1887

Abstract

SUMMARY

The synthesis of proteins has been studied in tobacco protoplasts infected with cowpea chlorotic mottle virus (CCMV). Three new proteins were found with mol. wt. 19000 (P1), 34000 to 36000 (P2) and 100000 (P3). Protein P2 reached a maximum steady level about 16 to 20 h after infection; it was not a precursor of P1, which is virus coat protein. P3 appeared later than the other two proteins, reached its maximum about 44 h after infection and then declined. P2 and P3 were localized in the 1000 and 20000 pellets of a cell homogenate, in contrast to P1 (coat) which was largely found in the supernatant fraction after centrifuging at 20000 for 30 min. The three proteins account for about 60% of the tripartite genome of CCMV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-34-2-285
1977-02-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/34/2/JV0340020285.html?itemId=/content/journal/jgv/10.1099/0022-1317-34-2-285&mimeType=html&fmt=ahah

References

  1. Bancroft J. B., Lane L. C. 1973; Genetic analysis of cowpea chlorotic mottle and brome mosaic viruses. Journal of General Virology 19:381–389
    [Google Scholar]
  2. Bancroft J. B., Motoyoshi F., Watts J. W., Dawson J. R. O. 1975; Cowpea chlorotic mottle and brome mosaic viruses in tobacco protoplasts. 2nd John Innes Symposium: Modification of the Information Content of Plant Cells pp 133–160 Amsterdam: North Holland Publishing Co;
    [Google Scholar]
  3. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  4. Davies J. W., Kaesberg P. 1974; Translation of virus mRNA: protein synthesis directed by several virus RNAs in a cell-free extract from wheat germ. Journal of General Virology 25:11–20
    [Google Scholar]
  5. Goodman D., Matzura H. 1971; An improved method of counting radioactive acrylamide gels. Analytical Biochemistry 42:481–486
    [Google Scholar]
  6. Hariharasubramanian V., Hadidi A., Singer B., Fraenkel-Conrat H. 1973; Possible identification of a protein in brome mosaic virus infected barley as a component of viral RNA polymerase. Virology 54:190–198
    [Google Scholar]
  7. Motoyoshi F., Bancroft J. B., Watts J. W., Burgess J. 1973a; The infection of tobacco protoplasts with cowpea chlorotic mottle virus and its RNA. Journal of General Virology 20:177–193
    [Google Scholar]
  8. Motoyoshi F., Bancroft J. B., Watts J. W. 1973b; A direct estimate of the number of cowpea chlorotic mottle virus particles absorbed by tobacco protoplasts that become infected. Journal of General Virology 21:159–161
    [Google Scholar]
  9. Motoyoshi F., Watts J. W., Bancroft J. B. 1974; Factors influencing the infection of tobacco protoplasts by cowpea chlorotic mottle virus. Journal of General Virology 25:245–256
    [Google Scholar]
  10. Paterson R., Knight C. A. 1975; Protein synthesis in tobacco protoplasts infected with tobacco mosaic virus. Virology 64:10–22
    [Google Scholar]
  11. Sakai F., Takebe I. 1972; A non-coat protein synthesized in tobacco mesophyll protoplasts infected by tobacco mosaic virus. Molecular and General Genetics 118:93–96
    [Google Scholar]
  12. Sakai F., Takebe I. 1974; Protein synthesis in tobacco mesophyll protoplasts induced by tobacco mosaic virus infection. Virology 62:426–433
    [Google Scholar]
  13. Takebe L. 1975; The use of protoplasts in plant virology. Annual Review of Phytopathology 13:105–125
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-34-2-285
Loading
/content/journal/jgv/10.1099/0022-1317-34-2-285
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error