1887

Abstract

SUMMARY

The DNA sequence homology relationships among six lepidopteran nuclear polyhedrosis viruses (NPVs) have been explored by hybridization of P-labelled NPV DNAs to Southern blots of restriction endonuclease-digested NPV DNA. The NPV (AcMNPV) shows extensive DNA sequence homology throughout the entire genome with the NPV (RoMNPV). Both the NPV (OpMNPV) and the NPV (PdMNPV) share homologous regions, equivalent to 1% of the DNA genome, with AcMNPV and RoMNPV. This homology is localized in two regions on the AcMNPV physical map although other regions are also weakly homologous. Approx. 1% of the DNA of OpMNPV and PdMNPV show sequence homology with each other; the homology is primarily localized in two to four regions of the genomes. NPV (HzSNPV) and NPV (TnSNPV) share less than 0.2% sequence homology with the MNPVs and share less than 0.2% sequence homology with each other.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-48-1-161
1980-05-01
2024-05-07
Loading full text...

Full text loading...

/deliver/fulltext/jgv/48/1/JV0480010161.html?itemId=/content/journal/jgv/10.1099/0022-1317-48-1-161&mimeType=html&fmt=ahah

References

  1. Burgess S. 1977; Molecular weights of lepidopteran baculovirus DNAs: derivation by electron microscopy. Journal of General Virology 37:501–510
    [Google Scholar]
  2. Cibulsky R. J., Harper J. D., Gudauskas R. T. 1977; Biochemical comparison of virion proteins from five nuclear polyhedrosis viruses infecting plusiine larvae (Lepidoptera: Noctuidae). Journal of Invertebrate Pathology 30:303–313
    [Google Scholar]
  3. Harper J. D. 1976; Cross–infectivity of six nuclear polyhedrosis virus isolates to plusiine hosts. Journal of Invertebrate Pathology 27:275–277
    [Google Scholar]
  4. Jurkovicova M., Van Touw J. H., Sussenbach J. S., Ter Schegget J. 1979; Characterization of the nuclear polyhedrosis virus DNA of Adoxophyes orana and of Barathra brassicae. Virology 93:8–19
    [Google Scholar]
  5. Kelly D. C. 1977; The DNA contained by nuclear polyhedrosis viruses isolated from four Spodoptera spp (Lepidoptera: Noctuidae): genome size and homology assessed by DNA reassociation kinetics. Virology 76:468–471
    [Google Scholar]
  6. Knudson D. L., Tinsley T. W. 1978; Replication of a nuclear polyhedrosis virus in a continuous cell line of Spodoptera frugiperda: partial characterization of the viral DNA, comparative DNA–DNA hybridization and patterns of DNA synthesis. Virology 87:42–57
    [Google Scholar]
  7. Krywienczyk J., Bergold G. H. 1961; Serological studies of inclusion-body proteins by agar diffusion techniques. Journal of Insect Pathology 3:15–28
    [Google Scholar]
  8. Lee H. H., Miller L. K. 1978; Isolation of genotypic variants of Autographa californica nuclear polyhedrosis virus. Journal of Virology 27:754–767
    [Google Scholar]
  9. Lee H. H., Miller L. K. 1979; Isolation, complementation and initial characterization of temperature-sensitive mutants of the baculovirus Autographa californica nuclear polyhedrosis virus. Journal of Virology 31:240–252
    [Google Scholar]
  10. McCarthy W. J., Liu S-Y. 1976; Electrophoretic and serological characterization of Porthetria dispar polyhedron protein. Journal of Invertebrate Pathology 28:57–65
    [Google Scholar]
  11. Miller L. K., Dawes K. P. 1978a; Restriction endonuclease analysis for the identification of baculovirus pesticides. Journal of Applied and Environmental Microbiology 35:411–421
    [Google Scholar]
  12. Miller L. K., Dawes K. P. 1978b; Restriction endonuclease analysis to distinguish two closely related nuclear polyhedrosis viruses: Autographa californica MNPV and Trichoplusia ni MNPV. Journal of Applied and Environmental Microbiology 35:1206–1210
    [Google Scholar]
  13. Miller L. K., Dawes K. P. 1979; Physical map of the DNA genome of Autographa californica nuclear polyhedrosis virus. Journal of Virology 29:1044–1055
    [Google Scholar]
  14. Rohrmann G. F. 1977; Characterization of N-po!yhedrin of two baculovirus strains pathogenic for Orgyia pseudotsugata. Biochemistry 16:1631–1634
    [Google Scholar]
  15. Rohrmann G. F., Carnegie J. W., Martigoni M. E., Beaudreau C. S. 1977; Characterization of the genome of the nucleopolyhedrosis bundle virus pathogenic for Orgyia pseudotsugata. Virology 79:334–338
    [Google Scholar]
  16. Rohrmann G. F., Mcparland R. H., Martignoni M. E., Beaudreu G. S. 1978; Genetic relatedness of two nucleopolyhedrosis viruses pathogenic for Orgyia pseudotsugata. Virology 84:213–217
    [Google Scholar]
  17. Smith G. E., Summers M. D. 1978; Analysis of baculovirus genomes with restriction endonucleases. Virology 89:517–527
    [Google Scholar]
  18. Smith K. M. 1976 In Virus–Insect Relationships London: Longham;
    [Google Scholar]
  19. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  20. Summers M. D. 1977 In Beltsville Symposium in Agricultural Research. I. Virology in Agriculture pp 233–246 Edited by Romberger J. A. Montclair, N,. J.: Allanheld, Osmun and Co;
    [Google Scholar]
  21. Summers M. D., Smith G. E. 1978; Baculovirus structural polypeptides. Virology 84:390–402
    [Google Scholar]
  22. Summers M. D., Engler R., Falcon L. A., Vail P. V. (editors) 1975 In Baculoviruses for Insect Pest Control: Safety Considerations Washington D,. C.: American Society for Microbiology;
    [Google Scholar]
  23. Upholt W. B. 1977; Estimation of sequence divergence from comparison of restriction endonuclease digests. Nucleic Acids Research 41257–1265
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-48-1-161
Loading
/content/journal/jgv/10.1099/0022-1317-48-1-161
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error