1887

Abstract

SUMMARY

The tryptic peptides of aminoethylated coat protein from cowpea chlorotic mottle virus were isolated and their amino acid compositions determined. Twenty-three major peptides accounted for 177 of the 182 amino acid residues which comprise the coat protein. The tryptic peptides were identified with respect to their position on two-dimensional peptide maps of cowpea chlorotic mottle virus protein, to aid future analysis of coat protein variants.

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/content/journal/jgv/10.1099/0022-1317-43-1-143
1979-04-01
2024-04-28
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References

  1. Bancroft J. B., Hiebert E., Rees M. W., Markham R. 1968; Properties of cowpea chlorotic mottle virus, its protein and nucleic acid. Virology 34:224–239
    [Google Scholar]
  2. Bancroft J. B., Mclean G. D., Rees M. W., Short M. N. 1971; The effect of an arginyl to a cysteinyl replacement on the uncoating behaviour of a spherical plant virus. Virology 45:707–715
    [Google Scholar]
  3. Bancroft J. B., Ress M. W., Dawson J. R. O., Mclean G. D., Short M. N. 1972; Some properties of a temperature-sensitive mutant of cowpea chlorotic mottle virus. Journal of General Virology 16:69–81
    [Google Scholar]
  4. Bancroft J. B., Rees M. W., Johnson M. W., Dawson J. R. O. 1973; A salt-stable mutant of cowpea chlorotic mottle virus. Journal of General Virology 21:507–513
    [Google Scholar]
  5. Bancroft J. B., McDonald J. G., Rees M. W. 1976; A mutant of cowpea chlorotic mottle virus with a perturbed assembly mechanism. Virology 75:293–305
    [Google Scholar]
  6. Dawson R. M. C., Elliot W. H., Elliot D. C., Jones K. M. 1959 In Data for Biochemical Research London and New York: Oxford University Press;
    [Google Scholar]
  7. Groskopf W. R., Holleman J. W., Margoliash E., Klotz I. M. 1966; Amino acid sequences of peptides from the tryptic digest of Goldfingia gouldii hemerythrin. Biochemistry 5:3783–3796
    [Google Scholar]
  8. Guest J. R., Carlton B. C., Yanofsky C. 1967; The amino acid sequence of the A protein (alpha subunit) of the tryptophan synthetase of Escherichia coli. 1. Tryptic peptides. Journal of Biological Chemistry 242:5397–5412
    [Google Scholar]
  9. Hayashi A., Stamatoyannopoulos G., Yoshida A., Adamson J. 1971; Haemoglobin Rainier: β 145 (HC2) Tyr → CysH and haemoglobin Bethesda /S145 (HC2) Tyr → His. Nature New Biology 230:264–267
    [Google Scholar]
  10. Irreverre F. 1965; A modified Sakaguchi spray. Biochimica et Biophysica Acta in:551–552
    [Google Scholar]
  11. Jepson J. B., Smith I. 1953; Multiple dipping procedures in paper chromatography. Nature, London 172:1100–1101
    [Google Scholar]
  12. Kimmel J. R., Kato G. K., Paiva A. C. M., Smith E. L. 1962; Tryptic digest of papain. I. Isolation and sequence of some peptides from the oxidised protein. Journal of Biological Chemistry 237:2525–2534
    [Google Scholar]
  13. Offord R. E. 1966; Electrophoretic mobilities of peptides on paper and their use in the determination of amide groups. Nature, London 211:591–593
    [Google Scholar]
  14. Raftery M. A., Cole R. D. 1966; On the aminoethylation of proteins. Journal of Biological Chemistry 241:3457–3461
    [Google Scholar]
  15. Rees M. W., Short M. N., Kassanis B. 1970; The amino acid composition, antigenicity, and other characteristics of the satellite viruses of tobacco necrosis virus. Virology 40:448–461
    [Google Scholar]
  16. Reindel F., Hoppé W. 1954; A colour method for staining amino acids, peptides and proteins on paper-chromatograms and paper electrophoretograms. Chemische Berichte 87:1103–1107
    [Google Scholar]
  17. Smith I. 1953; Colour reactions on paper chromatograms by a dipping technique. Nature, London 171:43–44
    [Google Scholar]
  18. Spackman D. H., Stein W. H., Moore S. 1958; Automatic recording apparatus for use in the chromatography of amino acids. Analytical Chemistry 30:1190–1206
    [Google Scholar]
  19. Taniuchi H., Anfinsen C. B. 1966; The amino acid sequence of an extracellular nuclease of Staphylococcus aureus. I. Linear order of the fragments produced by cleavage with cyanogen bromide. Journal of Biological Chemistry 241:4366–4385
    [Google Scholar]
  20. Taniuchi H., Anfinsen C. B., Sodja A. 1967; The amino acid sequence of an extracellular nuclease of Staphylococcus aureus. II. The amino acid sequences of tryptic and chymotryptic peptides. Journal of Biological Chemistry 242:4736–4751
    [Google Scholar]
  21. Tremaine J. H., Agrawal H. O., Chidlow J. 1972; Partial sequence of the N-terminal portion of the protein of cowpea chlorotic mottle virus. Virology 48:245–254
    [Google Scholar]
  22. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulphate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  23. Yamada S., Itano H. A. 1966; Phenanthrenequinone as an analytical reagent for arginine and other monosubstituted guanidines. Biochimica et Biophysica Acta 130:538–540
    [Google Scholar]
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