1887

Abstract

SUMMARY

Treatment of Daudi cells with successively increasing concentrations of interferon-α resulted in the selection of a cell population which multiplied in the continued presence of 10 units/ml of interferon-α. A number of clones of interferon-resistant Daudi cells were isolated from this population. Two clones, DIF and DIF, were found to exhibit moderate and pronounced resistance, respectively, to both the antiviral and antiproliferative actions of human interferons-α and -β. These clones were also less responsive to the enhancement by interferon of Epstein—Barr virus early antigen expression. Both the surface antigens and karyotype of the interferon-resistant clones were similar to those of parental Daudi cells. After prolonged cultivation in the absence of interferon, DIF cells were found to ‘revert’ to an intermediate interferon sensitivity. The interferon sensitivity of clone DIF remained unchanged even after more than 1 year in culture.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-12-2641
1983-12-01
2024-05-07
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/12/JV0640122641.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-12-2641&mimeType=html&fmt=ahah

References

  1. Adams A., Strander H., Cantell K. 1975; Sensitivity of the Epstein-Barr virus-transformed human lymphoid cell lines to interferon. Journal of General Virology 28:207–217
    [Google Scholar]
  2. Affabris Emma C., Rossi G. B. 1982; Isolation of interferon-resistant variants of Friend erythroleukemia cells: effects of interferon and ouabain. Virology 120:441–452
    [Google Scholar]
  3. Aguet M. 1980; High-affinity binding of125I-labelled mouse interferon to a specific cell surface receptor. Nature, London 284:459–61
    [Google Scholar]
  4. Aguet M., Blanchard B. 1981; High-affinity binding of 125I-labeled mouse interferon to a specific cell surface receptor. II. Analysis of binding properties. Virology 115:249–261
    [Google Scholar]
  5. Arce-Gomez B., Jones E. A., Barnstable C. J., Solomon E., BodmerK W. 1978; The genetic control of HLA-A and B antigens in somatic cell hybrids: requirement for β2 microglobulin. Tissue Antigens 11:96–112
    [Google Scholar]
  6. Bertino J. R., Srimatkandada S., Engel D., Medina W. D., Scheer D. I., Moroson B. A., Dube S. 1982; Gene amplification in a methotrexate-resistant human leukemia line K-562. In Gene Amplification pp 23–27 Edited by Schimke R. T. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  7. Borecky L., Fuchsberger N., Najnicka-Gondova V., Clampor R., Altaner C. 1977; The Cell-growth inhibitory effect of interferon. Studies of a resistant cell-subline. Neoplasma 24:125–137
    [Google Scholar]
  8. Bostock C. J., Tyler-Smith C. 1982; Amplification of dihydrofolate reductase genes and other DNA sequences in mouse cells. In Gene Amplification pp 15–21 Edited by Schimke R. T. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  9. Brown P. C., Kaufman R. J., Haber D., Kchimke R. T. 1982; Characteristics of dihydrofolate reductase gene amplification in murine and Chinese hamster ovary cell lines. In Gene Amplification pp 9–13 Edited by Schimke R. T. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. Chany C., Vignal M. 1970; Effect of prolonged interferon treatment on mouse embryonic fibroblasts transformed by murine sarcoma virus. Journal of General Virology 7:203–210
    [Google Scholar]
  11. Chen H. Y., Sato T., Fuse A., Kuwata T., Content J. 1981; Resistance to interferon of human adenocarcinoma cell line, HEC-1, and its sensitivity to natural killer cell action. Journal of General Virology 52:177–181
    [Google Scholar]
  12. Cooper J. E. K. 1973; 5-A single cell clones. In Tissue Culture Methods and Application pp 266–269 Edited by Kruse P. F. Jr, Patterson M. K. Jr New York & London: Academic Press;
    [Google Scholar]
  13. Gresser I., Bandu M.-T., Brouty-Boye D. 1974; Interferon and cell division. IX. Interferon-resistant L1210 cells: characteristics and origin. Journal of the National Cancer Institute 52:553–559
    [Google Scholar]
  14. Hilfenhaus J. 1976; Propagation of Semliki Forest virus in various human lymphoblastoid cell lines. Journal of General Virology 33:539–542
    [Google Scholar]
  15. Klein E., Klein G., Nadkarni J. S., Nadkarni J. J., Wigzell H., Clifford P. 1968; Surface IgM kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Research 28:1300–1310
    [Google Scholar]
  16. Kuwata T., Fuse A., Morinaga N. 1976; Effect of interferon upon cell and virus growth in transformed human cell lines. Journal of General Virology 33:7–15
    [Google Scholar]
  17. Masters J., Keeley B., Gay H., Attardi G. 1982; Variable content of double minute chromosomes is not correlated with degree of phenotype instability in methotrexate-resistant human cell lines. Molecular and Cellular Biology 2:498–507
    [Google Scholar]
  18. Mogensen K. E., Bandu M.-T., Vignaux F., Aguet M., Kresser I. 1981; Binding of 125I-labelled human α-interferon to human lymphoid cells. International Journal of Cancer 28:575–582
    [Google Scholar]
  19. Mogensen K. E., Cantell K. 1977; Production and preparation of human leukocyte interferon. Pharmacology and Therapeutics C 1:369–381
    [Google Scholar]
  20. Mogensen K. E., Cantell K. 1979; Non-specific adsorption of interferon to immobilized serum immune-globulin. Journal of General Virology 45:171–175
    [Google Scholar]
  21. Rosa F., Fellous M., Dron M., Tovey M. G., Revel M. 1982; Presence of an abnormal β2-microglobulin mRNA in Daudi cells: induction by interferon. Immunogenetics 17:125–131
    [Google Scholar]
  22. Silverman R. H., Watling D., Balkwill F. R., Trowsdale J., Kerr I. M. 1982; The ppp(A2′p)nA and protein kinase systems in wild-type and interferon-resistant Daudi cells. European Journal of Biochemistry 126:333–341
    [Google Scholar]
  23. Tomita Y., Cantell K., Kuwata T. 1982; Effects of human ** interferon on cell growth, replication of virus and induction of 2′–5′ oligoadenylate synthetase in three human lymphoblastoid cell lines and K562 cells. International Journal of Cancer 30:161–165
    [Google Scholar]
  24. Tovey M. G., Dron M., Gresser I. 1982; Interferon enhances the expression of Epstein-Barr virus early antigen in Daudi cells. Journal of General Virology 60:31–38
    [Google Scholar]
  25. Tovey M. G., Dron M., Mogensen K. E., Lebleu B., Mechti N., Begon-Lours-Guymarho J. 1983; Isolation of Daudi cells with reduced sensitivity to interferon. II. On the mechanisms of resistance. Journal of General Virology 64:2649–2653
    [Google Scholar]
  26. Verhaegen-Lewalle M., Kuwata T., Zhang Z.-X., Declercq E., Cantell K., Content J. 1982; 2-5A synthetase activity induced by interferon α, β and γ in human cell lines differing in their sensitivity to the anticellular and antiviral activities of these interferons. Virology 117:425–434
    [Google Scholar]
  27. Weissenbach J., Zeevi M., Landau T., Revel M. 1979; Identification of the translation products of human fibroblast interferon mRNA in reticulocyte lysates. European Journal of Biochemistry 98:7–8
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-12-2641
Loading
/content/journal/jgv/10.1099/0022-1317-64-12-2641
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error