1887

Abstract

SUMMARY

MDCK cells that normally would have been killed by standard lymphocytic choriomeningitis (LCM) virus were saved either by pre- or co-infection with defective interfering (DI) virus. The ability of these spared cells to produce virus-specific antigen (as well as infectious virus) and resist being killed by standard virus challenge was followed for at least 35 days. During this period both types of cultures displayed unique cycling patterns for the above characteristics. The most striking difference was the longevity of the infections. Cultures exposed to DI particles prior to standard virus became persistently infected, while co-infection with both virus types led to spontaneous curing with no trace of the previous infection. The basis for these dissimilar outcomes was traced to a hitherto undetected non-defective LCM virus (called SP) in the DI virus stocks used to preinfect MDCK cells. SP virus was not present in standard virus stocks but arose in long-term persistently infected L cells that had been initially infected with standard virus. Cloned SP virus shared species-specific antigens with standard virus, was resistant to inhibition by DI virus and was capable of turning self-curing cultures into cultures persistently synthesizing both DI and SP virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-44-1-113
1979-07-01
2024-05-01
Loading full text...

Full text loading...

/deliver/fulltext/jgv/44/1/JV0440010113.html?itemId=/content/journal/jgv/10.1099/0022-1317-44-1-113&mimeType=html&fmt=ahah

References

  1. Buchmeier M. J., Oldstone M. B. A. 1978; Virus-induced immune complex disease: identification of specific viral antigens and antibodies deposited in complexes during chronic lymphocytic choriomeningitis virus infection. Journal of Immunology 120:1297–1304
    [Google Scholar]
  2. Dutko F. J., Pfau C. J. 1978; Arenavirus defective interfering particles mask the cell-killing potential of standard virus. Journal of General Virology 38:195–208
    [Google Scholar]
  3. Dutko F. J., Wright E. A., Pfau C. J. 1976; The RNAs of defective interfering Pichinde virus. Journal of General Virology 31:417–427
    [Google Scholar]
  4. Gschwender H. H., Rutter G., Popescu M. 1975; Use of iodinated organic compounds for the density gradient centrifugation of viruses. Archives of Virology 49:359–364
    [Google Scholar]
  5. Hotchin J., Sikora E. 1973; Low-pathogenicity variant of lymphocytic choriomeningitis virus. Infection and Immunity 7:825–826
    [Google Scholar]
  6. Huang A. S., Baltimore D. 1977; Defective interfering animal viruses. In Comprehensive Virology vol 10 pp 73–116 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  7. Jahrling P. B., Hesse R. A., Metzger J. F. 1978; Radioimmunoassay for quantitation of antibodies to alphaviruses with Staphylococcal protein A. Journal of Clinical Microbiology 8:54–60
    [Google Scholar]
  8. Pfau C. J., Bergold G. H., Casals J., Johnson K. M., Murphy F. A., Pedersen I. R., Rawls W. E., Rowe W. P., Webb P. A., Weissenbacher M. C. 1974; Arenaviruses. Intervirology 4:207–213
    [Google Scholar]
  9. Pfau C. J. 1977; The role of defective interfering (DI) virus in arenavirus infections. Medicina, Buenos Aires 37:32–38
    [Google Scholar]
  10. Pfau C. J. 1978; The immunological basis of persistent infection and disease in lymphocytic choriomeningitis virus-infected mice. In Comparative and Developmental Aspects of Immunity and Disease pp 298–307 Edited by Gershwin M. E., Cooper E. L. Oxford: Pergamon Press;
    [Google Scholar]
  11. Popescu M., Lehmann-Grube F. 1976; Diversity of lymphocytic choriomeningitis virus: variation due to replication of the virus in the mouse. Journal of General Virology 30:113–122
    [Google Scholar]
  12. Popescu M., LehmannGrube F. 1977; Defective interfering particles in mice infected with lymphocytic choriomeningitis virus. Virology 77:78–83
    [Google Scholar]
  13. Pulkkinen A. J., Pfau C. J. 1970; Plaque size heterogeneity: a genetic trait of lymphocytic choriomeningitis virus. Applied Microbiology 20:123–128
    [Google Scholar]
  14. Rice R. H., Means G. E. 1971; Radioactive labeling of proteins in vitro. Journal of Biological Chemistry 246:831–832
    [Google Scholar]
  15. Staneck L. D., Welsh R. M., Trowbridge R. S., Wright E. A., Pfau C. J. 1972; Arenaviruses: cellular response to long term in vitro infection with Parana and LCM viruses. Infection and Immunity 6:444–450
    [Google Scholar]
  16. Welsh R. M., Pfau C. J. 1972; Determinants of lymphocytic choriomeningitis interference. Journal of General Virology 14:177–187
    [Google Scholar]
  17. Welsh R. M., O’Connell C. M., Pfau C. J. 1972; Properties of defective lymphocytic choriomeningitis virus. Journal of General Virology 17:355–359
    [Google Scholar]
  18. Welsh R. M., Oldstone M. B. A. 1977; Inhibition of immunologic injury of cultured cells infected with lymphocytic choriomeningitis virus: role of defective interfering virus in regulating viral antigenic expression. Journal of Experimental Medicine 145:1449–1468
    [Google Scholar]
  19. Welsh R. M., Lampert P. W., Oldstone M. B. A. 1977; Prevention of virus-induced cerebellar disease by defective-interfering lymphocytic choriomeningitis virus. Journal of Infectious Diseases 136:391–399
    [Google Scholar]
  20. Youngner J. S. 1977; Role of temperature-sensitive mutants in persistent infection. In Microbiology - 1977 pp 451–455 Edited by Schessinger D. Washington D.C.: American Society for Microbiology;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-44-1-113
Loading
/content/journal/jgv/10.1099/0022-1317-44-1-113
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error