1887

Abstract

SUMMARY

Mol. wt. estimates have been derived for the covalently-closed circular DNAs extracted from seven nuclear polyhedrosis and three granulosis viruses by a comparison of their contour length with that of the replicative form of f1 bacteriophage DNA. The length of the circular DNA molecules was uniform for each of the ten viruses studied. The mol. wt. values obtained ranged from 58 × 10 to 94 × 10 although all but two values were within the range 69 × 10 to 81 × 10. There appeared to be two major size classes within the latter range. The mol. wt. of the three granulosis virus DNAs were from 68 × 10 to 71 × 10 while the nuclear polyhedrosis virus DNAs spanned the full range. Thus, the distribution of mol. wt. values obtained from covalently-closed DNAs isolated from baculoviruses was not consistent with the division of the group into nuclear polyhedrosis and granulosis viruses.

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1977-12-01
2024-05-02
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References

  1. Bellett A. J. D. 1969; Relationships among the polyhedrosis and granulosis viruses of insects. Virology 37:117–123
    [Google Scholar]
  2. Berkowitz S. A., Day L. A. 1974; Molecular weight of single-stranded fd bacteriophage DNA. High speed equilibrium sedimentation and light scattering measurements. Biochemistry 13:4825–4831
    [Google Scholar]
  3. Bode V. C., Machattie L. A. 1968; Electron microscopy of superhelical circular A DNA. Journal of Molecular Biology 32:673–679
    [Google Scholar]
  4. Davis R. W., Simon M., Davidson N. 1971; Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. In Methods in Enzymology, XXID pp 413–428 Edited by Grossman L., Moldave K. New York and London: Academic Press;
    [Google Scholar]
  5. Dawid I. B., Wolstenholme D. R. 1967; Ultracentrifuge and electron microscope studies on the structure of mitochondrial DNA. Journal of Molecular Biology 28:233–245
    [Google Scholar]
  6. Fenner F. 1976; The classification and nomenclature of viruses. Journal of General Virology 31:463–470
    [Google Scholar]
  7. Freifelder D. 1970; Molecular weights of coliphages and coliphage DNA IV. Molecular weights of DNA from bacteriophage T4, T5 and T7 and the general problem of determination of M. Journal of Molecular Biology 54:567–577
    [Google Scholar]
  8. Harrap K. A., Longworth J. F. 1974; An evaluation of purification methods for baculoviruses. Journal of Invertebrate Pathology 24:55–62
    [Google Scholar]
  9. Harrap K. A., Payne C. C., Robertson J. S. 1977; The properties of three baculoviruses from closely related hosts. Virology 79:14–31
    [Google Scholar]
  10. Helinski D. R., Clewell D. B. 1971; Circular DNA. Annual Review of Biochemistry 40:899–942
    [Google Scholar]
  11. Inman R. B., Schnos M. 1970; Partial denaturation of thymine- and 5-bromouracil-containing A DNA in alkali. Journal of Molecular Biology 49:93–98
    [Google Scholar]
  12. Knudson O. L. 1975; Replication of nuclear polyhedrosis viruses in cell cultures. Ph,. D. thesis University of Oxford.;
    [Google Scholar]
  13. Kok I. P., Chistiakova A. V., Gudz-Gorban A. P., Solomo A. P. 1968; Infectivity and structure of DNA of virus of nuclear polyhedrosis virus of the silkworm. Abstracts of the 13th International Congress for Entomology Moscow: p 127
    [Google Scholar]
  14. Lang D., Bujard H., Wolff B., Russell D. 1967; Electron microscopy of size and shape of viral DNA in solutions of different ionic strengths. Journal of Molecular Biology 23:163–181
    [Google Scholar]
  15. Marvin D. A., Hohn B. 1968; Filamentous bacterial viruses. Bacteriological Reviews 33:172–209
    [Google Scholar]
  16. Murphy T. F., Mccarthy W. J. 1976; Characterization of NPV DNAs. In Proceedings of the First International Colloquium on Invertebrate Pathology and IXth Annual meeting of the Society for Invertebrate Pathology p 329 Queens University; Kingston, Ontario.:
    [Google Scholar]
  17. O’neill R., Wetherill G. B. 1971; The present state of multiple comparison methods. Journal of the Royal Statistical Society 32:218–250
    [Google Scholar]
  18. Pinnock D. E. 1975; Pest populations and virus dosage in relation to crop productivity. In Baculoviruses for Insect Pest ControlSafety Considerations pp 147–157 Edited by Summers M., Engler R., Falcon L. A., Vail P. Washington: American Society for Microbiology;
    [Google Scholar]
  19. Radloff R., Bauer W., Vinograd J. 1967; A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proceedings of the National Academy of Sciences in the United States of America 57:1514–1521
    [Google Scholar]
  20. Summers M. D., Anderson D. L. 1972a; Granulosis virus deoxyribonucleic acid: a closed, double-stranded molecule. Journal of Virology 9:710–713
    [Google Scholar]
  21. Summers M. D., Anderson D. L. 1972b; Characterization of deoxyribonucleic acid isolated from the granulosis viruses of the cabbage looper, Trichoplusia ni and the fall armyworm, Spodoptera frugiperda . Virology 50:459–471
    [Google Scholar]
  22. Summers M. D., Anderson D. L. 1973; Characterization of nuclear polyhedrosis virus DNAs. Journal of Virology 12:1336–1346
    [Google Scholar]
  23. Wellauer P., Weber R., Wyler T. 1973; Electron microscopical study on the influence of the preparative conditions on contour length and structure of mitochondrial DNA of mouse liver. Journal of Ultrastructure Research 42:377–393
    [Google Scholar]
  24. Younghusband H. B., Inman R. B. 1974; The electronmicroscopy of DNA. Annual Review of Biochemistry 43605–619
    [Google Scholar]
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