1887

Abstract

SUMMARY

In Vero cells infected with Bunyamwera virus there is a rapid inhibition of cell RNA and protein synthesis to levels of 30 and 3% respectively of the control rate, both the rate of inhibition and the time lag before its initiation being multiplicity dependent. Using u.v.-irradiated virus, investigation of the mechanism of inhibition of host cell protein synthesis indicates that synthesis of new virus components is required for inhibition to occur. Quantitative comparison of the proteins synthesized in infected cells shows that at higher m.o.i. synthesis of virus, as well as cellular proteins, is inhibited. Bunyamwera virus-infected Vero cells synthesized three virus-specific proteins identified as the structural virion proteins. Nucleoprotein is synthesized predominantly early in infection while the major envelope glycoprotein and the minor glycoprotein are synthesized predominantly late in the infection cycle.

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1979-07-01
2024-05-01
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References

  1. Baltimore D. 1969; The replication of picornaviruses. In The Biochemistry of Viruses pp 101–176 Edited by Levy H. B. New York and London: Marcel Dekker;
    [Google Scholar]
  2. Bergold C. H., Mazzali R. 1968; Plaque formation by arboviruses. Journal of General Virology 2:273–284
    [Google Scholar]
  3. Bouloy M., Krams-Ozden S., Horodniceanu F., Hannoun C. 1973/4; Three-segment RNA genome of Lumbo virus (Bunyavirus). Intervirology 2:173–180
    [Google Scholar]
  4. Clarke D. H., Casals J. 1958; Techniques for haemagglutination and haemagglutination-inhibition with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  5. Gentsch-Bishop D. H. L., Obijeski J. F. 1977; The virus particle nucleic acids and proteins of four bunyaviruses. Journal of General Virology 34:257–268
    [Google Scholar]
  6. Huang A. S., Wagner R. R. 1965; Inhibition of cellular RNA synthesis by nonreplicating vesicular stomatitis virus. Proceedings of the National Academy of Sciences of the United States of America 54:1579–1584
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  8. Maizel J. V. Jun 1969; Acrylamide gel electrophoresis of proteins and nucleic acids. In Fundamental Techniques in Virology pp 334–362 Edited by Habel K., Salzman N. P. London and New York: Academic Press;
    [Google Scholar]
  9. Mans R. J., Novelli G. D. 1961; Measurement of the incorporation of radioactive amino acids into protein by a filter-paper disk method. Archives of Biochemistry and Biophysics 94:48–53
    [Google Scholar]
  10. Martin E. M., Kerr I. M. 1968; Virus-induced changes in host cell macromolecular synthesis. In The Molecular Biology of Viruses pp 15–46 Edited by Crawford L. V., Stoker M. G. P. Symposium of the Journal of General Virology No 18 London: Cambridge University Press;
    [Google Scholar]
  11. Metz D. H. 1975; Discrimination between viral and cellular macromolecular synthesis. In Control Processes in Virus Multiplication pp 323–353 Edited by Burke D. C., Russell W. C. Symposium of the Journal of General Virology No 25 London: Cambridge University Press;
    [Google Scholar]
  12. Moss B. 1968; Inhibition of HeLa cell protein synthesis by the vaccinia virion. Journal of Virology 2:1028–1037
    [Google Scholar]
  13. Obijeski J. F., Bishop D. H. L., Murphy F. A., Palmer E. L. 1976a; Structural proteins of La Crosse virus. Journal of Virology 19:985–997
    [Google Scholar]
  14. Obijeski J. F., Bishop D. H. L., Palmer E. L., Murphy F. A. 1976b; Segmented genome and nucleocapsid of La Crosse virus. Journal of Virology 20:664–675
    [Google Scholar]
  15. Obijeski J. F., Murphy F. A. 1977; Bunyaviridae: Recent biochemical developments. Journal of General Virology 37:1–14
    [Google Scholar]
  16. Penman S., Summers D. 1965; Effects of host cell metabolism following synchronous infection with poliovirus. Virology 27:614–620
    [Google Scholar]
  17. Pennington T. H., Pringle C. R., Mcrae M. A. 1977; Bunyamwera virus-induced polypeptide synthesis. Journal of Virology 24:397–400
    [Google Scholar]
  18. Pettersson R. F. 1974; Effect of Uukuniemi virus infection on host cell macromolecule synthesis. Medical Biology 52:90–97
    [Google Scholar]
  19. Pettersson R. F., Von Bonsdorff C. H. 1975; Ribonucleoproteins of Uukuniemi virus are circular. Journal of Virology 15:383–392
    [Google Scholar]
  20. Rose J. K., Trachsel H., Leong K., Baltimore D. 1978; Inhibition of translation by poliovirus: Inactivation of a specific initiation factor. Proceedings of the National Academy of Sciences of the United States of America 75:2732–2736
    [Google Scholar]
  21. Samso A., Bouloy M., Hannoun C. 1975; Presence of circular ribonucleoproteins in Lumbo virus (Bunyavirus). Comptes Rendus hebdomadaires des seances de l’Academie des Sciences de Paris Series D 280:779–782
    [Google Scholar]
  22. Shapiro D., Kos K. A., Russell P. K. 1973; Protein synthesis in Japanese encephalitis virus-infected cells. Virology 56:95–109
    [Google Scholar]
  23. Strauss J. H. Jun, Burge B. W., Darnell J. E. 1969; Sindbis virus infection of chick and hamster cells: synthesis of virus-specific proteins. Virology 37:367–376
    [Google Scholar]
  24. Trent D. W., Qureshi A. A. 1971; Structural and nonstructural proteins of Saint Louis encephalitis virus. Journal of Virology 7:379–388
    [Google Scholar]
  25. Von Bonsdorff C. H., Pettersson R. F. 1975; Surface structure of Uukuniemi virus. Journal of Virology 16:1296–1307
    [Google Scholar]
  26. Wertz G. W., Youngner J. S. 1972; Inhibition of protein synthesis in L cells infected with vesicular stomatitis virus. Journal of Virology 9:85–89
    [Google Scholar]
  27. Westaway E. G. 1973; Proteins specified by group B togaviruses in mammalian cells during productive infections. Virology 51:454–465
    [Google Scholar]
  28. Yamazaki S., Wagner R. R. 1970; Action of interferon: kinetics and differential effects on viral functions. Journal of Virology 6:421–429
    [Google Scholar]
  29. Zweerink H. J., Mcdowell M. J., Joklik W. K. 1971; Essential and nonessential non-capsid reovirus proteins. Virology 45:716–723
    [Google Scholar]
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