1887

Abstract

SUMMARY

The pH-dependent profiles of haemolysis by influenza viruses AHK/8/68 (HK virus) and APR/8/34 (PR8 virus) were found to possess characteristic differences. Both viruses were highly haemolytic at pH 5.0, but while HK virus-induced haemolysis was undetectable above pH 5.5 to 5.6, PR8 virus-induced haemolysis persisted at higher pH values, becoming undetectable above pH 5.7 to 5.8. In order to determine whether these haemolysis profiles were genetically determined, we studied the pH dependence of haemolysis by eight recombinants of HK and PR8 viruses. The pH profile of each recombinant clearly resembled that of one parent or the other, showing that it is an inherited trait. The pH profile was found to be conferred exclusively by the haemagglutinin (HA) gene.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-62-2-353
1982-10-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/62/2/JV0620020353.html?itemId=/content/journal/jgv/10.1099/0022-1317-62-2-353&mimeType=html&fmt=ahah

References

  1. Gomori G. 1955; Preparation of buffers. Methods in Enzymology 1:141
    [Google Scholar]
  2. Huang R. T. C., Rott R., Klenk H. D. 1981; Influenza viruses cause hemolysis and fusion of cells. Virologv 110:243–247
    [Google Scholar]
  3. Lenard J., Miller D. K. 1981; pH-dependent hemolysis by influenza, Semliki Forest virus, and Sendai virus. Virology 110:479–482
    [Google Scholar]
  4. Lenard J., Miller D. K. 1982a; Uncoating of enveloped viruses. Cell 28:5–6
    [Google Scholar]
  5. Lenard J., Miller D. K. 1982b Entry of enveloped viruses into cells. In Receptor-mediated Endocytosis and Processing Edited by Cuatrecasas P., Roth T. Receptors and Recognition Series, vol. 15 New York: Chapman and Hall (in press);
    [Google Scholar]
  6. Lubeck M. D., Schulman J. L., Palese P. 1978; Susceptibility of influenza A viruses to amantadine is influenced by the gene coding for M protein. Journal of Virology 28:710–716
    [Google Scholar]
  7. Maeda T., Ohnishi S. 1980; Activation of influenza virus by acidic media causes hemolysis and fusion of erythrocytes. FEBS Letters 122:283–287
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-62-2-353
Loading
/content/journal/jgv/10.1099/0022-1317-62-2-353
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error