1887

Abstract

SUMMARY

Results of analysing antigenic variation in the haemagglutinin (HA) molecule of naturally occurring influenza A (H1N1) viruses from 1977 to 1979 with monoclonal antibodies were found to be dependent in some instances on the test system used. In several instances A/USSR/90/77 HA-specific monoclonal antibodies had sharply reduced haemagglutination-inhibition (HI) titres with variant virus although they bound to the variant and A/USSR/90/77 HAs with similar efficiencies as judged by titration in a sensitive and accurate solid-phase immunofluorimetric assay. In another instance, the converse situation was observed: monoclonal antibodies having a reduced efficiency of binding to the HA of a variant virus nevertheless had comparable HI titres with the variant and with A/USSR/90/77. The chemical basis and epidemiological significance of these observations remain to be elucidated. Nevertheless, the finding that the reaction of monoclonal antibodies can, in some cases, be markedly dependent on the test system employed is of significance for the efficient design and correct interpretation of immunochemical studies which employ monoclonal antibodies to investigate the basis for variation in influenza strains.

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1981-06-01
2024-05-03
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References

  1. Dowdle W. R., Kendal A. P., Noble G. R. 1979; Influenza viruses. In Diagnostic Procedures for Viral, Rickettsial and Chlamydial Infections pp 585–609 Edited by Lennette E. H., Schmidt N. J. Washington, D. C.: American Public Health Association;
    [Google Scholar]
  2. Fazekas de stgroth S. 1961; Methods in immunochemistry of viruses. 2. Evaluation of parameters for equilibrium measurements. A ustralian Journal of Experimental Biology 39:563–582
    [Google Scholar]
  3. Fazekasde st. groth S., Webster R. G. 1966; Disquisitions on original antigenic sin. 1. Evidence in man. Journal of Experimental Medicine 124:331–345
    [Google Scholar]
  4. Frankel M. G., Gerhard W. 1979; The rapid determination of binding constants for antiviral antibodies by a radioimmunoassay. An analysis of the interaction between hybridoma proteins and influenza virus. Molecular Immunology 16:101–106
    [Google Scholar]
  5. Gerhard W., Yewdell J., Frankel M. 1980; An experimental approach to define the antigenic structures of the hemagglutinin molecule of A/PR/8/34. In Structure and Variation in Influenza Virus pp 273–281 Edited by Laver W. G., Air G. M. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  6. Kendal A. P., Joseph J. M., Kobayashi G., Nelson D., Reyes C. R., Ross M. R., Sarandria J. L., White R., Woodall D. F., Noble G. R., Dowdle W. R. 1979; Laboratory-based surveillance of influenza virus in the United States during the winter of 1977–1978. 1. Periods of prevalence of H1N1 and H3N2 influenza A strains, their relative rates of isolation in different age groups and detection of antigenic variants. American Journal of Epidemiology 110:449–461
    [Google Scholar]
  7. Nakajima S., Kendal A. P. 1981; Studies on antigenic variation in influenza A (H1N1) virus: significance of certain antigenic determinants on the hemagglutinin molecule. In Proceedings of the Negative Strand Virus Meeting Edited by Bishop D., Compans R. New York: Elsevier/North-Holland; (in press)
    [Google Scholar]
  8. Phillips D. J., Kendal A. P., Webster R. G., Feorino P. M., Reimer C. B. 1980a; Detection of monoclonal influenza antibodies synthesized in culture by hybridoma cells with a solid-phase indirect immuno-fluorometric assay. Journal of VirologicalMethods 1:275–283
    [Google Scholar]
  9. Phillips D. J., Reimer C. B., Wells T. W., Black C. M. 1980b; Quantitative characterization of specificity and potency of conjugated antibody with solid-phase, antigen bead standards. Journal of Immunological Methods 34:315–327
    [Google Scholar]
  10. Reimer C. B., Phillips D. J., Black C. M., Wells T. W. 1978; Standardization of ligand binding assay for alpha-fetoprotein. In Immunofluorescence and Related Staining Techniques pp 189–200 Edited by Knapp W., Holubar F., Wick G. New York: Elsevier/North-Holland Biomedical Press;
    [Google Scholar]
  11. Schild G. C. 1970; Studies with antibody to the purified haemagglutinin of an influenza Ao virus. Journal of General Virology 9:191–200
    [Google Scholar]
  12. Schulman J. L. 1975; Immunology of influenza. In The Influenza Viruses and Influenza pp 373–393 Edited by Kilbourne E. D. New York: Academic Press;
    [Google Scholar]
  13. Six H. R., Kasel J. A. 1978; Radioimmunoprecipitation assay for quantitation of serum antibody to the hemagglutinin of type A influenza virus. Journal of Clinical Microbiology 7:165–171
    [Google Scholar]
  14. van wyke K. L., Hinshaw V. S., Bean W. J., Webster R. G. 1980; Antigenic variation of influenza A virus nucleoprotein detected with monoclonal antibodies. Journal of Virology 35:24–30
    [Google Scholar]
  15. Virelizier J.-L. 1975; Host defenses against influenza virus: the role of anti-hemagglutinin antibody. Journal of Immunology 115:434–439
    [Google Scholar]
  16. Webster R. G., Laver W. G. 1980; Determination of the number of nonoverlapping antigenic areas on Hong Kong (H3N2) influenza virus hemagglutinin with monoclonal antibodies and the selection of variants with potential epidemiological significance. Virology 104:139–148
    [Google Scholar]
  17. Webster R. G., Kendal A. P., Gerhard W. 1979; Analysis of antigenic drift in recently isolated influenza A(H1N1) viruses using monoclonal antibody preparations. Virology 96:256–264
    [Google Scholar]
  18. Yewdell J. W., Webster R. G., Gerhard W. U. 1979; Antigenic variation in three distinct determinants of an influenza type A haemagglutinin molecule. Nature, London 279:246–248
    [Google Scholar]
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