1887

Abstract

SUMMARY

A comparative study of and phosphorylation of murine mammary tumour virus, a type B RNA virus, is reported. The protein kinase activity associated with murine mammary tumour virus catalysed the phosphorylation of endogenous virus polypeptides. This kinase activity required a divalent metal cation, a non-ionic detergent, and was stimulated in the presence of dithiothreitol. Exogenous cyclic AMP was not required. The P-labelled products of the reaction were completely sensitive to pronase digestion and the phosphate was attached mainly by phosphomonoester linkage to serine residues. As determined by SDS-polyacrylamide gel electrophoresis, heterogeneous labelling of major and minor virus polypeptides was observed under conditions.

In contrast, the labelling of type B virus produced by a continuous cell line (MuMT-73), established from pooled mammary adenocarcinomas of Balb/cfC3H mice, demonstrated specific phosphoproteins associated with murine mammary tumour virus. The major phosphorylated proteins were found to have mol. wt. of 18000 and 12000 (p18 and p12) after isolation by molecular sieving chromatography and analysis by gel electrophoresis.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-44-3-669
1979-09-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/44/3/JV0440030669.html?itemId=/content/journal/jgv/10.1099/0022-1317-44-3-669&mimeType=html&fmt=ahah

References

  1. August J. T., Bolognesi D. P., Fleissner E., Gilden R. V., Nowinski R. C. 1974; A proposed nomenclature for the virion proteins of oncogenic RNA viruses. Virology 60:595–601
    [Google Scholar]
  2. Collett M. S., Erikson R. L. 1978; Protein kinase activity associated with the avian sarcoma virus src gene product. Proceedings of the National Academy of Sciences of the United States of America 75:2021–2024
    [Google Scholar]
  3. Dion A. S., Moore D. H. 1973; Cation requirements for RNA- and DNA-templated DNA polymerase activities of B-type oncogenic RNA viruses (MuMTV). In Polyamines in Normal and Neoplastic Growth pp 323–334 Edited by Russell D. H. New York: Raven Press;
    [Google Scholar]
  4. Dion A. S., Moore D. H. 1977; Some biochemical aspects of murine mammary tumor virus and the putative human mammary tumor virus. In Recent Advances in Cancer Research: Cell Biology, Molecular Biology, and Tumor Virology vol II pp 69–87 Edited by Gallo R. C. Cleveland, Ohio: CRC Press, Inc;
    [Google Scholar]
  5. Dion A. S., Williams C. J., Pomenti A. A. 1977; The major structural proteins of murine mammary tumor virus: techniques for isolation. Analytical Biochemistry 82:18–28
    [Google Scholar]
  6. Enami J., Nandi S. 1977; A sensitive radioimmunoassay for a component of mouse casein. Journal of Immunological Methods 18:235–244
    [Google Scholar]
  7. Greenwood F. C., Hunter W. M., Glover J. S. 1963; The preparation of I-131-labeled human growth hormone of high specific radioactivity. Biochemical Journal 89:114–123
    [Google Scholar]
  8. Hatanaka M., Twiddy E., Gilden R. V. 1972; Protein kinase associated with RNA tumor viruses and other budding RNA viruses. Virology 47:536–538
    [Google Scholar]
  9. Lai M. M. C. 1976; Phosphoproteins of Rous sarcoma viruses. Virology 74:287–301
    [Google Scholar]
  10. Moyer S. A., Summers D. F. 1974; Phosphorylation of vesicular stomatitis virus in vivo and in vitro. Journal of Virology 13:455–465
    [Google Scholar]
  11. Pal B. K., McAllister R. M., Gardner M. B., Roy-Burman P. 1975; Comparative studies on the structural phosphoproteins of mammalian type C viruses. Journal of Virology 16:123–131
    [Google Scholar]
  12. Paoletti E., Moss B. 1972; Protein kinase and specific phosphate acceptor proteins associated with vaccinia virus cores. Journal of Virology 10:417–424
    [Google Scholar]
  13. Randall C. C., Rogers H. W., Downer D. N., Gentry G. A. 1972; Protein kinase activity in equine herpesvirus. Journal of Virology 9:216–222
    [Google Scholar]
  14. Rosemond H., Moss B. 1973; Phosphoprotein component of vaccinia virions. Journal of Virology 11:961–970
    [Google Scholar]
  15. Roux L., Kolakofsky D. 1974; Protein kinase associated with Sendai virions. Journal of Virology 13:545–547
    [Google Scholar]
  16. Russell W. C., Skehel J. J., MacHado R., Pereira H. G. 1972; Phosphorylated polypeptides in adenovirus-infected cells. Virology 50:931–934
    [Google Scholar]
  17. Sarkar N. H., Dion A. S. 1975; Polypeptides of the mouse mammary tumor virus. I. Characterization of two group-specific antigens. Virology 64:471–491
    [Google Scholar]
  18. Sarkar N. H., Pomenti A. A., Dion A. S. 1977; Replication of mouse mammary tumor virus in tissue culture. I. Establishment of a mouse mammary tumor cell line, virus characterization, and quantitation of virus. Virology 77:12–30
    [Google Scholar]
  19. Schrecker A. W., Sporn M. B., Gallo R. C. 1972; Inhibition of RNA-dependent DNA polymerase by thymidylate derivatives. Cancer Research 32:1547–1553
    [Google Scholar]
  20. Silberstein H., August J. T. 1976; Characterization of a virion protein kinase as a virus-specified enzyme. Journal of Biological Chemistry 251:3185–3190
    [Google Scholar]
  21. Strand M., August J. T. 1971; Protein kinase and phosphate acceptor proteins in Rauscher murine leukemia virus. Nature New Biology 233:137–140
    [Google Scholar]
  22. Tan K. B., Sokol R. 1972; Structural proteins of simian virus 40: phosphoproteins. Journal of Virology 10:985–994
    [Google Scholar]
  23. Waite M. R. F., Lubin M., Jones K. J., Bose H. R. 1974; Phosphorylated proteins of sindbis virus. Journal of Virology 13:244–246
    [Google Scholar]
  24. Zimmerman S. B., Sandeen G. 1966; The ribonuclease activity of crystallized pancreatic deoxyribonuclease. Analytical Biochemistry 14:269–277
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-44-3-669
Loading
/content/journal/jgv/10.1099/0022-1317-44-3-669
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error