1887

Abstract

SUMMARY

The virulent Venezuelan equine encephalitis (VEE) virus strain, Trinidad donkey (TRD) and its vaccine derivative strain TC-83, demonstrated biological and biochemical differences in their replication. These two viruses had similarly shaped growth curves; however, TRD virus-infected cells produced significantly more infectious virus than did the TC-83 virus-infected cells during the very early period of the replication cycle. TRD virus inhibited host cell protein synthesis in Vero cells earlier than did TC-83 virus as measured by the incorporation of [S]methionine into cellular proteins. Virus-specified proteins were detected 1 to 2 h earlier in TRD virus-infected cells than in the TC-83 virus-infected cells; however, pulse-chase studies failed to show differences in the processing of the viral structural proteins in cells infected by either of the viruses. TRD virus-infected cells produced more virus RNA than did the TC-83 virus-infected cells, and analysis of the intracellular viral RNA species showed an increased synthesis of 26S RNA in the TRD virus-infected cells. The difference in amounts of 26S virus RNA produced was most pronounced early during the infection and may explain why TRD virus infection resulted in an earlier production of viral proteins and inhibition of cellular protein synthesis than was observed in the cells infected with the avirulent vaccine strain of VEE virus.

Keyword(s): replication , VEE and virulence
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/content/journal/jgv/10.1099/0022-1317-64-5-1111
1983-05-01
2024-04-27
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References

  1. Berge T. O., Banks I. S., Tigertt W. D. 1961; Attenuation of Venezulan equine encephalomyelitis virus by in vitro cultivation in guinea pig heart cells. American Journal of Hygiene 73:209–218
    [Google Scholar]
  2. Berger M. L. 1980; Humoral and cell-mediated immune mechanisms in production of pathology in avirulent Semliki Forest virus encephalitis. Infection and Immunity 30:244–253
    [Google Scholar]
  3. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled protein and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  4. Brugge J. S., Erikson R. L. 1977; Identification of a transformation-specific antigen induced by an avian sarcoma virus. Nature, London 269:346–348
    [Google Scholar]
  5. Clarke D. H., Casals J. 1958; Techniques for hemagglutination-inhibition with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  6. France J. K., Wyrick B. C., Trent D. W. 1979; Biochemical and antigenic comparisons of the envelope glycoproteins of Venezuelan equine encephalomyelitis virus strains. Journal of General Virology 44:725–740
    [Google Scholar]
  7. Hanon N. 1970; Cell surface antigen induced by Venezuelan equine encephalomyelitis virus. Infection and Immunity 2:713–715
    [Google Scholar]
  8. Hardy F. M., Brown A. 1961; Growth of Venezuelan equine encephalomyelitis virus in L cells. I. Growth in monolayer cultures. Journal of Bacteriology 81:20–27
    [Google Scholar]
  9. Jahrling P. B., Dendy E., Eddy G. A. 1974; Correlates to increased lethality of attenuated Venezuelan encephalitis virus vaccine for immunosuppressed hamsters. Infection and Immunity 9:924–930
    [Google Scholar]
  10. Jahrling P. B., Navarro E., Scherer W. F. 1976; Interferon induction and sensitivity as correlates to virulence of Venezuelan encephalitis viruses for hamsters. Archives of Virology 51:23–35
    [Google Scholar]
  11. Johnson K. M., Martin D. H. 1974; Venezuelan equine encephalitis. Advances in Veterinary Science and Comprehensive Medicine 18:79–116
    [Google Scholar]
  12. Kääriäinen L., Söderlund H. 1978; Structure and replication of α-viruses. Current Topics in Microbiology and Immunology 82:15–69
    [Google Scholar]
  13. Kennedy S. 1976; Sequence relationships between the genome and the intracellular RNA species of standard and defective SFV. Journal of Molecular Biology 108:491–551
    [Google Scholar]
  14. Keranen S., Kaariainen L. 1975; Proteins synthesized by Semliki virus and its 16 temperature-sensitive mutants. Journal of Virology 16:388–396
    [Google Scholar]
  15. Lachmi B. E., Kaariainen L. 1977; Control of protein synthesis in Semliki Forest virus-infected cells. Journal of Virology 22:142–149
    [Google Scholar]
  16. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  17. Leblanc P. A., Scherer W. F., Sussdorf D. H. 1978; Infection of congenitally athymic (nude) and normal mice with avirulent and virulent strains of Venezuelan encephalitis virus. Infection and Immunity 21:779–785
    [Google Scholar]
  18. Lust G. 1966; Alterations of protein synthesis in arbovirus-infected L cells. Journal of Bacteriology 91:1612–1617
    [Google Scholar]
  19. Madansky C. H., Bratt M. A. 1981; Noncytopathic mutants of Newcastle disease virus are defective in virus-specific RNA synthesis. Journal of Virology 37:317–327
    [Google Scholar]
  20. Mecham J. O., Trent D. W. 1982; A comparison of the 26S mRNAs and structural proteins of an equine virulent Venezuelan encephalitis virus and its vaccine derivative. Virology 121:251–261
    [Google Scholar]
  21. Monath T. P. 1976; Togaviruses, bunyaviruses and Colorado tick fever virus. In Manual of Clinical Immunology pp 456–462 Edited by Rose N. R., Freedman H. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  22. Mowshowitz D. 1973; Identification of polysomal RNA in BHK cells infected by Sindbis virus. Journal of Virology 11:535–543
    [Google Scholar]
  23. Mussgay M., Weibel J. 1962; Electron microscopic and biological studies on the growth of Venezuelan equine encephalitis virus in KB cells. Virology 16:52–62
    [Google Scholar]
  24. Randall R., Mills J. W. 1944; Fatal encephalitis in man due to the Venezuelan virus of equine encephalomyelitis in Trinidad. Science 99:225–226
    [Google Scholar]
  25. Saborio J. L., Pong S. A., Koch G. 1974; Selective and reversible inhibition of initiation of protein synthesis in mammalian cells. Journal of Molecular Biology 85:195–211
    [Google Scholar]
  26. Scherer W. F., Ellsworth C. A., Ventura A. K. 1971; Studies of viral virulence. II. Growth and adsorption curves of virulent and attenuated strains of Venezuelan encephalitis virus in cultured cells. American Journal of Pathology 62:211–219
    [Google Scholar]
  27. Simmons D. T., Strauss J. H. 1974; Replication of Sindbis virus. V. Polyribosomes and mRNA in infected cells. Journal of Virology 14:552–559
    [Google Scholar]
  28. Söderlund H. 1973; Kinetics of formation of the Semliki Forest virus nucleocapsid. Intervirology 1:354–361
    [Google Scholar]
  29. Söderlund H., Glanville N., Kaariainen L. 1974; Polysomal RNA in Semliki Forest virus-infected cells. Intervirology 2:100–113
    [Google Scholar]
  30. Stollar V. 1980; Defective interfering alphaviruses. In The Togaviruses: Biology, Structure, Replication pp 427–457 Edited by Schlesinger R. W. New York: Academic Press;
    [Google Scholar]
  31. Strauss J. H., Strauss E. G. 1977; Togaviruses. In The Molecular Biology of Animal Viruses pp 111–166 Edited by Nayak D. P. New York: Marcel Dekker;
    [Google Scholar]
  32. Trent D. W., Clewley J. P., France J. K., Bishop D. H. L. 1979; Immunochemical and oligonucleotide finger print analyses of Venezuelan equine encephalomyelitis complex viruses. Journal of General Virology 43:365–381
    [Google Scholar]
  33. Weber K., Osborn M. 1969; The reliability of molecular weight determination by dodecyl sulfate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  34. Wengler G., Wengler G. 1976; Protein synthesis in BHK-21 cells infected with Semliki Forest virus. Journal of Virology 17:10–19
    [Google Scholar]
  35. Zhdanov V. M., Yershov F. I., Uryvayev L. V. 1969; Ribonucleic acids synthesized in Venezuelan equine encephalitis virus-infected cells. Virology 38:355–358
    [Google Scholar]
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