1887

Abstract

Summary

Temporal exclusion and breakdown of superinfecting virus deoxyribonucleic acid were measured after infection with T 4 and T 5 bacteriophages of normal strains of and strains deficient in endonuclease-I. Bacteria deficient in endonuclease-I when infected with T4 phage excluded superinfecting T4 with little solubilization of the secondary DNA. With wild-type bacteria exclusion was accompanied by extensive superinfection breakdown, probably caused by the bacterial endonuclease-I. In bacteria infected by T5 phage, superinfecting T2 phages could be excluded even when deoxyribonucleic acid degradation was inhibited by maintaining a low [Mg] in the growth medium. In the presence of 0·01 -magnesium ions, both wild-type bacteria and bacteria deficient in endonuclease-I infected with T 5 phage produced extensive solubilization of the DNA of superinfecting T2 or T4 phages. A nuclease induced by T5 was probably partly responsible for the DNA breakdown which occurred in these conditions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-6-3-333
1970-03-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/6/3/JV0060030333.html?itemId=/content/journal/jgv/10.1099/0022-1317-6-3-333&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1959 Bacteriophages New York: Interscience;
    [Google Scholar]
  2. Burton K. 1956; A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochemical Journal 62:315
    [Google Scholar]
  3. Campbell A. 1967 In Molecular Genetics part 11 p. 323 Taylor J. H. Ed New York: Academic Press Inc;
    [Google Scholar]
  4. Crawford L. 1959; Nucleic acid metabolism in Escherichia coli infected with phage T5. Virology 7:359
    [Google Scholar]
  5. Dulbecco R. 1952; Mutual exclusion between related bacteriophages. Journal of Bacteriology 63:209
    [Google Scholar]
  6. Dürwald H., Hoffmann-Berling H. 1968; Endonuclease-I-deficient and ribonuclease-I-deficient Escherichia coli mutants. Journal of Molecular Biology 34:331
    [Google Scholar]
  7. Edgar R. s., Wood W. B. 1966; Morphogenesis of bacteriophage T4 in extracts of mutant infected cells. Proceedings of the National Academy of Science, United States of America 55:498
    [Google Scholar]
  8. Fessler L. I., Keleman M. V., Burton K. 1960; Synthesis of protein in a purine-requiring Escherichia coli infected with bacteriophage T2. Biochemical Journal 77:558
    [Google Scholar]
  9. French R. C., Graham A. F., Lesley S. M., Van Rooyen C. E. 1952; The contribution of phosphorus from T2r+ bacteriophage to progeny. Journal of Bacteriology 64:597
    [Google Scholar]
  10. Graham A. F. 1953; The fate of the infecting phage particle. Annales de l’Institut Pasteur, Paris 84:90
    [Google Scholar]
  11. Hershey A. D., Chase M. 1952; Independent functions of viral protein and nucleic acid in growth of bacteriophage. Journal of General Physiology 36:39
    [Google Scholar]
  12. Hershey A. D., Garen A., Fraser D. K. 1954 Carnegie Inst. Washington Year-book 53:210
    [Google Scholar]
  13. Kaiser A. D. 1968 In Methods in Enzymology XII part B p. 877 Colowick S. P., Kaplan N. O. Ed New York and London: Academic Press Inc;
    [Google Scholar]
  14. Lanni Y. T. 1968; First-Step-Transfer deoxyribonucleic acid of bacteriophage T5. Bacteriological Reviews 32:227
    [Google Scholar]
  15. Lanni Y. T., Mccorquodale D. J. 1963; DNA metabolism in T 5-infected Escherichia coli. Virology 19:72
    [Google Scholar]
  16. Lesley S. M., French R. C., Graham A. F., Van Rooyen C. E. 1951; Studies on the relationship between virus and host cell. II. The breakdown of T2r+ bacteriophage upon infection of its host, Escherichia coli. Canadian Journal of Medical Science 29:128
    [Google Scholar]
  17. Linn S., Arber W. 1968; Host specificity of DNA produced by Escherichia coli. X. In vitro restriction of phage fd replicative form. Proceedings of the National Academy of Science, United States of America 59:1300
    [Google Scholar]
  18. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin reagent. Journal of Biological Chemistry 193:265
    [Google Scholar]
  19. Buchanan Mccorquodale D. J. 1968; Patterns of protein synthesis in Ts-infected Escherichia coli. Journal of Biological Chemistry 243:2550
    [Google Scholar]
  20. Meselson M., Yuan R. 1968; DNA restriction enzyme from Escherichia coli. Nature; London: 2171110
    [Google Scholar]
  21. Paul A. V., Lehman I. R. 1966; The deoxyribonucleases of Escherichia coli. VII. A deoxyribonuclease induced by infection with bacteriophage T 5. Journal of Biological Chemistry 241:3441
    [Google Scholar]
  22. Pfefferkorn E., Amos H. 1958; Deoxyribonucleic acid breakdown and resynthesis in T5 bacteriophage infection. Virology 6:299
    [Google Scholar]
  23. Sedat J., Sinsheimer R. L. 1964; Structure of the DNA of bacteriophage øX174. V. Purine sequences. Journal of Molecular Biology 9:489
    [Google Scholar]
  24. Stone A. B., Burton K. 1962; Studies on the deoxyribonucleases of bacteriophage-infected Escherichia coli. Biochemical Journal 85:600
    [Google Scholar]
  25. Weigle J. J., Delbrück M. 1951; Mutual exclusion between an infecting phage and a carried phage. Journal of Bacteriology 62:301
    [Google Scholar]
  26. Fielding P. E., Lunt M. R. 1969; A new deoxyribonuclease activity from bacteria infected with T5 bacteriophage. Federation of European Biochemical Societies Letters 5:214
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-6-3-333
Loading
/content/journal/jgv/10.1099/0022-1317-6-3-333
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error