1887

Abstract

SUMMARY

KB cells were infected with H536 or H5125, two adenovirus type 5 (Ad5) mutants with a temperature-sensitive synthesis of virus DNA. Infection was started at the nonpermissive temperature and at 16 h p.i. the temperature was shifted down to the permissive temperature. Shortly after the shift-down H5125-infected cells showed an accumulation of purely single-stranded DNA of virus origin, which was not observed in H536-infected cells. This single-stranded DNA has been characterized by hybridization and sedimentation analysis. It was found that the single-stranded DNA was derived from both complementary strands and consisted of short fragments. The observation that the single-stranded DNA accumulates in H5125-infected cells under conditions in which the amount of DNA binding protein is reduced, suggests that the DNA-binding protein is not only involved in initiation, but also in elongation of nascent strands.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-44-1-25
1979-07-01
2024-05-01
Loading full text...

Full text loading...

/deliver/fulltext/jgv/44/1/JV0440010025.html?itemId=/content/journal/jgv/10.1099/0022-1317-44-1-25&mimeType=html&fmt=ahah

References

  1. Ariga H., Shimojo H. 1977; Initiation and termination sites of adenovirus 12 DNA replication. Virology 78:415–424
    [Google Scholar]
  2. Ensinger M. J., Ginsberg H. S. 1971; Selection and preliminary characterization of temperature-sensitive mutants of type 5 adenovirus. Journal of Virology 10:328–339
    [Google Scholar]
  3. Flint S. J., Berget S. M., Sharp P. A. 1976; Characterization of single-stranded viral DNA sequences present during replication of adenovirus types 2 and 5. Cell 9:559–571
    [Google Scholar]
  4. Ginsberg H. S., Ensinger M. J., Kauffman R. S., Mayer A. J., Lundholm U. 1974; Cell transformation: a study of regulation with type 5 and 12 adenovirus temperature-sensitive mutants. Cold Spring Harbor Symposia on Quantitative Biology 39:419–426
    [Google Scholar]
  5. Ginsberg H. S., Lundholm U., Linne T. 1977; Adenovirus DNA binding protein in cells infected with wild type 5 adenovirus and two DNA-minus temperature-sensitive mutants H5ts125 and H5ts49. Journal of Virology 23:142–151
    [Google Scholar]
  6. Kathmann P., Schick J., Winnacker E. L., Doerfler W. 1976; Isolation and characterization of temperature-sensitive mutants of adenovirus type 2. Journal of Virology 19:45–53
    [Google Scholar]
  7. Levine A. J., van der Vliet P. C., Sussenbach J. S. 1976; The replication of papova virus and adenovirus DNA. Current Topics in Microbiology and Immunology 73:67–124
    [Google Scholar]
  8. Linne T., Jornvall H., Philipson L. 1977; Purification and characterization of the phosphorylated DNA-binding protein from adenovirus-type 2-infected cells. European Journal of Biochemistry 76:481–490
    [Google Scholar]
  9. Rice R. H., Means G. E. 1971; Radioactive labeling of proteins in vitro. Journal of Biological Chemistry 246:831–832
    [Google Scholar]
  10. Sambrook J., Williams J. F., Sharp P. A., Grodzicker T. 1975; Physical mapping of temperature-sensitive mutations of adenoviruses. Journal of Molecular Biology 97:369–390
    [Google Scholar]
  11. Shiroki K., Shimojo H. 1974; Analysis of adenovirus 12 temperature-sensitive mutants defective in viral DNA replication. Virology 61:474–485
    [Google Scholar]
  12. Sugawara K., Gilead A., Green M. 1977; Purification and molecular characterization of adenovirus type 2 DNA binding protein. Journal of Virology 21:338–346
    [Google Scholar]
  13. Sussenbach J. S., Kuijk M. G. 1977; Studies on the mechanism of replication of adenovirus DNA. V. The location of termini of replication. Virology 77:149–157
    [Google Scholar]
  14. Sussenbach J. S., Kuijk M. O. 1978; Studies on the mechanism of adenovirus DNA replication. IV. Localization of the origins of the displacement synthesis. Virology 84:509–517
    [Google Scholar]
  15. Sussenbach J. S., van der Vliet P. C. 1972; Viral DNA synthesis in isolated nuclei from adenovirus infected KB cells. FEBS letters 21:7–10
    [Google Scholar]
  16. Sussenbach J. S., Ellens D. J., Jansz H. S. 1973; Studies on the mechanism of adenovirus DNA replication. II. The nature of the single-stranded DNA in replicative intermediates. Journal of Virology 12:1131–1138
    [Google Scholar]
  17. Sussenbach J. S., Tolun A., Pettersson U. 1976; The nature of the single-stranded DNA in replicating adenovirus type 5 DNA. Journal of Virology 20:532–534
    [Google Scholar]
  18. Tolun A., Pettersson U. 1975; Terminations sites for adenovirus type 2 DNA replication. Journal of Virology 16:759–766
    [Google Scholar]
  19. van der Vliet P. C., Levine A. J. 1973; DNA binding proteins specific for cells infected with adenovirus. Nature, London 246:170–174
    [Google Scholar]
  20. van der Vliet P. C., Sussenbach J. S. 1972; The mechanism of adenovirus DNA synthesis in isolated nuclei. European Journal of Biochemistry 30:584–592
    [Google Scholar]
  21. van der Vliet P. C., Sussenbach J. S. 1975; An adenovirus type 5 gene function required for initiation of viral DNA replication. Virology 67:415–426
    [Google Scholar]
  22. van der Vliet P. C., Levine A. J., Ensinger M. J., Ginsberg H. S. 1975; Thermolabile DNA binding proteins from cells infected with a temperature-sensitive mutant of adenovirus defective in viral DNA synthesis. Journal of Virology 15:348–354
    [Google Scholar]
  23. van der Vliet P. C., Zandberg J., Jansz H. S. 1977; Evidence for a function of the adenovirus DNA binding protein in initiation of DNA synthesis as well as in elongation of nascent DNA chains. Virology 80:98–110
    [Google Scholar]
  24. Wilkie N. M., Ustacelebi C., Williams J. F. 1973; Characterization of temperature-sensitive mutants of adenovirus type 5: nucleic acid synthesis. Virology 51:499–503
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-44-1-25
Loading
/content/journal/jgv/10.1099/0022-1317-44-1-25
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error