1887

Abstract

SUMMARY

Lymphocyte stimulation responses to herpes antigens were studied using virus-infected X-irradiated cells. Rabbits were immunized with herpes simplex virus type 1 (strain HFEM) grown in RK13 cells. For stimulation assay BHK21 cells were X-irradiated (15000 rad) and infected with a high m.o.i. of a temperature-sensitive () mutant (N102) of HFEM strain at the non-permissive temperature (38.5 °C) of virus. Virus antigens were expressed on the infected cells and there was no leakage of infectious virus into the medium at 38.5 °C. T lymphocytes from rabbits immunized with herpes simplex virus were specifically activated by herpesvirus-infected X-irradiated cells; lymph node cells from rabbits immunized with RK13 cells and from non-immune rabbits showed no proliferative response.

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/content/journal/jgv/10.1099/0022-1317-59-2-415
1982-04-01
2024-05-02
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References

  1. Arvidson S., Holme T., Wadstrӧm T. 1971; Influence of cultivation conditions on the production of extracellular proteins by Staphylococcus aureus. Acta Pathologica et Microbiologica Scandinavica 79B:399–405
    [Google Scholar]
  2. Ciavarra R. P., Kang C. Y., Forman J. 1980; Vesicular stomatitis antigens recognized by cytotoxic cells: analysis with defective interfering particles are reconstituted membrane vesicles. Journal of Immunology 125:336–343
    [Google Scholar]
  3. Doherty P. C. 1980; Surveillance of self:CMI to virally modified cell surface defined operationally by the MHC. In Progress in Immunology vol 4: pp 563–576 Proceedings of the Fourth International Congress of Immunology
    [Google Scholar]
  4. Geder L., Skinner G. R. B. 1971; Differentiation between type 1 and type 2 strains of herpes simplex virus by an indirect immunofluorescent technique. Journal of General Virology 12:179–182
    [Google Scholar]
  5. Hallberg T., Haegert D., Clein G. P., Coombs R. R. A., Feinstein A., Gurner B. W. 1974; Observations On the mixed antiglobulin reaction as a test for immunoglobulin-bearing lymphocytes in normal persons and in patients with chronic lymphatic leukaemia. Journal of Immunological Methods 4:317–332
    [Google Scholar]
  6. Jacobs R. P., Aurelian L., Cole G. A. 1976; Cell-mediated immune response to herpes simplex virus. Type-specific lymphoproliferative responses in lymph nodes draining the site of primary infection. Journal of Immunology 116:1520–1525
    [Google Scholar]
  7. Kapoor A. K., Nash A. A. 1979; Possible suppressor cell activity of C3 + ve and Ig − ve lymphoid cells in rabbit system. In vitro lymphocyte stimulation to HSV type 1 antigens. Immunology Letters 1:125–128
    [Google Scholar]
  8. Kapoor A. K., Ling N. R., Bazeley M. 1978; Lymphocyte stimulation by herpes simplex virus (HSV) antigens. 1. Culture conditions, cell fractionation and presentation of HSV antigens. Cytobios 20:151–162
    [Google Scholar]
  9. Miller J. F. A. P. 1978; Restrictions imposed on T lymphocyte reactivities by the major histocompatibility complex: implications for T cell repertoire selection. Immunological Reviews 42:76–107
    [Google Scholar]
  10. Moorhead J. W. 1978; Tolerance and contact sensitivity to DNFB in mice. VIII. Identification of distinct T cell subpopulations that mediate in vivo and in vitro manifestations of delayed hypersensitivity. Journal of Immunology 120:137–144
    [Google Scholar]
  11. Nash A. A. 1976; Separation of lymphocyte sub-populations using antibodies attached to staphylococcal protein A-coated surfaces. Journal of Immunological Methods 12:149–161
    [Google Scholar]
  12. Rosenberg F. L., Wohlenberg C., Nahmias A. J., Notkins A. B. 1972; Differentiation of type 1 and type 2 herpes simplex virus by in vitro stimulation of immune lymphocytes. Journal of Immunology 109:413–414
    [Google Scholar]
  13. Sethi K. K., Stroehmann I., Brandis H. 1980; Human T-cell cultures from virus-sensitized donors can mediate virus-specific and HLA-restricted cell lysis. Nature, London 286:718–720
    [Google Scholar]
  14. Wildy P. 1954; The growth of herpes simplex virus. Australian Journal of Experimental Biology and Medical Science 32:605–619
    [Google Scholar]
  15. Zinkernagel R. M. 1978; Thymus and lymphohaemopoietic cells: their role in T cell maturation in selection of T cells’ H-2-restriction-specificity and H-2 linked Ir gene control. Immunological Reviews 42:224–270
    [Google Scholar]
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