1887

Abstract

SUMMARY

Formation of proviral DNAs by B-tropic murine leukaemia viruses (MLVs) was examined in N-type and dually permissive mutant cells derived from two inbred mouse strains, DDD and G, both of which are N-type. In the N-type cells, formation of circular proviral DNA was strongly suppressed relative to that of linear DNA. Mutation resulting in loss of the N-type Fv-1 restriction resulted in efficient formation of circular DNA by the previously restricted B-tropic MLV. This showed that Fv-1 restriction and inhibition of closed circular DNA formation were controlled by the same gene. The efficiency of formation of circular proviral DNA by the defective Kirsten murine sarcoma virus was determined by the tropism of the helper virus.

Keyword(s): Fv-1 , MLV and proviral DNA
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/content/journal/jgv/10.1099/0022-1317-66-10-2265
1985-10-01
2024-04-26
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References

  1. Axelrad A. 1968; Genetic and cellular basis of susceptibility or resistance to Friend leukemia virus infection in mice. Canadian Cancer Conference 8:313–343
    [Google Scholar]
  2. Berns A. J. M., Lai M. H. T., Bosselman R. A., McKennett M. A., Bacheler L. T., Fan H. R., Maandag E. C., Putten H. V. D., Verma I. M. 1980; Molecular cloning of unintegrated and a portion of integrated Moloney murine leukemia viral DNA in bacteriophage lambda. Journal of Virology 36:254–263
    [Google Scholar]
  3. Chinsky J., Soeiro R. 1981; Fv-1 host restriction of Friend leukemia virus: analysis of unintegrated proviral DNA. Journal of Virology 40:45–55
    [Google Scholar]
  4. Desgroseillers L., Jolicoeur P. 1983; Physical mapping of the Fv-1 tropism host range determinant of BALB/c murine leukemia viruses. Journal of Virology 48:685–696
    [Google Scholar]
  5. Faller D., Hopkins N. 1977; RNase Tl-resistant oligonucleotides of B-tropic murine leukemia virus from BALB/c and five of its NB-tropic derivatives. Journal of Virology 23:188–195
    [Google Scholar]
  6. Gautsch J., Elder J., Schindler J., Jemsen F., Lerner R. 1978; Structural markers on core protein p30 of murine leukemia virus: functional correlation with Fv-1 tropism. Proceedings of the National Academy of Sciences, U.S.A 75:4170–4174
    [Google Scholar]
  7. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  8. Hopkins N., Schindler J., Hynes R. 1977; Six NB-tropic murine leukemia viruses derived from a B-tropic virus of BALB/c have altered p30. Journal of Virology 21:309–318
    [Google Scholar]
  9. Jolicoeur P., Rassart E. 1980; Effect of Fv-1 gene product on synthesis of linear and supercoiled viral DNA in cells infected with murine leukemia virus. Journal of Virology 33:183–195
    [Google Scholar]
  10. Ou C., Boone L., Koh C., Tennant R., Yang W. 1983; Nucleotide sequences of gag-pol regions that determine the Fv-1 host range property of BALB/c N-tropic and B-tropic murine leukemia viruses. Journal of Virology 48:779–784
    [Google Scholar]
  11. Rassart E., DesGroseillers L., Jolicoeur P. 1981; Molecular cloning of B- and N-tropic endogenous BALB/c murine leukemia virus circular DNA intermediates: isolation and characterization of infectious recombinant clones. Journal of Virology 39:162–171
    [Google Scholar]
  12. Rein A., Kashmiri S., Bassin R., Gerwin B., Duran-Troise G. 1976; Phenotypic mixing between N- and B-tropic murine leukemia viruses: infectious particles with dual sensitivity to Fv-1 restriction. Cell 7:373–379
    [Google Scholar]
  13. Rommelaere J., Donis-Keller H., Hopkins N. 1979; RNA sequencing provides evidence for allelism of determinants of N-, B-, or NB-tropism of murine leukemia viruses. Cell 16:43–50
    [Google Scholar]
  14. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  15. Tsuchida N., Ryder T., Ohtsubo E. 1982; Nucleotide sequence of the oncogene encoding the p21 transforming protein of Kirsten murine sarcoma virus. Science 217:937–939
    [Google Scholar]
  16. Varmus H. E. 1983; Retroviruses. In Mobile Genetic Elements411–503 Edited by Shapiro J. A. New York: Academic Press;
    [Google Scholar]
  17. Yang W., Kiggans J., Yang D., Ou C., Tennant R., Brown A., Bassin R. 1980; Synthesis and circularization of N- and B-tropic retroviral DNA in Fv-1 permissive and restrictive mouse cells. Proceedings of the National Academy of Sciences, U.S.A 77:2994–2998
    [Google Scholar]
  18. Yoshikura H. 1968; Requirement of cellular DNA synthesis for the growth of Friend leukemia virus. Experimental Cell Research 52:445–450
    [Google Scholar]
  19. Yoshikura H., Yoshida M. 1978; Intracellular restriction of ecotropic murine leukemia virus in rat NRK cells and its abolishment by adaptation. Journal of Virology 27:612–618
    [Google Scholar]
  20. Yoshikura H., Naito Y., Moriwaki K. 1978; Unstable resistance of G mouse fibroblasts to ecotropic murine leukemia virus infection. Journal of Virology 29:1078–1086
    [Google Scholar]
  21. Yoshikura H., Tejima S., Kuchino K., Segqwa K., Odaka T. 1982; Characterization of N-type and dually permissive cells segregated from mouse fibroblasts whose Fv-1 phenotype could be modified by another independently segregating gene(s). Journal of Virology 41:145–152
    [Google Scholar]
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