1887

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Volume 59, Issue 1

Specificity and Properties of the Genome-linked Proteins of Nepoviruses Free

Abstract

SUMMARY

The infectivity of the RNA of six nepoviruses was decreased or abolished by proteinase K treatment, whereas that of the RNA of cowpea mosaic virus (comovirus group) or tomato bushy stunt virus was unaffected. The extent of the decrease in infectivity was characteristic for each nepovirus and was independent of the plant species used as virus source or as assay host. The infectivity of raspberry ringspot virus (RRV) RNA was less affected than that of the other nepoviruses but treatment with Pronase decreased infectivity more than treatment with proteinase K. Proteinase K treatment also abolished the infectivity for tobacco mesophyll protoplasts of RNA of tobacco ringspot virus (TRSV) and tomato black ring virus (TBRV).

Tests on virus RNA, labelled with I by the chloramine T method, provided evidence that three nepoviruses and Echtes Ackerbohnenmosaik-Virus (EAMV; comovirus group) have genome-linked proteins (VPg). Pronase treatment rendered about half (RNA of strawberry latent ringspot virus; SLRV), or nearly all (RNA of the other nepoviruses and EAMV), of the I soluble in 70% ethanol. Treatment of nepovirus RNA with ribonuclease P1 yielded a product with an estimated mol. wt. of 4000 ± 900. Mobilities in polyacrylamide gels of VPg from the RNA of different viruses differed slightly (SLRV > TBRV > TRSV > RRV). TRSV VPg yielded one I-labelled tryptic peptide whereas the genome-linked proteins of RRV and TBRV both yielded two major products, of which one was resistant to further digestion and the other was converted, apparently via intermediates, to a second more stable product. No difference was detected between the tryptic peptides obtained from VPg of different strains of RRV, or of TBRV, or between those obtained from RNA-1, RNA-2 or RNA-3 (satellite RNA) of TBRV. Nepovirus VPg is therefore virus-specific. It seems to be coded on RNA-1 and probably has multiple functions.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome-linked protein , nepovirus , protease and RNA infectivity
© Journal of General Virology 1982
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1982-03-01
2024-04-26
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References

  1. Burroughs J. N., Brown F. 1978; Presence of a covalently linked protein on calicivirus RNA. Journal of General Virology 41:443–446
     [Google Scholar]
  2. Cadman C. H. 1960; Studies on the relationship between soil-borne viruses of the ringspot type occurring in Britain and Continental Europe. Virology 11:653–664
     [Google Scholar]
  3. Chu P. W. G., Boccardo G., Francki R. I. B. 1981; Requirement of a genome-associated protein of tobacco ringspot virus for infectivity but not for in vitro translation. Virology 109:428–430
     [Google Scholar]
  4. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
     [Google Scholar]
  5. Daubert S. D., Bruening G. 1979; Genome associated proteins of comoviruses. Virology 98:246–250
     [Google Scholar]
  6. Daubert S. D., Bruening G., Najarian R. C. 1978; Protein bound to the genome RNAs of cowpea mosaic virus. European Journal of Biochemistry 92:45–51
     [Google Scholar]
  7. Dunsworth-Browne M., Schell R. E., Berk A. J. 1980; Adenovirus terminal protein protects single stranded DNA from digestion by a cellular exonuclease. Nucleic Acids Research 8:543–554
     [Google Scholar]
  8. Flanegan J. B., Pettersson R. F., Ambros V., Hewlett M. J., Baltimore D. 1977; Covalent linkage of a protein to a defined nucleotide sequence at the 5′-terminus of virion and replicative intermediate RNAs of poliovirus. Proceedings of the National Academy of Sciences of the United States of America 74:961–965
     [Google Scholar]
  9. Fraker P. J., Speck J. C. 1978; Protein and cell membrane iodinations with a sparingly soluble chloramide, 1, 3, 4, 6-tetrachloro-3u, 6a-diphenylglycoluril. Biochemical and Biophysical Research Communications 80:849–857
     [Google Scholar]
  10. Ghosh A., Dasgupta R., Salern-oRife T., Rutgers T., Kaesberg P. 1979; Southern bean mosaic viral RNA has a 5′-linked protein but lacks 3′-terminal poly(A). Nucleic Acids Research 7:2137–2146
     [Google Scholar]
  11. Goldbach R., Rezelman G., Van Kammen A. 1980; Independent replication and expression of B-component RNA of cowpea mosaic virus. Nature, London 286:297–300
     [Google Scholar]
  12. Golini F., Nomoto A., Wimmer E. 1978; The genome-linked protein of picornaviruses. IV. Difference in the VPg’s of encephalomyocarditis virus and poliovirus as evidence that the genome-linked proteins are virus-coded. Virology 89:112–118
     [Google Scholar]
  13. Golini F., Semler B. L., Dorner A. J., Wimmer E. 1980; Protein-linked RNA of poliovirus is competent to form an initiation complex of translation in vitro. Nature, London 287:600–603
     [Google Scholar]
  14. Gooding G. V. 1963; Purification and serology of a virus associated with the grape yellow vein disease. Phytopathology 53:475–480
     [Google Scholar]
  15. Harris T. J. R. 1979; The nucleotide sequence at the 5′ end of foot-and-mouth disease virus RNA. Nucleic Acids Research 7:1765–1786
     [Google Scholar]
  16. Harrison B. D. 1958a; Raspberry yellow dwarf, a soil-borne virus. Annals of Applied Biology 46:221–229
     [Google Scholar]
  17. Harrison B. D. 1958b; Further studies on raspberry ringspot and tomato black ring, soil-borne viruses that affect respberry. Annals of Applied Biology 46:571–584
     [Google Scholar]
  18. Harrison B. D. 1958c; Relationship between beet ringspot, potato bouquet and tomato black ring viruses. Journal of General Microbiology 18:450–460
     [Google Scholar]
  19. Harrison B. D., Barker N. 1978; Protease-sensitive, structure needed for infectivity of nepovirus RNA. Journal of General Virology 40:711–715
     [Google Scholar]
  20. Harrison B. D., Murant A. F. 1977; Nepovirus group. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses No 185
     [Google Scholar]
  21. Harrison B. D., Woods R. D. 1966; Serotypes and particle dimensions of tobacco rattle viruses from Europe and America. Virology 28:610–620
     [Google Scholar]
  22. Hruby D. E., Roberts W. K. 1978; Encephalomyocarditis virus RNA. III. Presence of a genome-associated protein. Journal of Virology 25:413–415
     [Google Scholar]
  23. Jones A. T., Barker H. 1976; Properties and relationships of broad bean stain virus and Echtes Ackerbohnenmosaik-Virus. Annals of Applied Biology 83:231–238
     [Google Scholar]
  24. King A. M. Q., Sangar D. V., Harris T. J. R., Brown V. 1980; Heterogeneity of the genome-linked protein of foot-and-mouth disease virus. Journal of Virology 34:627–634
     [Google Scholar]
  25. Kitamura N., Adler C. J., Rothberg P. G., Martinko J., Nathenson S. G., Wimmer E. 1980; The genome-linked protein of picornaviruses. VII. Genetic mapping of poliovirus VPg by protein and RNA sequence studies. Cell 21:295–302
     [Google Scholar]
  26. Klootwijk J., Klein I., Zabel P., VanKammen A. 1977; Cowpea mosaic virus RNAs have neither mVGpppN …. nor mono-, di- or triphosphates at their 5′ ends. Cell l1:73–82
     [Google Scholar]
  27. Koenio I. 1979; ltude de quelques propri & 6s des RNA du tomato black ring virus. Diplome d’Études Approfondies de Biochimie, University of Strasbourg
     [Google Scholar]
  28. Kubo S., Harrison B. D., Robinson D. J., Mayo M. A. 1975a; Tobacco rattle virus in tobacco mesophyll protoplasts: infection and virus multiplication. Journal of General Virology 27:293–304
     [Google Scholar]
  29. Kubo S., Harrison A. D., Barker N. 1975b; Defined conditions for growth of tobacco plants as sources of protoplasts for virus infection. Journal of General Virology 28:255–257
     [Google Scholar]
  30. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
     [Google Scholar]
  31. Lee Y. F., Nomoto A., Detjen B. M., Wimmer E. 1977; A protein covalently linked to poliovirus genome RNA. Proceedings of the National Academy of Sciences of the United States of A merica 74:59–63
     [Google Scholar]
  32. Lister A. M. 1964; Strawberry latent ringspot: a new nematode-borne virus. Annals of Applied Biology 54:167–176
     [Google Scholar]
  33. Lister R. M., Raniere L. C., Varney E. H. 1963; Relationships of viruses associated with ringspot diseases of blueberry. Phytopathology 53:1031–1035
     [Google Scholar]
  34. Maule A. J., Boulton M. I., Edmunds C., Wood K. A. 1980; Polyethylene glycol-mediated infection of cucumber protoplasts by cucumber mosaic virus and virus RNA. Journal of General Virology 47:199–203
     [Google Scholar]
  35. Mayo M. A., Barker N., Harrison B. O. 1979a; Evidence for a protein covalently linked to tobacco ringspot virus RNA. Journal of General Virology 43:735–740
     [Google Scholar]
  36. Mayo M. A., Barker N., Harrison B. D. 1979b; Polyadenylate in the RNA of five nepoviruses. Journal of General Virology 43:603–610
     [Google Scholar]
  37. Muram A. E., Taylor C. E., Chambers J. 1968; Properties, relationships and transmission of a strain of raspberry ringspot virus infecting raspberry cultivars immune to the common Scottish strain. Annals of Applied Biology 61:175–186
     [Google Scholar]
  38. Murant A. E., Mayo M. A., Harrison B. D., Goold R. A. 1973; Evidence for two functional RNA species and a ‘satellite’ RNA in tomato black ring virus. Journal of General Virology 19:275–278
     [Google Scholar]
  39. Murant A. V., Taylor M., Duncan G. H., Raschke J. H. 1981; Improved estimates of molecular weight of plant virus RNA by agarose gel electrophoresis and electron microscopy after denaturation with glyoxal. Journal of General Virology 53:321–332
     [Google Scholar]
  40. Nomoto A., Imura N. 1979; A convenient sequencing method for 5′ protein-linked RNAs. Nucleic Acids Research 7:1233–1246
     [Google Scholar]
  41. Nomoto A., Kitamura N., Golint F., Wimmer E. 1977; The 5′-terminal structures of poliovirion RNA and poliovirus mRNA differ only in the genome-linked protein VPg. Proceedings of the National Academy of Sciences of the United States of America 74:5345–5349
     [Google Scholar]
  42. Pallansch M. A., Kew O. M., Palmenberg A. C., Golini F., Wimmer E., Rueckert R. R. 1980; Picornaviral VPg sequences are contained in the replicase precursor. Journal of Virology 35:414–419
     [Google Scholar]
  43. Perez-Bercoff R., Gander M. 1978; comment>In vitro translation of mengovirus RNA deprived of the terminally-linked (capping?) protein. FEBS Letters 96:306–312
     [Google Scholar]
  44. Randles J. W., Harrison B. D., Murant A. F., Mayo M. A. 1977; Packaging and biological activity of the two essential RNA species of tomato black ring virus. Journal of General Virology 36:187–193
     [Google Scholar]
  45. Roainson D. J. 1982; Apparent lack of nucleotide sequence homology between the satellite and genome RNA species of tomato black ring virus. Journal of General Virology 58:453–456
     [Google Scholar]
  46. Robinson O. J., Barker H., Harrison B. D., Mayo M. A. 1980; Replication of RNA-1 of tomato black ring virus independently of RNA-2. Journal of General Virology 51:317–326
     [Google Scholar]
  47. Sangar D. V., Rowlands D. J., Harris T. J. R., Brown F. 1977; Protein covalently linked to foot-and mouth disease virus RNA. Nature, London 268:648–650
     [Google Scholar]
  48. Sangar D. V., Black D. N., Rowlands D. J., Harris T. J. R., Brown F. 1980; Location of the initiation site for protein synthesis on foot-and-mouth disease virus RNA by in vitro translation of defined fragments of the RNA. Journal of Virology 33:59–68
     [Google Scholar]
  49. Schaffer F. L., Ehresmann D. W., Fretz M. K., Soergel M. E. 1980; A protein, VPg, covalently linked to 36S calicivirus RNA. Journal of General Virology 47:215–220
     [Google Scholar]
  50. Stanley J., Rottier P., Davies J. W., Zabel P., Van Kammen A. 1978; A protein linked to the 5′ termini of both RNA components of the cowpea mosaic virus genome. Nucleic Acids Research 5:4505–4522
     [Google Scholar]
  51. Stanley J., Goldbach R., Van Kammen A. 1980; The genome linked protein of cowpea mosaic virus is coded by RNA from the bottom component. Virology 106:180–182
     [Google Scholar]
  52. Van Kammen A., De Jager C. P. 1978; Cowpea mosaic virus. Commonwealth Mycological Institute Association of Applied Biologists Descriptions of Plant Viruses No 197
     [Google Scholar]
  53. Vartapetian A. B., Drygin Y. F., Chumakov K. M., Bogdanov A. A. 1980; The structure of the covalent linkage between proteins and RNA in encephalomyocarditis virus. Nucleic Acids Research 8:3729–3742
     [Google Scholar]
  54. Wodnar-Filipowicz A., Szczesna E., Zan Kowalczewska M., Muthukrishnan S., Szybiak U., Legocki A. B., Filipowicz W. 1978; 5′-terminal 7-methylguanosine and mRNA function. The effect of enzymatic decapping and of cap analogs on translation of tobacco mosaic-virus RNA and globin mRNA in vitro. European Journal of Biochemistry 92:69–80
     [Google Scholar]
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Specificity and Properties of the Genome-linked Proteins of Nepoviruses
J. Gen. Virol. 59, 149 (1982); https://doi.org/10.1099/0022-1317-59-1-149
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