1887

Abstract

SUMMARY

Exhaustion type hybridization was used to measure the amount of nuclear virus RNA complementary to the early (E) and late (L) polyoma virus DNA strands. At 36 h after infection between 2.5 and 7.3% of the newly synthesized virus RNA was complementary to the E-strand (-strand) and between 92.7 and 97.5% was complementary to the L-strand (+ strand). This proportion was independent of the labelling time, indicating similar accumulation of the E- and L-RNA transcripts in the nucleus. The nuclear E- and L-RNA transcripts sedimented in a similar manner through sucrose gradients.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-39-2-357
1978-05-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/39/2/JV0390020357.html?itemId=/content/journal/jgv/10.1099/0022-1317-39-2-357&mimeType=html&fmt=ahah

References

  1. Acheson H. N., Buetti E., Scherrer K., Weil R. 1971; Transcription of the polyoma virus genome: synthesis and cleavage of giant late polyoma-specific RNA. Proceedings of the National Academy of Sciences of the United States of America 68:2231–2235
    [Google Scholar]
  2. Aloni Y., Attardi G. 1971; Symmetrical in vivo transcription of mitochondrial DNA in HeLa cells. Proceedings of the National Academy of Sciences of the United States of America 68:1757–1761
    [Google Scholar]
  3. Alonl Y. 1972; Extensive symmetrical transcription of simian virus 40 DNA in virus-yielding cells. Proceedings of the National Academy of Sciences of the United States of America 69:2404–2409
    [Google Scholar]
  4. Alonl Y. 1973; Poly A and symmetrical transcription of SV40 DNA. Nature New Biology 243:2–6
    [Google Scholar]
  5. Alonl Y. 1974; Biogenesis and characterization of SV40 and polyoma RNAs in productively infected cells. Cold Spring Harbor Symposium on Quantitative Biology 39:165–178
    [Google Scholar]
  6. Alonl Y., Locker H. 1973; Symmetrical in vivo transcription of polyoma DNA and the separation of self-complementary viral and cell RNA. Virology 54:495–505
    [Google Scholar]
  7. Ben-Zeev A., Becker Y. 1975; Symmetrical transcription of herpes simplex virus DNA in infected BSC-I cells. Nature, London 254:719–722
    [Google Scholar]
  8. Fogel M., Sachs L. 1969; The activation of virus synthesis in polyoma-transformed cells. Virology 37:327–334
    [Google Scholar]
  9. Kamen R., Lindstrom D. M., Shure H., Old R. W. 1974; Virus specific RNA in cells productively infected or transformed by polyoma virus. Cold Spring Harbour Symposium on Quantitative Biology 39:187–198
    [Google Scholar]
  10. Kozak M., Roizman B. 1975; RNA synthesis in cells infected with herpes simplex virus. IX. Evidence for accumulation of abundant symmetric transcripts in nuclei. Journal of Virology 15:36–40
    [Google Scholar]
  11. Laub O., Alonl Y. 1975; Transcription of simian virus 40. V. Regulation of simian virus 40 gene expression. Journal of Virology 16:1171–1183
    [Google Scholar]
  12. Petterson W., Philipson L. 1974; Synthesis of complementary RNA sequences during productive adenovirus infection. Proceedings of the National Academy of the Sciences of the United States of America 71:4887–4891
    [Google Scholar]
  13. Winocour E. 1963; Purification of polyoma virus. Virology 19:158–168
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-39-2-357
Loading
/content/journal/jgv/10.1099/0022-1317-39-2-357
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error