1887

Abstract

SUMMARY

Three kinds of temperate phages were differentiated by serological, sedimentation and electron microscopic studies. Phage S1 had a long hexagonal head and a long tail, a density in CsSO of 1.365 g/ml and belonged to serological group A; phage S2 had a short hexagonal head and a short tail, a density of 1.385 to 1.392 g/ml and belonged to serological group B; phage S3 had a shape similar to phage S2 except for some minor differences, but had a density of 1.416 g/ml and belonged to serological group F. MS27 was found to be singly lysogenic for S2. However, MS3878 was a doubly lysogenic strain carrying S1 and S2 and E169 was a triply lysogenic strain carrying S1, S2 and S3. All these temperate phages showed similarity in shape to typing phages belonging to the same serological group. The temperate phages revealed the presence of a hexagonal baseplate with spikes. The burst sizes of phages S1, S2 and S3 were about 50, 110 and 120 respectively. The DNA from S1, S2 and S3 ranged from 29.4 to 30 megadaltons in size.

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1981-08-01
2024-05-03
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References

  1. Adams M. H. 1959; Methods of study of bacterial viruses. In Bacteriophages pp 443–522 Edited by Adams M. H. New York: Wiley-Interscience;
    [Google Scholar]
  2. Blair J. E., Carr M. 1961; Lysogen in staphylococci. Journal of Bacteriology 82:984–993
    [Google Scholar]
  3. Bradley D. E. 1963; The structure of some staphylococcus and pseudomonas phages. Journal of Ultrastructure Research 8:552–565
    [Google Scholar]
  4. Bradley D. E., Kay D. 1960; The fine structure of bacteriophages. Journal of General Microbiology 23:553–563
    [Google Scholar]
  5. Brown D. T., Brown N. C., Burlingham B. T. 1972; Morphology and physical properties of Staphylococcus bacteriophage PI 1-M15. Journal of Virology 9:664–671
    [Google Scholar]
  6. Delbruck M. 1945; Effect of specific antisera on the growth of bacterial viruses (bacteriophages). Journal of Bacteriology 50:137–154
    [Google Scholar]
  7. De saxe M., Porthouse A. 1979; Gentamicin resistance in Staphylococcus aureus. Lancet ii:370–371
    [Google Scholar]
  8. Gordon C. N., Kleinschmidt A. K. 1968; High contrast staining of individual nucleic acid molecules. Biochimica et Biophysica Acta 155:305–307
    [Google Scholar]
  9. Inoue M., Oshima H., Saito T., Okubo T., Mitsuhashi S. 1976; Integration of the erythromycin(ero) resistance gene of a staphylococcal plasmid into SI ppen.ion.phage. Virology 73:295–298
    [Google Scholar]
  10. Kondo I. 1973; Lysogenic conversion of /Mtemolysine and staphylokinase by staphylococcal phage of serotype F. Genetics 74:143–144
    [Google Scholar]
  11. Mitsuhashi S., Oshima H., Kuwaharada U., Hashimoto H. 1965; Drug-resistance of staphylococci. I. Transduction of tetracycline resistance with phage lysates obtained from multiply resistant staphylococci. Journal of Bacteriology 89:967–976
    [Google Scholar]
  12. Nonomura Y., Blobel G., Sabatini D. 1971; Structure of liver ribosomes studied by negative staining. Journal of Molecular Biology 60:303–323
    [Google Scholar]
  13. Rosenblum E. D., Tyrone S. 1964; Serology, density and morphology of staphylococcal phages. Journal of Bacteriology 88:1737–1742
    [Google Scholar]
  14. Saigo K. 1978; Isolation of high-density mutants and identification of nonessential structural proteins in bacteriophage T5: dispensability of L-shaped tailfibers and a secondary major head protein. Virology 85:422–433
    [Google Scholar]
  15. Saito T., Osono T., Inoue M., Mitsuhashi S. 1974; Morphological studies of lysogenic phages from S. aureus. Presented at the 32nd general meeting of the Kanto branch of the Japan Bacteriological Society, November 20 Tokyo:
    [Google Scholar]
  16. Seto J. T., Kaesberg P., Wilson J. B. 1956; Electron microscopy and serology of staphylococcal phage. Journal of Bacteriology 72:847–850
    [Google Scholar]
  17. Simon L. D., Anderson T. F. 1967; The infection of Escherichia coli by T2 and T4 bacteriophages as seen in the electron microscope. II. Structure and function of the baseplate. Virology 32:298–305
    [Google Scholar]
  18. Szybalski W., Szybalski E. H. 1971; Equilibrium density gradient centrifugation. Procedures in Nucleic Acid Research vol 2311–354 Edited by Cantoni G. L., Davis D. R. New York: Harper & Row;
    [Google Scholar]
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