1887

Abstract

SUMMARY

Treatment of purified preparations of tobacco rattle virus with 2 m-EDTA decreased their infectivity, and that of RNA extracted from them, by about 90%. The effect of EDTA was apparently to remove divalent metal ions from the virus particles, making the RNA inside accessible to attack by a ribonuclease present in the preparations. Provided the enzyme activity was inhibited while the ions were absent, they could be put back with full restoration of full stability of infectivity.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-34-3-547
1977-03-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/34/3/JV0340030547.html?itemId=/content/journal/jgv/10.1099/0022-1317-34-3-547&mimeType=html&fmt=ahah

References

  1. Cooper J. I., Mayo M. A. 1972; Some properties of the particles of three tobravirus isolates. Journal of General Virology 16:285–297
    [Google Scholar]
  2. Ehrenberg L., Fedorcsak I., Solymosy F. 1976; Diethyl pyrocarbonate in nucleic acid research. Progress in Nucleic Acid Research and Molecular Biology 16:189–262
    [Google Scholar]
  3. Govier D. A., Woods R. D. 1971; Changes induced by magnesium ions in the morphology of some plant viruses with filamentous particles. Journal of General Virology 13:127–132
    [Google Scholar]
  4. Gray J. C. 1974; The inhibition of ribonuclease activity and the isolation of polysomes from leaves of the French bean, Phaseolus vulgaris. Archives of Biochemistry and Biophysics 163:343–348
    [Google Scholar]
  5. Harrison B. D., Kubo S., Robinson D. J., Hutcheson A. M. 1976; The multiplication cycle of tobacco rattle virus in tobacco mesophyll protoplasts. Journal of General Virology 33:237–248
    [Google Scholar]
  6. Harrison B. D., Nixon H. L. 1959; Separation and properties of particles of tobacco rattle virus with different lengths. Journal of General Microbiology 2:569–581
    [Google Scholar]
  7. Hsu C. H., Sehgal O. P., Pickett E. E. 1976; Stabilizing effect of divalent metal ions on virions of southern bean mosaic virus. Virology 69:587–595
    [Google Scholar]
  8. Kubo S., Harrison B. D., Robinson D. J., Mayo M. A. 1975; Tobacco rattle virus in tobacco mesophyll protoplasts: infection and virus multiplication. Journal of General Virology 27:293–304
    [Google Scholar]
  9. Lienhard G. E., Secemski I. I., Koehler K. A., Lindquist R. N. 1971; Enzymatic catalysis and the transition state theory of reaction rates: transition state analogs. Cold Spring Harbor Symposium on Quantitative Biology 36:45–51
    [Google Scholar]
  10. Wells J. M., Sisler H. D. 1969; The effect of EDTA and Mg2+ on the infectivity and structure of southern bean mosaic virus. Virology 37:227–236
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-34-3-547
Loading
/content/journal/jgv/10.1099/0022-1317-34-3-547
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error