1887

Abstract

SUMMARY

Chinese hamster kidney cells are semi-permissive to simian virus 40 (SV40). Exposure to mitomycin C (MC) of Chinese hamster kidney cells infected with SV40 DNA enhanced the yield of infectious virus 10- to 100-fold. This stimulation occurred whether the treatment was performed before or after infection. A simultaneous increase in the number of V antigen-synthesizing cells and virus-producing cells, as well as the virus burst size, was observed upon MC pretreatment, whereas the proportion of T antigen-synthesizing cells remained unchanged. MC pretreatment clearly stimulated virus DNA replication in SV40 virus-infected cells. Cells treated with MC exhibited an unbalanced growth pattern, with continuing protein synthesis in the absence of cell division and a markedly reduced ability to replicate the cellular DNA. These results suggest that MC enhances the permissiveness of Chinese hamster kidney cells by inducing the synthesis of a specific cellular factor(s) required for SV40 replication in these cells. Exposure to ultraviolet light also enhanced infectious virus production in Chinese hamster kidney cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-36-1-137
1977-07-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/36/1/JV0360010137.html?itemId=/content/journal/jgv/10.1099/0022-1317-36-1-137&mimeType=html&fmt=ahah

References

  1. Burns W. H., Black P. H. 1968; Analysis of simian virus 40-induced transformation of hamster kidney tissue in vitro. V. Variability of virus recovery from cell clones inducible with mitomycin C and cell fusion. Journal of Virology 2:606–609
    [Google Scholar]
  2. Burns W. H., Black P. H. 1969; Analysis of SV40-induced transformation of hamster kidney tissue in vitro. VI. Characteristics of mitomycin C induction. Virology 39:625–634
    [Google Scholar]
  3. Fogel M. 1972; Induction of virus synthesis in polyoma-transformed cells by DNA antimetabolites and by irradiation after pretreatment with 5-bromodeoxyuridine. Virology 49:12–22
    [Google Scholar]
  4. Fogel M. 1973; Induction of polyoma virus by fluorescent (visible) light in polyoma-transformed cells pretreated with 5-bromodeoxyuridine. Nature New Biology 241:182–184
    [Google Scholar]
  5. Fogel M. 1975; Polyoma virus-transformed rat cell lines inducible for viral capsid antigen synthesis. Virology 65:446–454
    [Google Scholar]
  6. Fogel M., Sachs L. 1970; Induction of virus synthesis in polyoma-transformed cells by ultraviolet light and mitomycin C. Virology 42:251–256
    [Google Scholar]
  7. Gerber P. 1964; Virogenic hamster tumour cells: induction of virus synthesis. Science 145:833
    [Google Scholar]
  8. Graessmann A., Graessmann M., Mueller C. 1976; Regulatory mechanisms of simian virus 40 gene expression in permissive and in nonpermissive cells. Journal of Virology 17:854–858
    [Google Scholar]
  9. Hirai K., Lehman J., Defendi V. 1971; Integration of simian virus 40 deoxyribonucleic acid into the deoxyribonucleic acid of primary infected Chinese hamster cells. Journal of Virology 8:708–715
    [Google Scholar]
  10. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  11. Jensen F. C., Girardi A. J., Gilden R. V., Koprowski H. 1964; Infection of human and simian tissue cultures with Rous sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 52:53–59
    [Google Scholar]
  12. Kaplan J. C., Wilbert S. M., Black P. H. 1972; Analysis of simian virus 40-induced transformation of hamster kidney tissue in vitro. VIII. Induction of infectious simian virus 40 from virogenic transformed hamster cells by amino acid deprivation or cycloheximide treatment. Journal of Virology 9:448–453
    [Google Scholar]
  13. Kaplan J. C., Wilbert S. M., Collins J. J., Rakusanova T., Zamansky G. B., Black P. H. 1975; Isolation of simian virus 40-transformed inbred hamster cell lines heterogeneous for virus induction by chemicals or radiations. Virology 68:200–214
    [Google Scholar]
  14. Kit S., Kurimura T., Salvi M. L., Dubbs D. R. 1968; Activation of infectious SV40 DNA synthesis in transformed cells. Proceedings of the National Academy of Sciences of the United States of America 60:1239–1246
    [Google Scholar]
  15. Lavialle Ch., Stevenet J., Morris A. G., Suarez H. G., Estrade S., Salomon J. C., Cassingena R. 1975; Simian virus 40-Chinese hamster kidney cell interaction. I. Relationship of chromosome changes to transformation. Archives of Virology 49:127–139
    [Google Scholar]
  16. Lavialle Ch., Suarez H. G., Morris A. G., Estrade S., Stevenet J., Cassingena R. 1976; Simian virus40-Chinese hamster kidney cell interaction. II. The semipermissivity of the cell system. Archives of Virology 50:137–146
    [Google Scholar]
  17. Margalith M., Margalith E., Nasialski T., Goldblum N. 1970; Induction of simian virus 40 antigen in BSC-1 transformed cells. Journal of Virology 5:305–308
    [Google Scholar]
  18. Morris A. O., Lavialle Ch., Suarez H. G., Cassingena R. 1975; The induction of SV40-transformed Chinese hamster and mouse kidney cells by mitomycin C. Intervirology 5:305–312
    [Google Scholar]
  19. Morris A. G., Lavialle Ch., Suarez H. G., Stevenet J., Estrade S., Cassingena R. 1977; Simian virus40-Chinese hamster kidney cell interaction. III. Characteristics of chemical induction in a clone of virongenic transformed cells. Journal of General Virology 36:123–135
    [Google Scholar]
  20. Rakusanova T., Kaplan J. C., Smales W. P., Black P. H. 1976; Excision of viral DNA from host cell DNA after induction of simian virus 40-transformed hamster cells. Journal of Virology 19:279–285
    [Google Scholar]
  21. Rothschild H., Black P. H. 1970; Analysis of SV40-induced transformation of hamster kidney tissue in vitro. VII. Induction of SV40 virus from transformed hamster cell clones by various agents. Virology 42:251–256
    [Google Scholar]
  22. Staal S. P., Rowe W. P. 1975; Enhancement of adenovirus infection in WI-38 and AGMK cells by pretreatment of cells with 5-iodo-2′-deoxyuridine. Virology 64:513–519
    [Google Scholar]
  23. Suarez H. G., Cassingena R., Estrade S., Wicker R., Lavialle Ch., Lazar P. 1974; Properties of SV40 rescued from actinomycin D-sensitive and actinomycin D-resistant transformed hamster cells. I. Lytic infection. Archiv filr die gesamte Virusforschung 46:93–104
    [Google Scholar]
  24. Suarez H. G., Morris A. G., Lavialle Ch., Cassingena R. 1976; Enhanced SV40-virus replication in Chinese hamster kidney cells pretreated with 5-iodo-2′-deoxyuridine. Archives of Virology 50:249–253
    [Google Scholar]
  25. Tournier P., Cassingena R., Wicker R., Coppey J., Suarez H. G. 1967; Etude du mecanisme de l’induction chez des cellules de hamster Syrien transformees par le virus SV40. I. Proprietes d’une lignee cellulaire clonale. International Journal of Cancer 2:117–132
    [Google Scholar]
  26. Wicker R., Avrameas S. 1969; Localization of virus antigens by enzyme-labelled antibodies. Journal of General Virology 4:465–471
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-36-1-137
Loading
/content/journal/jgv/10.1099/0022-1317-36-1-137
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error