1887

Abstract

SUMMARY

The WC11 isolate of malignant catarrhal fever virus (MCFV) was purified by a method employing phase separation of the virus in an aqueous polymer system. Virus was grown in primary bovine thyroid cells. The virus released into the extracellular medium was purified. Initial concentration and partial purification of MCFV occurred after separation of the virus into the dextran phase following the addition of 10% (w/v) polyethylene glycol and 8% (w/v) dextran T10 to the extracellular virus. Further purification was achieved by centrifugation into a 20% (w/v) Ficoll cushion followed by centrifugation to equilibrium by flotation in a 15 to 36% (w/w) CsCl or 30 to 60% (w/w) sucrose gradient. Virus recovery was monitored using a plaque assay on bovine thyroid cells and ranged from 3 to 23% of the input extracellular virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-59-1-101
1982-03-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/59/1/JV0590010101.html?itemId=/content/journal/jgv/10.1099/0022-1317-59-1-101&mimeType=html&fmt=ahah

References

  1. Adams A. 1973; Concentration of Epstein-Barr virus from cell culture fluids with polyethylene glycol. Journal of General Virology 20:391–394
    [Google Scholar]
  2. Albertsson P. A. 1967; Two-phase separation of viruses. Methods in Virology 2:303–320
    [Google Scholar]
  3. Barnhart E. R., Ash R. J. 1975; Physical characteristics of herpesvirions: low temperature and osmotic shock studies. Virology 66:563–567
    [Google Scholar]
  4. Brenner S., Horne R. W. 1959; A negative staining method for high resolution electron microscopy of viruses. Biochimica et Biophysica Acta 34:103–110
    [Google Scholar]
  5. Brunette D. M., Till J. E. 1971; A rapid method for the isolation of L-cell surface membranes using an aqueous two-phase polymer system. Journal of Membrane Biology 5:215–224
    [Google Scholar]
  6. Ferris D. H., Hamdy F. M., Dardiri A. H. 1976; Detection of African malignant catarrhal fever viral antigen in cell cultures by immunofluorescence. Veterinary Microbiology 1:437–438
    [Google Scholar]
  7. Gomori G. 1955; Preparation of buffers for use in enzyme studies. Methods in Enzymology 1:138–146
    [Google Scholar]
  8. Herpesvirus Study Group, International Committee for the nomenclature of viruses 1973; Provisional labels for herpesviruses. Journal of General Virology 20:417–419
    [Google Scholar]
  9. Kemp M. C., Perdue M. L., Rogers H. W., O’Callagan D. L., Randall C. C. 1974; Structural polypeptides of the hamster strain of equine herpesvirus type 1: products associated with purification. Virology 61:361–375
    [Google Scholar]
  10. Lawrence W. C. 1976; Purification of equine herpes type 1. Journal of General Virology 31:81–91
    [Google Scholar]
  11. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  12. McSharry J., Benzinger R. 1970; Concentration and purification of vesicular stomatitis virus by polyethylene glycol ‘precipitation’. Virology 40:745–746
    [Google Scholar]
  13. Mirchamsy J., Rapp F. 1968; A new overlay medium for plaquing animal viruses. Proceedings of the Society for Experimental Biology and Medicine 129:13–17
    [Google Scholar]
  14. Perdue M. L., Kemp M. C., Randall C. C., O’Callagan D. J. 1974; Studies of the molecular anatomy of the L-M cell strain of equine herpes virus type 1: proteins of the nucleocapsid and intact virion. Virology 59:201–216
    [Google Scholar]
  15. Phillipson L. 1969 In Fundamental Techniques in Virology vol 1: pp 109–128 Edited by Habel K., Salzman N. P. New York: Academic Press;
    [Google Scholar]
  16. Plowright W. 1968; Malignant catarrhal fever. Journal of the American Veterinary Medical Association 152:795–804
    [Google Scholar]
  17. Plowright W., Ferris R. D., Scott G. R. 1960; Blue wildebeest and the aetiological agent of bovine malignant catarrhal fever in East Africa. Nature, London 188:1167–1169
    [Google Scholar]
  18. Plowright W., Macadam R. F., Armstrong J. A. 1965; Growth and characterization of the virus of bovine malignant catarrhal fever in East Africa. Journal of General Microbiology 39:253–266
    [Google Scholar]
  19. Reid H. W., Plowright W., Rowe L. W. 1975; Neutralizing antibody to herpesviruses derived from wildebeest and hartebeest in wild animals in East Africa. Research in Veterinary Science 18:269–273
    [Google Scholar]
  20. Rossiter P. B., Mushi E. Z., Plowright W. 1977; The development of antibodies in rabbits and cattle infected experimentally with an African strain of malignant catarrhal fever virus. Veterinary Microbiology 2:57–66
    [Google Scholar]
  21. Schloer G. M., Hanson R. P. 1968; Plaque morphology of Newcastle disease virus as influenced by cell type and environmental factors. American Journal of Veterinary Research 29:283–295
    [Google Scholar]
  22. Shichijo A. 1976; Concentration and purification of Japanese encephalitis virus with aqueous polymer two-phase system. Tropical Medicine 18:1–10
    [Google Scholar]
  23. Spear P. G., Roizman B. 1972; Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpesvirion. Journal of Virology 9:143–159
    [Google Scholar]
  24. Svennerholm B., Vahlne A., Jeannson S., Lunden R., Olofson S., Svantesson G., Lycke E. 1980; Separation of herpes simplex virus virions and nucleocapsids on Percoll gradients. Journal of Virological Methods 1:303–309
    [Google Scholar]
  25. Vahlne A. G., Blomberg J. 1974; Purification of herpes simplex virus. Journal of General Virology 22:297–302
    [Google Scholar]
  26. Walter H., Krob E. J., Brooks D. E. 1978; Membrane surface properties other than charge involved in cell separation by partition in polymer, aqueous two-phase systems. Biochemistry 15:2959–2964
    [Google Scholar]
  27. Wibberley G. 1976; Observations on two strains of bovine malignant catarrhal fever virus in tissue culture. Research in Veterinary Science 21:105–107
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-59-1-101
Loading
/content/journal/jgv/10.1099/0022-1317-59-1-101
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error