1887

Abstract

SUMMARY

The induction of a type C virus from a strain of human embryonic lung cells (HEL-12) by iododeoxyuridine (IdUrd) was examined at various times during propagation. IdUrd did not elicit type C virus production immediately following initiation of cultures from frozen primary HEL-12 cells. After overnight treatment with 30 µg/ml IdUrd the cells expressed viral antigens and produced a type C virus between the 25th and 80th day of growth. Production of the induced type C virus was transient. Single-cell clones of the parental HEL-12 strain were likewise susceptible to type C virus production by IdUrd. The ability of IdUrd to induce virus terminated with the onset of spontaneous type C virus production from HEL-12 cells at between 80 and 120 days of growth.

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/content/journal/jgv/10.1099/0022-1317-42-2-399
1979-02-01
2024-05-06
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References

  1. Aaronson S. A., Stephenson J. R. 1976; Endogenous type C RNA viruses of mammalian cells. Biochimica et Biophysica Acta 458:323–354
    [Google Scholar]
  2. Aaronson S. A., Todaro G. J., Scolnick E. M. 1971; Induction of murine C-Type virus from clonal lines of virus-free BALB/3T3 cells. Science 174:157–159
    [Google Scholar]
  3. August J. T., Bolognesj D. P., Fleissner E., Gilden R. V., Nowinski R. C. 1974; A proposed nomenclature for the virion proteins of oncogenic RNA viruses. Virology 60:595–601
    [Google Scholar]
  4. Lowy D. R., Rowe W. P., Teich N. M., Hartley J. W. 1971; Murine leukemia virus: high frequency activation in vitro by 5-iododeoxyuridine. Science 174:155–156
    [Google Scholar]
  5. Lowy D. R., Chattopadhyay S. R., Teich N. M., Rowe W. P., Levine A. R. 1974; AKR murine leukemia virus genome: frequency of sequences in DNA of high-low and non-virus yielding mouse strains. Proceedings of the National Academy of Sciences of the United States of America 71:3555–3559
    [Google Scholar]
  6. McAllister R. M., Nicolson M. O., Gardner M. B., Rongey R. W., Rasheed S., Sarma P. S., Huebner R. J., Hatanaka M., Oroszlan S., Gilden R. V., Kabigting A., Vernon L. 1972; C-type virus released from cultured human rhabdomyosarcoma cells. Nature New Biology 235:3–6
    [Google Scholar]
  7. Panem S. 1976; Spontaneous type C virus expression in human embryonic lung cells (HEL-12): comparison of cloned and mixed cell populations. In Animal Virology pp 409–417 Edited by Baltimore D., Huang A., Fox C. F. New York: Academic Press;
    [Google Scholar]
  8. Panem S., Prochownik E. V., Reale F. R., Kirsten W. H. 1975; Isolation of type C virions from a normal human fibroblast strain. Science 189:297–298
    [Google Scholar]
  9. Panem S., Prochownik E. V., Knish W. H., Kirsten W. H. 1977; Cell generation and type C virus expression in the human embryonic cell strain HEL-12. Journal of General Virology 35:487–495
    [Google Scholar]
  10. Prochownik E. V., Kirsten W. H. 1977; Nucleic acid sequences of primate type C viruses in normal and neoplastic human tissues. Nature, London 267:175–177
    [Google Scholar]
  11. Prochownik E. V., Panem S., Kirsten W. H. 1976; Induction of type C virions from normal rat kidney cells by 2-deoxy-D-glucose. Journal of Virology 17:219–226
    [Google Scholar]
  12. Rowe W. P., Hartley J. W., Lander M. R., Pugh W. E., Teich N. 1971; Noninfectious AKR mouse embryo cell lines in which each cell has the capacity to be activated to produce infectious murine leukemia virus. Virology 46:866–876
    [Google Scholar]
  13. Theilen G. H., Gould D., Fowler M., Dungworth D. L. 1971; C-type virus in tumor tissue of a woolly monkey (Lagothrix spp.) with fibrosarcoma. Journal of the National Cancer Institute 47:881–889
    [Google Scholar]
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