1887

Abstract

SUMMARY

In street rabies-infected mice, expression of delayed type hypersensitivity (DTH) measured by the footpad test was revealed by challenge with inactivated fixed rabies virus (RV). The use of BCG as an adjuvant of cell-mediated immunity (CMI) was necessary for the production of significant DTH levels. Typical DTH kinetics were obtained, with a maximum at 24 h after the challenge. DTH was also found to be at highest levels 4 days after infection with street rabies virus. DTH could also be revealed with street rabies virus in RV immunized mice. Adoptive transfer of lymphoid cells from a street rabies infected donor to normal recipient mice was performed and DTH was tested with RV. Susceptibility of DTH to immunosuppression by cyclophosphamide treatment was also assayed in street rabies virus-infected mice and in adoptively-sensitized recipient mice. These results and the relationship between DTH and CMI in rabies infection and immunization are discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-47-1-183
1980-03-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/47/1/JV0470010183.html?itemId=/content/journal/jgv/10.1099/0022-1317-47-1-183&mimeType=html&fmt=ahah

References

  1. Atanasiu P., Nozaki J., Savy V., Eyquem A. 1977a; Evaluation de l’immunité cellulaire après vaccination rabique chez l’homme. Comptes Rendus hebdomadaires des Seances de l’ Académie des Sciences, Paris 285D:1187–1190
    [Google Scholar]
  2. Atanasiu P., Tsiang H., Lavergne M., Chermann J. -C. 1977b; Purification par centrifugation zonale d’un vaccin antirabique humain obtenu sur cellules rénales de foetus bovin. Annales de Microbiologie (Institut Pasteur) 128B:297–302
    [Google Scholar]
  3. Blancou J., Andral L., Lagrange P. H., Tsiang H. 1979; Kinetics of different specific immunological parameters after rabies vaccination in mice. Infection and Immunity 24:600–605
    [Google Scholar]
  4. Härfast B., Anderson T., Grandien M. 1977; Enhanced cytotoxicity of human lymphocytes against rabies infected cells by rabies specific antibodies. Scandinavian Journal of Immunology 6:1107–1112
    [Google Scholar]
  5. Kaplan M. M., Wiktor T. J., Koprowski H. 1975; Pathogenesis of rabies in immunodeficient mice. Journal of Immunology 114:1761–1765
    [Google Scholar]
  6. Kerckhaert J. A., Berg G. J. V. D., Hofhuis F. M. A. 1974; Influence of cyclophosphamide on the delayed hypersensitivity in the mouse after immunization with histocompatibility antigen. Journal of Immunology 113:1801–1806
    [Google Scholar]
  7. Lagrange P. H., Mackaness G. B., Miller T. E. 1974; Potentiation of T cell mediated immunity by selective suppression of antibody formation with cyclophosphamide. Journal of Experimental Medicine 139:1529–1539
    [Google Scholar]
  8. Lagrange P. H., Tsiang H., Hurtrel B., Ravisse P. 1978; Delayed type hypersensitivity to rabies virus in mice: assay of active or passive sensitization by the footpad test. Infection and Immunity 21:931–939
    [Google Scholar]
  9. Mackaness G. B. 1971; Delayed type hypersensitivity on the mechanism of cellular resistance to infection. In Progress in Immunology pp 413–423 Edited by Amos B. New York: Academic Press;
    [Google Scholar]
  10. Mackaness G. B., Lagrange P. H., Miller T. E., Ishibashi I. 1974; The formation of activated T cells. In Activation of Macrophages pp 193–208 Edited by Wagner W. H., Hahn H. Amsterdam: Excerpta Medica;
    [Google Scholar]
  11. Metaxas M. N., Metaxas-Buehler M. 1955; Studies on the cellular transfer of tuberculin sensitivity in the guinea pig. Journal of Immunology 75:333–347
    [Google Scholar]
  12. Miller T. E., Mackaness G. B., Lagrange P. H. 1973; Immunopotentiation with BCG: II. Modulation of the response to sheep red blood cells. Journal of the National Cancer Institute 51:1669–1675
    [Google Scholar]
  13. Miller A., Morse H. C. III, Winkelstein J., Nathanson N. 1978; The role of antibody in recovery from experimental rabies. Effect of depletion of B and T cells. Journal of Immunology 121:321–326
    [Google Scholar]
  14. Nozaki J., Atanasiu P. 1976; Etude comparative in vitro des antigènes rabiques et des virus apparentés à la rage par la technique des lymphocytes sensibilisés. Annales de Microbiologie Institut Pasteur) 127A:429–438
    [Google Scholar]
  15. Peters R. L., Donahoe R. M., Kellof G. L. 1975; Assay in the mouse for delayed type hypersensitivity to murine leukemia virus. Journal of the National Cancer Institute 55:1089–1095
    [Google Scholar]
  16. Tsiang H., Atanasiu P. 1975; Effet de la cyclophosphamide sur l’infection rabique chez la souris. Protection conférée par la sérothérapie. Comptes Rendus hebdomadaires des seances de l’ Académie des Sciences (Paris) 281:957–960
    [Google Scholar]
  17. Turner G. S. 1973; Humoral and cellular immune response of mice to rabies and smallpox vaccines. Nature, London 241:90–92
    [Google Scholar]
  18. Turner G. S. 1976; Thymus dependence of rabies vaccine. Journal of General Virology 33:535–538
    [Google Scholar]
  19. Wiktor T. J., Kamo I., Koprowski H. 1974; In vitro stimulation of rabbit lymphocytes after immunization with live and inactivated rabies vaccine. Journal of Immunology 112:2013–2019
    [Google Scholar]
  20. Wiktor T. J., Doherty P. C., Koprowski H. 1977a; In vitro evidence of cell mediated immunity after exposure of mice to both live and inactivated rabies virus. Proceedings of the National Academy of Sciences of the United States of America 74:334–338
    [Google Scholar]
  21. Wiktor T. J., Doherty P. C., Koprowski H. 1977b; Suppression of cell mediated immunity by street rabies virus. Journal of Experimental Medicine 145:1617–1622
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-47-1-183
Loading
/content/journal/jgv/10.1099/0022-1317-47-1-183
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error