1887

Abstract

SUMMARY

The structure and arrangement of the multiple provirus copies of avian sarcoma virus in a rat XC cell line were studied by restriction endonucleases. The following observations were made: (i) the majority of the proviruses integrated randomly with respect to cell DNA; (ii) no gross deletions or rearrangements in the proviruses were observed; (iii) two types of proviruses (type I and type II) could be distinguished on the basis of restriction endonuclease cleavage sites; (iv) the virus rescued from these cells was derived from type II provirus, which has a novel RI site between the and genes; (v) most of the provirus units contained the gene.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-9-1885
1983-09-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/9/JV0640091885.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-9-1885&mimeType=html&fmt=ahah

References

  1. Akiyama Y., Vogt P. K. 1979; Integration of different sarcoma virus genomes into host DNA: evidence against tandem arrangement and for shared integration sites. Proceedings of the National Academy of Sciences, U. S. A 76:2465–2469
    [Google Scholar]
  2. Battula N., Temin H. M. 1978; Sites of integration of infectious DNA of avian reticuloendotheliosis viruses in different avian cellular DNAs. Cell 13:387–398
    [Google Scholar]
  3. De lorbe W. J., Luciw P. A., Goodman H., Varmus H. E., Bishop J. M. 1980; Molecular cloning and characterization of avian sarcoma virus circular DNA molecules. Journal of Virology 36:50–61
    [Google Scholar]
  4. Gilmer T. M., Parsons I. T. 1979; Analysis of cellular integration sites in avian sarcoma virus-infected duck embryo cells. Journal of Virology 32:762–769
    [Google Scholar]
  5. Guntaka R. V., Mitsialis S. A. 1980; Cloning of avian tumor virus DNA fragments in plasmid pBR322: evidence for efficient transcription in E. coli from a virus-coded promoter. Gene 12:113–121
    [Google Scholar]
  6. Guntaka R. V., Richards O. C., Shank P. R., Rung H. J., Davidson N., Fritsch E., Bishop J. M., Varmus H. E. 1976; Covalently closed circular DNA of avian sarcoma virus: purification from nuclei of infected tumour cells and measurement by electron microscopy and gel electrophoresis. Journal of Molecular Biology 106:337–357
    [Google Scholar]
  7. Guntaka R. V., Rao P. Y., Mitsialis S. A., Katz R. A. 1980; Modification of avian sarcoma proviral DNA sequences in non-permissive XC cells but not in permissive chick cells. Journal of Virology 34:569–572
    [Google Scholar]
  8. Highfield P. E., Rafield L. F., Gilmer T. M., Parsons J. T. 1980; Molecular cloning of avian sarcoma virus closed circular DNA: structural and biological characterization of three recombinant clones. Journal of Virology 36:271–279
    [Google Scholar]
  9. Hill M., Hillová J., Dantchev D., Mariage R., Goubin G. 1974; Infectious viral DNA in Rous sarcoma virus-transformed non-producer and producer animal cells. Cold Spring Harbor Symposia on Quantitative Biology 39:1015–1025
    [Google Scholar]
  10. Hložánek I., Svoboda J. 1972; Characterization of viruses obtained after cell fusion or transfection of chicken cells with DNA from virogenic mammalian Rous sarcoma cells. Journal of General Virology 17:55–59
    [Google Scholar]
  11. Hughes S. H., Shank P. R., Spector D. H., Rung H. J., Bishop J. M., Varmus H. E., Vogt P. K., Breitman M. L. 1978; Proviruses of avian sarcoma virus are terminally redundant, coextensive with unintegrated linear DNA and integrated at many sites. Cell 15:1397–1410
    [Google Scholar]
  12. Kaufman R. J., Brown P. C., Schimke R. E. 1979; Amplified dihydrofolate reductase genes in unstably methotrexate-resistant cells are associated with double minute chromosomes. Proceedings of the National Academy of Sciences, U. S. A 76:5669–5673
    [Google Scholar]
  13. Khoury A. T., Hanafusa H. 1976; Synthesis and integration of viral DNA in chicken cells at different times after infection with various multiplicities of avian oncorna virus. Journal of Virology 18:383–400
    [Google Scholar]
  14. Levy J., Kazan P. M., Varmus H. E. 1974; The importance of DNA size for successful transfection of chicken embryo fibroblasts. Virology 61:297
    [Google Scholar]
  15. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  16. Sabran J. L., Hsu T. W., Yeater C., Kaji A., Mason W. S., Taylor J. M. 1979; Analysis of integrated avian RNA tumor virus DNA in transformed chicken, duck and quail fibroblasts. Journal of Virology 29:170–178
    [Google Scholar]
  17. Shank P. R., Hughes S. H., Kung H. J., Majors J. E., Quintrell N., Guntaka R. V., Bishop J. M., Varmus H. E. 1978; Mapping unintegrated avian sarcoma virus DNA: termini of linear DNA bear 300 nucleotides present once or twice in two species of circular DNA. Cell 15:1383–1395
    [Google Scholar]
  18. Šimkovič D., Valentová N., Thurzo V. 1962; An in vitro system for the detection of Rous sarcoma virus in the cells of the rat tumor XC. Neoplasma 8:104–108
    [Google Scholar]
  19. Stehelin D., Deng C. T., Varmus H. E., Bishop J. M. 1977; Detection and enumeration of transformation-defective strains of avian sarcoma virus with molecular hybridization. Virology 76:675–685
    [Google Scholar]
  20. Svoboda J. 1961; The tumorigenic action of Rous sarcoma in rats and the permanent production of Rous virus by the induced rat sarcoma XC. Folia biologica, Praha 7:46–60
    [Google Scholar]
  21. Svoboda J. 1962; Further findings on the induction of tumors by Rous sarcoma virus in rats and on the Rous virus-producing capacity of the induced tumors (XC) in chicks. Folia biologica, Praha 8:215–220
    [Google Scholar]
  22. Svoboda J. 1981; Helper virus-dependent RSV rescue from two lines of hamster cells transformed in vivo by XC RSV. International Journal of Cancer 27:857–864
    [Google Scholar]
  23. Svoboda J., Machala O., Hložánek I. 1967; Influence of Sendai virus on RSV formation in mixed culture of virogenic mammalian cells and chicken fibroblasts. Folia biologica, Praha 13:155–157
    [Google Scholar]
  24. Svoboda J., Hložánek I., Mach O., Michlova A., Riman J., Urbankova M. 1973; Transfection of chicken fibroblasts with single exposure to DNA from virogenic mammalian cells. Journal of General Virology 21:47–55
    [Google Scholar]
  25. Taylor J. M., Illmensee R. I., Summers J. 1976; Efficient transcription of RNA into DNA by avian sarcoma virus polymerase. Biochimica et biophysica acta 442:324–330
    [Google Scholar]
  26. Temin H. M. 1974; Relationships among RNA tumor viruses and cells: implication for the origin of viruses, the evolution of vertebrates and cellular genetic systems. Annual Review of Genetics 8:155–178
    [Google Scholar]
  27. Varmus H. E., Bishop J. M., Vogt P. K. 1973a; Appearance of virus-specific DNA in mammalian cells following transfection by Rous sarcoma virus. Journal of Molecular Biology 74:613–626
    [Google Scholar]
  28. Varmus H. E., Vogt P. K., Bishop J. M. 1973b; Integration of deoxyribonucleic acid specific for Rous sarcoma virus after infection of permissive and non-permissive hosts. Proceedings of the National Academy of Sciences, U. S. A 70:3067–3071
    [Google Scholar]
  29. Varmus H. E., Guntaka R. V., Deng C. T., Bishop J. M. 1974a; Synthesis, structure and function of avian sarcoma virus-specific DNA in permissive and non-permissive cells. Cold Spring Harbor Symposia on Quantitative Biology 39:987–996
    [Google Scholar]
  30. Varmus H. E., Guntaka R. V., Warner F., Heasley S., Bishop J. M. 1974b; Synthesis of viral DNA in the cytoplasm of duck embryo fibroblasts and in enucleated cells after infection by avian sarcoma virus. Proceedings of the National Academy of Sciences, U. S. A 71:387–391
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-9-1885
Loading
/content/journal/jgv/10.1099/0022-1317-64-9-1885
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error