1887

Abstract

SUMMARY

Polyribosomal RNA from tobacco infected with sonchus yellow net virus was resolved by electrophoresis on agarose gels, transferred to nitrocellulose and hybridized to radioactive viral RNA. Polyadenylated RNA contained four major viral complementary species, molecular weights 2.2 × 10, 0.83 × 10, 0.62 × 10 and 0.46 × 10, plus a minor species, molecular weight approx. 3 × 10. No such species could be detected in the non-polyadenylated RNA fraction. The polyadenylated RNA from membrane-bound polyribosomes contained only the 0.83 × 10 and 0.46 × 10 molecular weight RNAs. The sizes of the RNAs were consistent with the expected sizes of messages for the virus proteins, calculated from the molecular weight of these proteins.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-11-2479
1983-11-01
2024-05-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/11/JV0640112479.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-11-2479&mimeType=html&fmt=ahah

References

  1. Bedbrook J. R., Smith S. M., Ellis J. 1980; Molecular cloning and sequencing of cDNA to the precursor to the small subunit of chloroplast ribuIose-1, 5-bisphosphate carboxylase. Nature, London 287:692–697
    [Google Scholar]
  2. Freeman G. J., Rose J. K., Clinton M., Huang A. S. 1977; RNA synthesis of vesicular stomatitis virus. VII. Complete separation of the mRNAs of vesicular stomatitis virus by duplex formation. Journal of Virology 21:1094–1104
    [Google Scholar]
  3. Grubman M. J., Moyer S. A., Bannerjee A. K., Ehrenfeld E. 1975; Sub-cellular localisation of vesicular stomatitis virus messenger RNAs. Biochemical and Biophysical Research Communications 62:531–538
    [Google Scholar]
  4. Gustafson G. D., Milner J. J., Mcfarland J. E., Pederson K., Larkins B. A., Jackson A. O. 1982; Investigation of the complexity of barley stripe mosaic virus RNAs with recombinant DNA clones. Virology 120:182–193
    [Google Scholar]
  5. Jackson A. O. 1978; Partial characterisation of the structural proteins of sonchus yellow net virus. Virology 87:172–181
    [Google Scholar]
  6. Jackson A. O., Christie S. R. 1977; Purification and some physicochemical properties of sonchus yellow net virus. Virology 77:344–355
    [Google Scholar]
  7. Jackson A.O., Larkins B. A. 1976; Influence of ionic strength, pH and chelation of divalent metal ions on isolation of polysomes from tobacco. Plant Physiology 57:5–10
    [Google Scholar]
  8. Krystosek A., Cawthon M. L., Rabat D. 1975; Improved methods for purification and assay of eukaryotic messenger ribonucleic acid and ribosomes. Journal of Biological Chemistry 250:6077–6084
    [Google Scholar]
  9. Mcmaster G. K., Carmichael G. C. 1977; Analysis of single-and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences, U,. S,. A 74:4835–4838
    [Google Scholar]
  10. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  11. Milner J. J., Jackson A. O. 1979; Sequence complementarity of sonchus yellow net virus RNA with RNA from polysomes of infected tobacco. Virology 97:90–99
    [Google Scholar]
  12. Milner J. J., Hakkaart M. J. J., Jackson A. O. 1979; Subcellular distribution of sequences complementary to sonchus yellow net virus RNA. Virology 98:497–501
    [Google Scholar]
  13. Morrison T. G. 1979; Rhabdovirus assembly and intracellular processing of viral components. In The Rhabdoviruses vol II: pp 95–114 Edited by Bishop D. H. L. Boca Raton: C.R.C. Press;
    [Google Scholar]
  14. Morrison T. G., Lodish H. F. 1975; Site of synthesis of membrane and non-membrane proteins of vesicular stomatitis virus. Journal of Biological Chemistry 250:6955–6962
    [Google Scholar]
  15. Peters D. 1981; Plant rhabdovirus group. Commonwealth Mycological Institute/ Association of Applied Biologists Descriptions of Plant Viruses No. 244
    [Google Scholar]
  16. Rave N., Crkvenjakov R., Boedtker H. 1979; Identification of procollagen mRNAs transferred to diazobenzyloxymethyl paper from formaldehyde-agarose gels. Nucleic Acids Research 6:3559–3567
    [Google Scholar]
  17. Repik P. 1979; Adsorption, penetration, and the in vivo mRNA transcription process. In The Rhabdoviruses vol II: pp 1–23 Edited by Bishop D. H. L. Boca Raton: C.R.C. Press;
    [Google Scholar]
  18. Rosbash M., Penman S. 1971; Membrane-associated protein synthesis in mammalian cells. I. The two classes of membrane-associated ribosomes. Journal of Molecular Biology 59:227–241
    [Google Scholar]
  19. Rose J. K., Knipe D. 1975; Nucleotide sequence complexities, molecular weights and poly(A) content of vesicular stomatitis virus mRNA species. Journal of Virology 15:992–1003
    [Google Scholar]
  20. Tesh R. B., Travassos DA Rosa A. P. A., Travassos da Rosa J.S. 1983; Antigenic relationship among rhabdoviruses infecting terrestrial vertebrates. Journal of General Virology 64:169–176
    [Google Scholar]
  21. Thomas P. S. 1980; Hybridisation of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U,. S,. A 77:5201–5205
    [Google Scholar]
  22. Toriyama S., Peters D. 1981; Differentiation between broccoli necrotic yellows virus and lettuce necrotic yellows virus by their transcriptase activities. Journal of General Virology 56:59–66
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-11-2479
Loading
/content/journal/jgv/10.1099/0022-1317-64-11-2479
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error