1887

Abstract

SUMMARY

When chick embryo fibroblasts infected with high multiplicities of a non-revertible temperature-sensitive () mutant (53) of Newcastle disease virus (NDV) at the permissive temperature (34 °C) and incubated to allow the appearance of virus-induced proteins were shifted up to and incubated at the non-permissive temperature (42 °C), analysis of [H]leucine-labelled proteins by SDS-PAGE revealed a marked increase in cellular protein synthesis and a decline in viral synthesis, with an eventual return to an apparently uninfected state. Mutant 53 apparently ceased to suppress cellular protein synthesis, and translation of viral proteins was markedly inhibited. This was not observed in -infected cells, or in 53-infected cells maintained at the permissive temperature. Recovery from the infected state was inhibited by actinomycin D at any stage, but was not prevented by cycloheximide present immediately before and during temperature shift-up. Mutant 53 was shown to be RNA at 42 °C, although viral mRNAs synthesized at 34 °C are shown to be present throughout host recovery but in an inactive state.

Keyword(s): IFN , NDV , protein synthesis and ts
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/content/journal/jgv/10.1099/0022-1317-65-11-2049
1984-11-01
2024-05-17
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References

  1. Baglioni C., Maroney P. A. 1980; Mechanism of action of human interferons. Journal of Biological Chemistry 255:8390–8393
    [Google Scholar]
  2. Chambers P., Samson A. C. R. 1980; A new structural protein for Newcastle disease virus. Journal of General Virology 50:155–166
    [Google Scholar]
  3. Chambers P., Samson A. C. R. 1982; Non-structural proteins in Newcastle disease virus-infected cells. Journal of General Virology 58:1–12
    [Google Scholar]
  4. Dahlberg J. E. 1968 Physical and genetic properties of Newcastle disease virus Ph.D. thesis Purdue University;
    [Google Scholar]
  5. Dianzani F., Zucca M., Scupham A., Georgiades J. A. 1980; Immune and virus-induced interferons may activate cells by different derepressional mechanisms. Nature, London 283:400–402
    [Google Scholar]
  6. Granoff A. 1959; Studies on mixed infection with Newcastle disease virus. I. Isolation of Newcastle disease mutants and test for genetic recombination between them. Virology 9:636–648
    [Google Scholar]
  7. Harper D. R., Samson A. C. R., Lee C. M., Simon E. 1983; A temperature-sensitive mutant of Newcastle disease virus which is affected in both haemagglutinin-neuraminidase and matrix proteins. Journal of General Virology 64:2781–2784
    [Google Scholar]
  8. Jaye M. C., Godchaux W. III, Lucas-Lenard J. 1982; Further studies on the inhibition of cellular protein synthesis by vesicular stomatitis virus. Virology 116:148–162
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  10. Masters P. A., Samuel C. E. 1983; Mechanism of interferon action: inhibition of vesicular stomatitis virus replication in human amnion U cells by cloned human leukocyte interferon. II. Effect on viral macromolecular synthesis. Journal of Biological Chemistry 258:12026–12033
    [Google Scholar]
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