. A., Dixon F. J. 1971a; Acute viral infection: tissue injury mediated by anti-viral antibody through a complement effector system. Journal of Immunology 107:1274–1280
    [Google Scholar]
  27. Oldstone M. B. A., Dixon F. J. 1971b; Virus anti-viral antibody complexes. In Progress in Immunology pp 763–777 Edited by Amos D. B. New York: Academic Press;
    [Google Scholar]
  28. Oldstone M. B. A., Dixon F. J. 1972; Inhibition of antinuclear and anti-DNA antibody responses in New Zealand mice chronically infected with lactic dehydrogenase virus. Science, New York 175:784–786
    [Google Scholar]
  29. Randall C., Gafford L. 1969; Extraction of high molecular weight viral DNA. In Fundamental Techniques in Virology pp 483–486 Edited by Habel K., Solzman N. New York: Academic Press;
    [Google Scholar]
  30. Robitaille P., Tan E. M. 1973; Relationship between deoxyribonucleo-protein and deoxyribonucleic acid antibodies in systemic lupus erythematosus. Journal of Clinical Investigation 52:316–323
    [Google Scholar]
  31. Ruebner B. H., Hirano T., Slusser R., Osborn J., Medearis D. N. Jun 1966; Cytomegalovirus infection. Viral ultrastructure with particular reference to the relationship of lysosomes to cytoplasmic inclusions. American Journal of Pathology 48:971–990
    [Google Scholar]
  32. Schwartz J. N., Daniels C. A., Klintworth G. K. 1975; Lymphoid cell necrosis, thymic atrophy, and growth retardation in newborn mice inoculated with murine cytomegalovirus. American Journal of Pathology 79:509–518
    [Google Scholar]
  33. Selgrade M., Osborn J. 1974; Role of macrophages in resistance to murine cytomegalovirus. Infection and Immunity 10:1383–1390
    [Google Scholar]
  34. Stern H., Elek S. D., Booth J. C., Fleek D. G. 1969; Microbial causes of mental retardation. The role of prenatal infections with cytomegalovirus, rubella virus, and toxoplasma. Lancet 11:443–448
    [Google Scholar]
  35. Tan E. M. 1967; An immunologic precipitating system between soluble nucleoprotein and serum antibody in systemic lupus erythematosus. Journal of Clinical Investigation 46:735–745
    [Google Scholar]
  36. Tonietti G., Oldstone M. B. A., Dixon F. J. 1970; The effect of induced chronic viral infections on the immunologic diseases of New Zealand mice. Journal of Experimental Medicine 132:89–109
    [Google Scholar]
  37. Weller T. H., Hanshaw J. B. 1962; Virological and clinical observations on cytomegalic inclusion disease. New England Journal of Medicine 226:1233–1244
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-33-2-267
Loading
/content/journal/jgv/10.1099/0022-1317-33-2-267
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error