1887

Abstract

SUMMARY

Cytopathological studies have shown that Yaba tumour pox virus (Yaba virus) infection leads to the accumulation of large lipid vacuoles. The rate of accumulation of these vacuoles increased as the infection proceeded. These lipid vacuoles were not seen in control cells or in cells infected with monkeypox virus (MPV) but were seen during Yaba virus infection in the presence of cytosine arabinofuranoside (100 μg/ml). Yaba virus also failed to inhibit host protein synthesis as infection proceeded for prolonged periods. Yaba virus proteins were shown to be substantially different from those of MPV when analysed by two-dimensional electrophoresis. The genome of Yaba virus gave restriction enzyme fragments which differed from those of the MPV genome when cleaved with the enzymes III and I. However, Yaba virus DNA hybridized to the dIII fragments K, L and M and to the I fragments A, B, C, E and G of MPV DNA.

Keyword(s): DNA hybridization , lipids and proteins
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1982-10-01
2024-05-02
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References

  1. Ambrus J. L., Peltz E. T., Grace J. T., Owens G. 1963; A virus induced tumor in primates. National Cancer Institute Monograph 10:447–458
    [Google Scholar]
  2. Andrewes C. H., Allison A. C., Armstrong J. A., Bearcroft G., Noven J. S. F., Pereira H. S. 1959; A virus disease of monkeys causing large superficial growths. Acta Unionis internationalis contra cancrum 15:760–763
    [Google Scholar]
  3. Appleyard G., Westwood J. C. N. 1964; The growth of rabbitpox virus in tissue culture. Journal of General Microbiology 37:391–401
    [Google Scholar]
  4. Arita M., Tagaya I. 1980; Virion polypeptides of poxviruses. Archives of Virology 63:209–225
    [Google Scholar]
  5. Bearcroft W. C. C., Jamieson M. F. 1958; An outbreak of subcutaneous tumors in rhesus monkeys. Nature, London 182:195–196
    [Google Scholar]
  6. Behbehani A. M., Balano G. R., Kamitsuk P. S., Wenner H. A. 1968; Yaba tumor virus. I. Studies of pathogenesis and immunity. Proceedings of the Society for Experimental Biology and Medicine 129:556–561
    [Google Scholar]
  7. Dales S., Kajioka R. 1964; The cycle of multiplication of vaccinia virus in Earle’s strain L cells. I. Uptake and penetration. Virology 24:278–294
    [Google Scholar]
  8. Deharven E., Yohn D. S. 1966; The fine structure of the Yaba monkey tumor poxvirus. Cancer Research 26:995–1008
    [Google Scholar]
  9. Grace J. T. Jr, Mirand E. A. 1963; Human susceptibility to a simian tumor virus. Annals of the New York Academy of Sciences 108:1123–1128
    [Google Scholar]
  10. Harper L., Bedson H. S., Buchan A. 1979; Identification of Orthopoxviruses by polyacrylamide gel electrophoresis of intracellular polypeptides. Virology 93:435–144
    [Google Scholar]
  11. Kilpatrick D., Rouhandeh H. 1981; The polypeptides of monkeypox virus. I. Analysis of the polypeptide synthesis of MPV by SDS-PAGE and by two dimensional electrophoresis. Virology 110:455–465
    [Google Scholar]
  12. Loening U. E. 1969; The determination of the molecular weight of ribonucleic acid by polyacrylamide gel electrophoresis. Biochemical Journal 113:131–138
    [Google Scholar]
  13. Lyles D. S., Randall C. C., White H. B. Jr 1975; An alteration in the kinetic properties of a cholesterogenic enzyme in fowlpox virus infection. Virology 66:106–116
    [Google Scholar]
  14. Mackett M., Archard L. C. 1979; Conservation and variation in Orthopoxvirus genome structure. Journal of General Virology 45:683–701
    [Google Scholar]
  15. Metzgar T. S., Grace J. T. Jr, Sproul E. E. 1962; Immunologic studies of subcutaneous virus-induced histiocytomas in primates. Annals of the New York Academy of Sciences 101:192–202
    [Google Scholar]
  16. Moss B. 1978 Poxviruses. In The Molecular Biology of Animal Viruses vol. 2 pp. 849–890 Edited by Nayak D. P. New York: Marcel Dekker;
    [Google Scholar]
  17. Muller H. K., Witter R., Schaffner W., Schumperli D., Menna A., Wyler R. 1977; Comparison of five poxvirus genomes by analysis with restriction endonucleases Hmdlll, BamI and EcoRI. Journal of General Virology 38:135–147
    [Google Scholar]
  18. Niven J. S. F., Armstrong J. A., Andrewes C. H., Pereira H. S., Valentine R. C. 1961; Subcutaneous ‘growth’ in monkeys produced by a poxvirus. Journal of Pathology and Bacteriology 81:1–14
    [Google Scholar]
  19. O’farrell P. 1975; High resolution two dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–1021
    [Google Scholar]
  20. Pennington T. H. 1974; Vaccinia virus polypeptide synthesis: sequential appearance and stability of pre- and post-replicative polypeptides. Journal of General Virology 25:433–444
    [Google Scholar]
  21. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  22. Schaffner W., Gross K., Telford J., Birnstiel M. L. 1976; Molecular analysis of the histone gene gluster of Psammechinus miliarus. II. The arrangement of the five histone-coding and spacer sequences. Cell 8:471–478
    [Google Scholar]
  23. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  24. Sproul E. E., Metzgar R. S., Grace J. T. Jr 1963; The pathogenesis of Yaba virus-induced histiocytomas in primates. Cancer Research 23:671–675
    [Google Scholar]
  25. Stabel S., Doerfler W., Friis R. R. 1980; Integration sites of adenovirus type 12 DNA in transformed hamster cells and hamster tumor cells. Journal of Virology 36:22–40
    [Google Scholar]
  26. Stern W., Dales S. 1974; Biogenesis of vaccinia: concerning the origin of the envelope phospholipids. Virology 62:293–306
    [Google Scholar]
  27. Tsuchiya Y., Tagaya I., Tsuruhara T. 1969; Titration and extensive serial passage of Yaba virus in vitro. Japanese Journal of Microbiology 13:103–117
    [Google Scholar]
  28. Wahl G. M., Stern M., Stark G. R. 1979; Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl paper and rapid hybridization by using dextran sulfate. Proceedings of the National Academy of Sciences of the United States of America 76:3683–3687
    [Google Scholar]
  29. Yohn D. S., Gallagher J. F. 1969; Some physical properties of Yaba poxvirus deoxyribonucleic acid. Journal of Virology 3:114–118
    [Google Scholar]
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