1887

Abstract

SUMMARY

Monoclonal antibodies were prepared from mice and rats immunized with Friend leukaemia virus and BALB/c xenotropic virus. By immunoprecipitation of I-labelled and [S]methionine-labelled viruses and by protein blotting, ten antibodies were found to react with the viral components p12, p15, p30, gp70 and p15E/p12E. A dot-immunobinding assay was found to be a reliable method to type the antibody reactivity with different murine leukaemia viruses (MuLVs). When tested on a panel of ecotropic and xenotropic MuLVs the antibodies revealed the following antigenic specificities: ecotrop-specific on p15E/p12E; xenotrop-specific on p15E; group-specific on p30 and p15E; FM-specific on gp70; FR-specific on gp70 and p15. Of particular interest is a cytotoxic antibody recognizing an FMR determinant localized on p12.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-9-1507
1984-09-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/9/JV0650091507.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-9-1507&mimeType=html&fmt=ahah

References

  1. Alkan S. S., Ball R. K., Chang J.-Y., Braun D. G. 1983; Heterogeneity of cross-reactive idiotypes: serological and structural analysis of monoclonal anti-p-azobenzene-arsonate antibodies expressing major and minor idiotypes. Molecular Immunology 20:203–211
    [Google Scholar]
  2. Amanuma H., Katori A., Obata M., Sagata N., Ikawa Y. 1983; Complete nucleotide sequence of the gene for the specific glycoprotein (gp55) of Friend spleen focus-forming virus. Proceedings of the National Academy of Sciences, U. S. A 80:3913–3917
    [Google Scholar]
  3. Chesebro B., Wehrly K., Cloyd M., Britt W., Portis J., Collins J., Nishio J. 1981; Characterization of mouse monoclonal antibodies specific for Friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens. Virology 112:131–144
    [Google Scholar]
  4. Chesebro B., Britt W., Evans L., Wehrly K., Nishio J., Cloyd M. 1983; Characterization of monoclonal antibodies reactive with murine leukemia viruses: use in analysis of strains of Friend MCF and Friend ecotropic murine leukemia virus. Virology 127:134–148
    [Google Scholar]
  5. Clark S. P., Mak T. W. 1983; Complete nucleotide sequence of an infectious clone of Friend spleen focus-forming provirus: gp55 is an envelope fusion glycoprotein. Proceedings of the National Academy of Sciences, U. S. A 80:5037–5041
    [Google Scholar]
  6. Cloyd M. W., Chesebro B., Portis J. L., Weir M. 1982; MCF-specific murine monoclonal antibodies made against AKR-247 MCF virus recognize a unique determinant associated with the gp70-p15(E) complex. Journal of Virology 41:1112–1117
    [Google Scholar]
  7. Friedman M., Lilly F., Nathenson S. G. 1974; Cell surface antigen induced by Friend murine leukemia virus is also in the virion. Journal of Virology 14:1126–1131
    [Google Scholar]
  8. Galfré G., Howes S. C., Milstein C., Butcher G. W., Howard J. C. 1977; Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature, London 266:550–552
    [Google Scholar]
  9. Gambke C. H., Deppert W. 1981; Late nonstructural 100.000-and 33.000-dalton proteins of adenovirus type 2.1. Subcellular localization during the course of infection. Journal of Virology 40:585–593
    [Google Scholar]
  10. Greenwood F. C., Hunter W. M., Glover J. S. 1963; The preparation of1311-labelled human growth hormone of high specific radioactivity. Biochemical Journal 89:114–123
    [Google Scholar]
  11. Hawkes R., Niday E., Gordon J. 1982; A dot-immunobinding assay for monoclonal and other antibodies. Analytical Biochemistry 119:142–147
    [Google Scholar]
  12. Hunsmann G., Moenning V., Pister L., Seifert E., Schafer W. 1974; Properties of mouse leukemia virus. VIII. The major viral glycoprotein of Friend leukemia virus. Seroimmunological, interfering and hemagglutinating capacities. Virology 62:307–318
    [Google Scholar]
  13. Koch W., Hunsmann G., Friedrich R. 1983; Nucleotide sequence of the envelope gene of Friend murine leukemia virus. Journal of Virology 45:1–9
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. Lostrom M. E., Stone M. R., Tam M., Burnette W. N., Pinter A., Nowinski R. C. 1979; Monoclonal antibodies against murine leukemia viruses: identification of six antigenic determinants on the pi5(E) and gp70 envelope proteins. Virology 98:336–350
    [Google Scholar]
  16. Maizel J. V. Jr 1971; Polyacrylamide gel electrophoresis of viral proteins. Methods in Virology 5:179–246
    [Google Scholar]
  17. Mann R., Mulligan R. C., Baltimore D. 1983; Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell 33:153–159
    [Google Scholar]
  18. Monckton P., Moroni C. 1980; Foetal calf serum acts as inducer of endogenous C-type virus in mouse lymphoid cells. Journal of General Virology 47:59–66
    [Google Scholar]
  19. Moroni C. 1972; Structural proteins of Rauscher leukemia virus and Harvey sarcoma virus. Virology 47:1–7
    [Google Scholar]
  20. Moroni C., Robert-Guroff M., Martin D. 1974; Virion and non-virion murine leukemia membrane antigen analysis with virus absorbed antisera. Intervirology 3:292–304
    [Google Scholar]
  21. Niman H. L., Elder J. H. 1980; Molecular dissection of Rauscher virus gp70 by using monoclonal antibodies: localization of acquired sequences of related envelope gene recombinants. Proceedings of the National Academy of Sciences, U. S. A 77:4524–4528
    [Google Scholar]
  22. Nowinski R. C., Emery S., Ledbetter J. 1978; Identification of an FMR cell surface antigen associated with murine leukemia virus-infected cells. Journal of Virology 26:805–812
    [Google Scholar]
  23. Nowinski R. C., Lostrom M. E., Tam M. R., Stone M. E., Burnette W. N. 1979; The isolation of hybrid cell lines producing monoclonal antibodies against the pi5(E) protein of ecotropic murine leukemia virus. Virology 93:111–126
    [Google Scholar]
  24. Nowinski R. C., Pickering R., O’Donnell P. V., Pinter A., Hammerling U. 1981; Selective neutralization of ecotropic murine leukemia virus by monoclonal antibodies: localization of a site on the gp70 protein associated with ecotropism. Virology 111:84–92
    [Google Scholar]
  25. O’donnell P., Nowinski R. C. 1980; Serological analysis of antigenic determinants on the env gene products of AKR dualtropic (MCF) murine leukemia viruses. Virology 107:81–88
    [Google Scholar]
  26. Old L. J., Oyse E. A. 1965; Antigens of tumors and leukemias induced by viruses. Federation Proceedings 24:1009–1017
    [Google Scholar]
  27. Old L. J., Boyse E. A., Lilly E. 1963; Formation of cytotoxic antibody against leukemias induced by Friend viruses. Cancer Research 23:1063–1068
    [Google Scholar]
  28. Pinter A., Fleissner E. 1977; The presence of disulfide-linked gp70-p15E complexes in AKR murine leukemia virus. Virology 83:417–422
    [Google Scholar]
  29. Pinter A., Honnen W. J., Tung J. S., O’Donnell P. V., Hammerling U. 1982; Structural domains of endogenous murine leukemia virus gp70s containing specific antigenic determinants defined by monoclonal antibodies. Virology 116:499–516
    [Google Scholar]
  30. Portis J. L., Mcatee F. J., Cloyd M. W. 1982; Monoclonal antibodies to xenotropic and MCF murine leukemia viruses derived during the graft-versus-host reaction. Virology 118:181–190
    [Google Scholar]
  31. Schneider J., Falk H., Hunsmann G. 1980; Envelope polypeptides of Friend leukemia virus: purification and structural analysis. Journal of Virology 33:597–605
    [Google Scholar]
  32. Seifert E., Claviez M., Frank H., Hunsmann G., Schwarz H., Schafer W. 1975; Production of substantial amounts of Friend leukemia virus by a suspension tissue culture line (Eveline suspension cells). Zeitschrift für Naturforschung 30c:698–700
    [Google Scholar]
  33. Shinnick T. M., Lerner R. A., Sutcliffe J. G. 1981; Nucleotide sequence of Moloney murine leukemia virus. Nature, London 293:543–548
    [Google Scholar]
  34. Shulman M., Wilde C. D., Kohler G. 1978; A better cell line for making hybridomas secreting specific antibodies. Nature, London 276:269–270
    [Google Scholar]
  35. Strand M., August J. T. 1973; Structural proteins of oncogenic ribonucleic acid viruses. Journal of Biological Chemistry 248:5627–5633
    [Google Scholar]
  36. Strand M., August J. T. 1975; Structural proteins of RNA tumor viruses as probes for viral gene expression. Cold Spring Harbor Symposia on Quantitative Biology 39:1109–1116
    [Google Scholar]
  37. Towbin H., Staeheun T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. ProceedingBs of the National Academy of Sciences, U. S. A 76:4350–4354
    [Google Scholar]
  38. Voller A., Bidwell D., Barlett A. 1976; Microplate enzyme immunoassay for the diagnosis of virus infections. In Manual of Clinical Immunology pp 506–512 Edited by Rose N. R., Friedman H. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  39. Wolff L., Scolnick E., Ruscetti S. 1983; Envelope gene of the Friend spleen focus-forming virus: deletion and insertions in 3′ gp70/p15E encoding region have resulted in unique features in the primary structure of its protein product. Proceedings of the National Academy of Sciences, U. S. A 80:4718–4722
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-9-1507
Loading
/content/journal/jgv/10.1099/0022-1317-65-9-1507
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error