1887

Abstract

SUMMARY

Double-stranded cDNA copies of the satellite RNA of tomato black ring virus (strain S) have been cloned in pBR322 and sequenced. From these data the primary nucleotide sequence of the satellite RNA was deduced; it contains 1375 nucleotides and a poly(A) tail. Only one long open reading frame was found which codes for a protein of mol. wt. 47873. This sequence is flanked by a 5′-terminal sequence of 13 nucleotides and a 3′ non-coding region of 87 nucleotides, which contains a sequence that resembles signals for polyadenylation in other RNA molecules.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-65-9-1575
1984-09-01
2024-05-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/65/9/JV0650091575.html?itemId=/content/journal/jgv/10.1099/0022-1317-65-9-1575&mimeType=html&fmt=ahah

References

  1. Ahlquist P., Luckow V., Kaesberg P. 1981; Complete nucleotide sequence of brome mosaic virus RNA 3. Journal of Molecular Biology 153:23–38
    [Google Scholar]
  2. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Bothwell A. L. M., Paskind M., Reth M., Imanishi-Kari T., Rajewsky K., Baltimore D. 1981; Heavy chain variable region contribution to the NPb family of antibodies: somatic mutation evident in a γ 2a variable region. Cell 24:625–637
    [Google Scholar]
  4. Cornelissen B. J. C., Brederode F. T., Moormann R. J. M., Bol J. F. 1983; Complete nucleotide sequence of alfalfa mosaic virus RNA 1. Nucleic Acids Research 11:1253–1265
    [Google Scholar]
  5. Efstratiadis A., Kafatos F. C., Maxam A. M., Maniatis T. 1976; Enzymatic in vitro synthesis of globin genes. Cell 7:279–288
    [Google Scholar]
  6. Fitzgerald M., Shenk T. 1981; The sequence 5′-AAUAAA-3′ forms part of the recognition site for polyadenylation of late SV40 mRNAs. Cell 24:251–260
    [Google Scholar]
  7. Fritsch C., Mayo M. A., Murant A. F. 1978; Translation of the satellite RNA of tomato black ring virus in vitro and in tobacco protoplasts. Journal of General Virology 40:587–593
    [Google Scholar]
  8. Fritsch C., Mayo M. A., Murant A. F. 1980; Translation products of genome and satellite RNAs of tomato black ring virus. Journal of General Virology 46:381–389
    [Google Scholar]
  9. Fritsch C., Koenig I., Murant A. F., Raschke I. H., Mayo M. A. 1984; Comparisons among satellite RNA species from five isolates of tomato black ring virus and one isolate of myrobalan laten ringspot virus. Journal of General Virology 65:289–294
    [Google Scholar]
  10. Goelet P., Lomonossoff G. P., Butler P. J. G., Akam M. E., Gait M. J., Karn J. 1982; Nucleotide sequence of tobacco mosaic virus RNA. Proceedings of the National Academy of Sciences, U. S. A 79:5818–5822
    [Google Scholar]
  11. Harris T. I. R. 1979; The nucleotide sequence of the 5′ end of foot-and-mouth disease virus RNA. Nucleic Acids Research 7:1765–1786
    [Google Scholar]
  12. Harrison B. D. 1958; Relationship between beet ringspot, potato bouquet and tomato black ring viruses. Journal of General Microbiology 18:450–460
    [Google Scholar]
  13. Kitamura N., Semler B. L., Rothberg P. G., Larsen G. R., Adler C. J., Dorner A. J., Emini E. A., Hanecak R., Lee J. J., Van Der Werf S., Anderson C. W., Wimmer E. 1981; Primary structure, gene organization and polypeptide expression of poliovirus RNA. Nature, London 291:547–553
    [Google Scholar]
  14. Koeing I., Fritsch C. 1982; A protein linked at the 5′ end of satellite and genomic tomato black ring virus RNAs: study of in vitro translation after protease treatment. Journal of General Virology 60:343–353
    [Google Scholar]
  15. Kozak M. 1981; Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Research 9:5233–5252
    [Google Scholar]
  16. Lomonossoff G. P., Shanks M. 1983; The nucleotide sequence of cowpea mosaic virus B RNA. EMBO Journal 2:2253–2258
    [Google Scholar]
  17. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  18. Mayo M. A., Barker H., Harrison B. D. 1979; Polyadenylate in the RNA of five nepoviruses. Journal of General Virology 43:603–610
    [Google Scholar]
  19. Mayo M. A., Barker H., Harrison B. D. 1982; Specificity and properties of the genome-linked proteins of nepoviruses. Journal of General Virology 59:149–162
    [Google Scholar]
  20. Murant A. F., Taylor M. 1978; Estimates of molecular weights of nepovirus RNA species by polyacrylamide gel electrophoresis under denaturing conditions. Journal of General Virology 41:53–61
    [Google Scholar]
  21. Murant A. F., Mayo M. A., Harrison B. D., Goold R. A. 1973; Evidence for two functional RNA species and a satellite RNA in tomato black ring virus. Journal of General Virology 19:275–278
    [Google Scholar]
  22. Murant A. F., Taylor M., Duncan G. H., Raschke J. H. 1981; Improved estimates of molecular weight of plant virus RNA by agarose gel electrophoresis and electron microscopy after denaturation with glyoxal. Journal of General Virology 53:321–332
    [Google Scholar]
  23. Racaniello V. R., Baltimore D. 1981; Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome. Proceedings of the National Academy of Sciences, U. S. A 78:4887–4891
    [Google Scholar]
  24. Robinson D. J. 1982; Apparent lack of nucleotide sequence homology between the satellite and genome RNA species of tomato black ring virus. Journal of General Virology 58:453–456
    [Google Scholar]
  25. Smith D. R., Calvo J. M. 1980; Nucleotide sequence of the E. coli gene coding for dihydrofolate reductase. Nucleic Acids Research 8:2255–2274
    [Google Scholar]
  26. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U. S. A 77:5201–5205
    [Google Scholar]
  27. Van Vloten-Doting L., Dubelaar M., Bol J. F. 1982; Open reading frame in the minus strand of two plus type RNA viruses. Plant Molecular Biology 1:155–158
    [Google Scholar]
  28. Van Wezenbeek P., Verver J., Harmsen J., Vos P., Van Kammen A. 1983; Primary structure and gene organization of the middle component RNA of cowpea mosaic virus. EMBO Journal 2:941–946
    [Google Scholar]
  29. Vogelstein B., Gillespie D. 1979; Preparative and analytical purification of DNA from agarose. Proceedings of the National Academy of Sciences, U. S. A 76:615–619
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-65-9-1575
Loading
/content/journal/jgv/10.1099/0022-1317-65-9-1575
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error