1887

Abstract

SUMMARY

Purified preparations of isolate C of andean potato mottle virus (APMV) contained isometric particles 28 nm in diam. with sedimentation coefficients ( ) of about 53, and 93 and 112S. The two nucleoprotein components, middle (M) and bottom (B), were serologically indistinguishable, and each contained similar relative amounts of two polypeptide species, of mol. wt. 22100 and 41800. M particles had a density in CsCl of 1.41 g/ml, contained a single RNA species of mol. wt. 1.4 × 10 and stained differently from B particles with uranyl formate; B particles had a density of 1.46 g/ml and contained a single RNA species of mol. wt. 2.0 × 10. Preparations of M and B particles were much less infective when inoculated separately than in mixtures. In these properties APMV resembles other comoviruses. The present cryptogram of APMV is R/1:1.4/(27) + 2.0/(34):S/S:S/*, comovirus group.

The two isolates studied, C and H, infected systemically, and therefore differ from the type strain of APMV. Isolate C was antigenically indistinguishable from the type strain and produced similar symptoms in several solanaceous species. Isolate H differed antigenically from C and the type strain, and also differed somewhat in symptomatology and host range.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-39-1-171
1978-04-01
2024-05-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/39/1/JV0390010171.html?itemId=/content/journal/jgv/10.1099/0022-1317-39-1-171&mimeType=html&fmt=ahah

References

  1. Barnett O. W., Murant A. F. 1970; Host range, properties and purification of raspberry bushy dwarf virus. Annals of Applied Biology 65:435–449
    [Google Scholar]
  2. Chervenka C. H. 1969 A manual of methods for the analytical ultracentrifuge Palo Alto; Spinco Division of Beckman Instruments, Inc:
    [Google Scholar]
  3. Crowther R. A., Geelen J. L. M. C., Mellema J. E. 1974; A three-dimensional image reconstruction of cowpea mosaic virus. Virology 57:20–27
    [Google Scholar]
  4. Diener T. O., Schneider I. R. 1966; The two components of tobacco ringspot virus nucleic acid: origin and properties. Virology 29:100–105
    [Google Scholar]
  5. Fribourg C. E., Jones R. A. C., Koenig R. 1977; Andean potato mottle, a new member of the cowpea mosaic virus group. Phytopathology 67:969–974
    [Google Scholar]
  6. Geelen J. L. M. C., Van Kammen A., Verduin B. J. M. 1972; Structure of the capsid of cowpea mosaic virus. The chemical subunit: molecular weight and number of subunits per particle. Virology 49:205–213
    [Google Scholar]
  7. Harrison B. D., Finch J. T., Gibbs A. J., Hollings M., Shepherd R. J., Valenta V., Wetter C. 1971; Sixteen groups of plant viruses. Virology 45:356–363
    [Google Scholar]
  8. Markham R. 1960; A graphical method for the rapid determination of sedimentation coefficients. Biochemical Journal 77:516–519
    [Google Scholar]
  9. Mayo M. A., Harrison B. D., Murant A. F., Barker H. 1973; Cross-linking of RNA induced by ultraviolet irradiation of particles of raspberry ringspot virus. Journal of General Virology 19:155–159
    [Google Scholar]
  10. Mayo M. A., Murant A. F., Harrison B. D., Goold R. A. 1974; Two protein and two RNA species in particles of strawberry latent ringspot virus. Journal of General Virology 24:29–37
    [Google Scholar]
  11. Murant A. F., Mayo M. A., Harrison B. D., Goold R. A. 1972; Properties of virus and RNA components of raspberry ringspot virus. Journal of General Virology 16:327–338
    [Google Scholar]
  12. Reijnders L., Aalbers A. M. J., Van Kammen A., Thuring R. W. J. 1974; Molecular weights of plant viral RNAs determined by gel electrophoresis under denaturing conditions. Virology 60:515–521
    [Google Scholar]
  13. Reijnders L., Sloof P., Sival J., Borst P. 1973; Gel electrophoresis of RNA under denaturing conditions. Biochemica et Biophysica Acta 324:320–333
    [Google Scholar]
  14. Taylor R. H., Stubbs L. L. 1972; Broad bean wilt virus. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses no 81:4 pp
    [Google Scholar]
  15. Wu G.-J., Bruening G. 1971; Two proteins from cowpea mosaic virus. Virology 46:596–612
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-39-1-171
Loading
/content/journal/jgv/10.1099/0022-1317-39-1-171
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error