1887

Abstract

SUMMARY

Two fowl plague virus temperature-sensitive () mutants belonging to different complementation groups were studied. Both were defective in the syntheses of unpolyadenylated complementary RNA and virus RNA (vRNA) at non-permissive temperature whereas primary transcription was unaffected. In addition, 29, in which the mutation is in gene 1 coding for polypeptide P, has a defect in ‘secondary’ synthesis of mRNA at non-permissive temperature whereas inhibition of m synthesis by 131, in which the mutation is in gene 2 coding for polypeptide P, appeared to result from a defect in v synthesis. These results indicate, therefore, that different enzymes are responsible for the syntheses of virus mRNAs and A(-)cRNAs, which is consistent with the apparent differences in initiation and termination of transcription in the two reactions. The patterns of synthesis of the various types of virus RNA during infection are discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-63-1-103
1982-11-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/63/1/JV0630010103.html?itemId=/content/journal/jgv/10.1099/0022-1317-63-1-103&mimeType=html&fmt=ahah

References

  1. Ghendon Y. Z., Markushin S. G., Marchenko A. T., Sitnikov B. S., Ginzburg V. P. 1973; Biochemical characteristics of fowl plague virus tsmutants. Virology 55:305–319
    [Google Scholar]
  2. Ghendon Y. Z., Markushin S. G., Blagoveshenskaya O. V., Ghenkina D. B. 1975; Study of fowl plague virus RNA synthesis in temperature-sensitive mutants. Virology 66:454–463
    [Google Scholar]
  3. Ghendon Y., Klimov A., Blagoveshenskaya O., Ghenkina D. 1979; Investigation of recombinants of human influenza and fowl plague viruses. Journal of General Virology 43:183–191
    [Google Scholar]
  4. Ghenkina D. B., Ghendon Y. Z. 1979; Recombination and complementation between orthomyxoviruses under conditions of abortive infection. Acta virologica 23:97–106
    [Google Scholar]
  5. Hay A. J., Lomniczi B., Bellamy A. R., Skehel J. J. 1977a; Transcription of the influenza virus genome. Virology 83:337–355
    [Google Scholar]
  6. Hay A. J., Bellamy A. R., Abraham G., Skehel J. J., Brand C. M., Webster R. G. 1977b; Procedures for characterization of the genetic material of candidate vaccine strains. Developments in Biological Standardization 39:15–24
    [Google Scholar]
  7. Hay A. J., Skehel J. J., Mccauley J. 1980; Structure and synthesis of influenza virus complementary RNAs. Philosophical Transactions of the Royal Society of London B288:341–348
    [Google Scholar]
  8. Hay A. J., Skehel J. J., Mccauley J. 1982; Characterization of influenza virus RNA complete transcripts. Virology 116:517–522
    [Google Scholar]
  9. Krug R. M., Ueda M., Palese P. 1975; Temperature-sensitive mutants of influenza WSN virus defective in virus-specific RNA synthesis. Journal of Virology 16:790–796
    [Google Scholar]
  10. Krug R. M., Broni B. A., Bouloy M. 1979; Are the 5' ends of influenza mRNAs synthesized in vivodonated by host cell mRNAs?. Cell 18:329–334
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  12. Lubeck M. D., Palese P., Schulman J. L. 1979; Nonrandom association of parental genes in influenza A virus recombinants. Virology 95:269–274
    [Google Scholar]
  13. Markushin S. G., Ghendon Y. Z. 1973; Genetic classification and biological properties of temperaturesensitive mutants of fowl plague virus. Acta virologica 17:369–376
    [Google Scholar]
  14. Mowshowitz S. L. 1978; PI is required for initiation of cRNA synthesis in WSN influenza virus. Virology 91:493–495
    [Google Scholar]
  15. Palese P., Ritchey M. B., Schulman J. L. 1977; P1 and P3proteins of influenza virus are required for complementary RNA synthesis. Journal of Virology 21:1187–1195
    [Google Scholar]
  16. Robertson H. D., Dickson E., Plotch S. J., Krug R. M. 1980; Identification of the RNA region transferred from a representative primer, globin mRNA, to influenza mRNA during in vitrotranscription. Nucleic Acids Research 8:925–942
    [Google Scholar]
  17. Russell W. C., Skehel J. J. 1972; The polypeptides of adenovirus-infected cells. Journal of General Virology 15:45–57
    [Google Scholar]
  18. Scholtissek C., Bowles A. L. 1975; Isolation and characterization of temperature-sensitive mutants of fowl plague virus. Virology 67:576–587
    [Google Scholar]
  19. Skehel J. J. 1973; Early polypeptide synthesis in influenza virus-infected cells. Virology 56:394–399
    [Google Scholar]
  20. Skehel J. J., Hay A. J. 1978; Nucleotide sequences at the 5'-termini of influenza virus RNAs and their transcripts. Nucleic Acids Research 5:1207–1219
    [Google Scholar]
  21. Smith G. L., Hay A. I. 1981 Synthesis of influenza virus RNAs. In The Replication of Negative Strand Viruses pp. 333–340 Edited by Bishop D. H. L., Compans R. W. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  22. Smith G. L., Hay A. J. 1982; Replication of the influenza virus genome. Virology 118:96–108
    [Google Scholar]
  23. Sugiura A., Ueda M., Tobita K., Enomoto C. 1975; Further isolation and characterization of temperaturesensitive mutants of influenza virus. Virology 65:363–373
    [Google Scholar]
  24. Ulmanen I., Broni B. A., Krug R. M. 1981; Role of two of the influenza virus core P proteins in recognising cap 1 structures (m7GPPNm) on RNAs and in initiating viral RNA transcription. Proceedings of the National Academy of Sciences of the United States of America 78:7355–7359
    [Google Scholar]
  25. Wolstenholme A. J., Barrett T., Nichol S. T., Mahy B. W. J. 1980; Influenza virus-specific RNA and protein syntheses in cells infected with temperature-sensitive mutants defective in the genome segment encoding nonstructural proteins. Journal of Virology 35:1–7
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-63-1-103
Loading
/content/journal/jgv/10.1099/0022-1317-63-1-103
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error