1887

Abstract

SUMMARY

Protein synthesis in interferon-treated HeLa cells infected by different animal RNA viruses has been studied. Synthesis of vesicular stomatitis virus (VSV) proteins was not detected in cells treated with concentrations of HuIFN-α(Ly) above 10 IU/ml. No specific inhibition of glycosylation of the G protein was observed. In addition, inhibition of host protein synthesis in IFN-treated cells occurred when high multiplicities of VSV were used, even though no viral protein synthesis was detected. For other viruses, such as Newcastle disease virus, Semliki Forest virus, encephalomyocarditis virus and poliovirus, treatment of cells with interferon also led to inhibition of viral protein synthesis. However, influenza virus and reovirus protein synthesis in interferon-treated cells stayed at control levels. The finding of viral translation in influenza virus and reovirus-infected cells treated with interferon suggests that, at least for these two systems, the antiviral state is not mediated by the bulk inhibition of viral protein synthesis through the dsRNA-activated protein kinase, or the 2′–5′ oligo(A) system.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-2-377
1984-02-01
2024-05-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/2/JV0650020377.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-2-377&mimeType=html&fmt=ahah

References

  1. Baglioni C. 1979; Interferon-induced enzymatic activities and their role in the antiviral state. Cell 17:255–264
    [Google Scholar]
  2. Baglioni C., Minks M. A., Maroney P. A. 1978; Interferon action may be mediated by activation of a nuclease by pppA2′p5′A2′p5′A. Nature, London 273:684–686
    [Google Scholar]
  3. Baxt B., Sonnabend J. A., Bablanian R. 1977; Effect of interferon on vesicular stomatitis virus transcription and translation. Journal of General Virology 35:325–329
    [Google Scholar]
  4. Benedetto A., Rossi G. B., Amici C., Belardelli F., Cioe L., Carruba G., Carrasco L. 1980; Inhibition of animal virus production by means of translation inhibitors unable to penetrate normal cells. Virology 106:123–132
    [Google Scholar]
  5. Carrasco L., Smith A. E. 1980; Molecular biology of animal virus infection. Pharmacology & Therapeutics 9:311–355
    [Google Scholar]
  6. De Ferra F., Baglioni C. 1981; Viral messenger RNA unmethylated in the 5′-terminal guanosine in interferon-treated HeLa cells infected with vesicular stomatitis virus. Virology 112:426–435
    [Google Scholar]
  7. Francoeur A. M., Stanners C. P. 1978; Evidence against the role of K + in the shut-off of protein synthesis by vesicular stomatitis virus. Journal of General Virology 39:551–554
    [Google Scholar]
  8. Friedman R. M. 1968; Inhibition of arbovirus protein synthesis by interferon. Journal of Virology 2:1081–1085
    [Google Scholar]
  9. Friedman R. M. 1977; Antiviral activity of interferons. Bacteriological Reviews 41:543–567
    [Google Scholar]
  10. Friedman R. M., Sonnabend J. A. 1965; Inhibition of interferon action by puromycin. Journal of Immunology 95:696–703
    [Google Scholar]
  11. Garry R. F., Waite M. R. F. 1979; Na+ and K+ concentrations and the regulation of the interferon system in chick cells. Virology 96:121–128
    [Google Scholar]
  12. Gupta S. L., Holmes S. L., Mehra L. L. 1982; Interferon action against reovirus: activation of interferon-induced protein kinase in mouse L929 cells upon reovirus infection. Virology 120:495–499
    [Google Scholar]
  13. Hovanessian A. G., Meurs E., Aujeau O., Vaquero C., Stefanos S., Falcoff E. 1980; Antiviral response and induction of specific proteins in cells treated with immune T (type II) interferon analogous to that from viral interferon (type I)-treated cells. Virology 104:195–204
    [Google Scholar]
  14. Jay F. T., Dawood M. R., Friedman R. M. 1983; Interferon induces the production of membrane protein-deficient and infectivity-defective vesicular stomatitis virions through interference in the virion assembly process. Journal of General Virology 64:707–712
    [Google Scholar]
  15. Joklik W. K. 1981; Structure and function of the reovirus genome. Microbiological Reviews 45:483–501
    [Google Scholar]
  16. Kimchi A., Shulman L., Schmidt A., Chernajovsky Y., Fradin A., Revel M. 1979; Kinetics of the induction of three translation-regulating enzymes by interferon. Proceedings of the National Academy of Sciences, U. S. A. 76:3208–3212
    [Google Scholar]
  17. Kitamura N., Semler B. L., Rothberg P. G., Larsen G. R., Adler C. J., Dorner A. J., Emini E. A., Hanecak R., Lee J. J., Van Der Were S., Anderson C. W., Wimmer E. 1981; Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature, London 291:547–553
    [Google Scholar]
  18. Kohn A. 1979; Early interactions of viruses with cellular membranes. Advances in Virus Research 24:223–276
    [Google Scholar]
  19. Lengyel P. 1982; Biochemistry of interferons and their actions. Annual Review of Biochemistry 51:251–282
    [Google Scholar]
  20. Lockart R. Z. Jr 1964; The necessity of cellular RNA and protein synthesis for viral inhibition from interferon. Biochemical and Biophysical Research Communications 15:513–518
    [Google Scholar]
  21. Lodish H. F., Porter M. 1980; Translational control of protein synthesis after infection by vesicular stomatitis virus. Journal of Virology 36:719–733
    [Google Scholar]
  22. Maheswari R. K., Banerjee D. K., Waetcher C. J., Olden K., Friedman R. M. 1980a; Interferon treatment inhibits glycosylation of a viral protein. Nature, London 287:454–456
    [Google Scholar]
  23. Maheswari R. K., Jay F. T., Friedman R. M. 1980b; Selective inhibition of glycoprotein and membrane protein of vesicular stomatitis virus from interferon-treated cells. Science 207:540–541
    [Google Scholar]
  24. Manders E. K., Tiller J. G., Huang A. S. 1972; Interferon-mediated inhibition of virion-directed transcription. Virology 49:573–581
    [Google Scholar]
  25. Marcus P. I., Sekellick M. J. 1978; Interferon action. III. The rate of primary transcription of vesicular stomatitis virus is inhibited by interferon action. Journal of General Virology 38:391–408
    [Google Scholar]
  26. Marcus P. I., Englehardt D. L., Hunt J. M., Sekellick M. J. 1971; Interferon action: inhibition of vesicular stomatitis virus RNA synthesis induced by virion-bound polymerase. Science 174:593–598
    [Google Scholar]
  27. Munoz A., Carrasco L. 1981; Protein synthesis and membrane integrity in interferon-treated HeLa cells infected with encephalomyocarditis virus. Journal of General Virology 56:153–162
    [Google Scholar]
  28. Olden K., Bernard B. A., Turner W., White S. C. 1982; Effect of interferon on protein glycosylation and comparison with tunicamycin. Nature, London 300:290–292
    [Google Scholar]
  29. Ratner L., Sen G. C., Brown G. E., Lebleu B., Kawakita M., Cabrer B., Slattery E., Lengyel P. 1977; Interferon double-stranded RNA and RNA degradation. European Journal of Biochemistry 79:565–577
    [Google Scholar]
  30. Repik P., Flamand A., Bishop D. H. 1974; Effect of interferon upon the primary and secondary transcription of vesicular stomatitis and influenza virus. Journal of Virology 14:1169–1178
    [Google Scholar]
  31. Revel M. 1979; Molecular mechanism involved in the antiviral effects of interferon. In Interferon 1 pp 102–163 Edited by Gresser I. London: Academic Press;
    [Google Scholar]
  32. Sen G. C. 1982; Mechanism of interferon action: progress towards its understanding. Progress in Nucleic Acids Research and Molecular Biology 27:105–156
    [Google Scholar]
  33. Simili M., De Ferra F., Baglioni C. 1980; Inhibition of RNA and protein synthesis in interferon-treated HeLa cells infected with vesicular stomatitis virus. Journal of General Virology 47:373–384
    [Google Scholar]
  34. II Stewart W. E. (editor) 1981 The Interferon System Wien; New York: Springer-Verlag:
    [Google Scholar]
  35. Taylor J. 1964; Inhibition of interferon action by actinomycin. Biochemical and Biophysical Research Communications 14:447–453
    [Google Scholar]
  36. Thacore H. R. 1978; Effect of interferon on transcription and translation of vesicular stomatitis virus in human and simian cell cultures. Journal of General Virology 41:421–426
    [Google Scholar]
  37. Williams B. R. G., Kerr I. A. 1980; The 2–5 A (pppA2′p5′A2′p5′A) system in interferon-treated and control cells. Trends in Biochemical Sciences 5:138–140
    [Google Scholar]
  38. Yamakazi S., Wagner R. R. 1970; Action of interferon: kinetics and differential effect on viral functions. Journal of Virology 6:421–429
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-2-377
Loading
/content/journal/jgv/10.1099/0022-1317-65-2-377
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error