1887

Abstract

Summary

Intracellular DNA-protein complexes (‘virosomes’) of vaccinia virus have been isolated. The solubilization of the ‘virosome’-bound DNA polymerase activity was attempted by a variety of high-salt extraction procedures. The most efficient of these used 0.3 -ammonium sulphate followed by brief sonication. The solubilized DNA polymerase activity from the ‘virosomes’, together with the DNA polymerases from 100000 supernatant fluids from the cytoplasm of infected and uninfected cells were chromatographed on DEAE-cellulose and their properties compared. The ‘virosome’ DNA polymerase activity differed from the soluble vaccinia virus-induced DNA polymerase activity in its requirements for divalent cations and in respect of pH optimum, for the deoxyribonucleoside triphosphates and the effect of -ethylmaleimide.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-4-825
1984-04-01
2024-05-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/4/JV0650040825.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-4-825&mimeType=html&fmt=ahah

References

  1. Bems K. I., Silverman C., Weissbach A. 1969; Separation of a new DNA polymerase from vaccinia-infected HeLa cells. Journal of Virology 4:15–23
    [Google Scholar]
  2. Cairns H. I. F. 1960; The initiation of vaccinia virus infection. Virology II:603–623
    [Google Scholar]
  3. Challberg M. D., Englund P. T. 1979; Purification and properties of the deoxyribonucleic acid polymerase induced by vaccinia virus. Journal of Biological Chemistry 254:7812–7819
    [Google Scholar]
  4. Chang L. M. S., Hodes M. E. 1969; Partial separation of the DNA nucleotidyltransferases of the Shope rabbit fibroma. Biochemical and Biophysical Research Communications 31:545–552
    [Google Scholar]
  5. Citarella R. V., Muller R., Schlabach A., Weissbach A. 1972; Studies on vaccinia virus-directed DNA polymerase. Journal of Virology 10:721–729
    [Google Scholar]
  6. Joklik W. K., Becker Y. 1964; The replication and coating of vaccinia DNA. Journal of Molecular Biology 10:452–474
    [Google Scholar]
  7. Jungwirth C., Joklik W. K. 1965; Studies on “early” enzymes in HeLa cells infected with vaccinia virus. Virology 27:80–93
    [Google Scholar]
  8. Lambert D. E., Magee W. E. 1977; Characterisation of vaccinia virus DNA replication in vitro. Virology 79:342–354
    [Google Scholar]
  9. Lewis B. J., Abrell J. W., Smith R. G., Gallo R. C. 1974; DNA polymerases in human lymphoblastoid cells infected with simian sarcoma virus. Biochimica et biophysica acta 349:148–160
    [Google Scholar]
  10. Loeb L. A. 1969; Purification and properties of deoxyribonucleic acid polymerase from nuclei of sea urchin embryos. Journal of Biological Chemistry 244:1672–1678
    [Google Scholar]
  11. Magee W. E., Miller O. V. 1967; Immunological evidence for the appearance of a new DNA polymerase in cells infected with vaccinia virus. Virology 31:64–69
    [Google Scholar]
  12. Martin R. G., Ames B. N. 1961; A method for determining the sedimentation behavior of enzymes: application to protein mixtures. Journal of Biological Chemistry 236:1372–1379
    [Google Scholar]
  13. Nowakowski M., Kates J. R., Bauer W. 1978; Isolation of two DNA-binding proteins from the intracellular replication complex of vaccinia virus. Virology 84:260–267
    [Google Scholar]
  14. Pedrali-noy G., Weissbach A. 1977; HeLa cell DNA polymerases: the effect of cycloheximide in vivo and detection of a new form of DNA polymerase alpha. Biochimica et biophysica acta 477:70–83
    [Google Scholar]
  15. Polisky B., Kates J. R. 1976; Interaction of vaccinia DNA-binding proteins with DNA in vitro. Virology 69:143–147
    [Google Scholar]
  16. Poulson R., Krasney J., Zbarsky S. H. 1973; Distribution and properties of DNA polymerase in subcellular fractions of rat intestinal mucosa. Biochimica et biophysica acta 299:533–544
    [Google Scholar]
  17. Roeder R. G., Rutter W. J. 1969; Multiple forms of DNA-dependent RNA polymerase in eukaryotic organisms. Nature, London 224:234–237
    [Google Scholar]
  18. Weissbach A., Schlabach A., Fridlender B., Bolden A. 1971; DNA polymerases from human cells. Nature New Biology 131:167–170
    [Google Scholar]
  19. Weissbach A., Baltimore D., Bollum F., Gallo R. C., Korn D. 1975; Nomenclature of eukaryotic DNA polymerases. Science 190:401–402
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-4-825
Loading
/content/journal/jgv/10.1099/0022-1317-65-4-825
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error